Physicochemical characteristics of emulsions during fat digestion in human stomach and duodenum

Armand, Martine; Borel, Patrick; Pasquier, B; Dubois, C.; Senft, M.; André, M.; Peyrot, J; Salducci, J; Lairon, Denis

doi:10.1152/ajpgi.1996.271.1.g172

Cited by 178 publications

(224 citation statements)

References 14 publications

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“…This is particularly true for dietary lipids, which are basically insoluble in the aqueous medium present in most foodstuffs and in the digestive tract. Thus, lipid moieties are organized in different kind of complex structures (emulsified droplets with different compositions and sizes, multilamelar structures, vesicles) that will determine the rate of fat digestion by gut lipases (Armand et al, 1996(Armand et al, , 1999. This can affect the rate of gastric emptying as well as kinetics of postprandial occurrence of chylomicrons in the circulation (Sakr et al, 1997;Armand et al, 1999).…”

Section: Physicochemical Properties Of Test Mealsmentioning

confidence: 99%

Methodology for studying postprandial lipid metabolism

2007

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Background: Postprandial lipid metabolism in humans has deserved much attention during the last two decades. Although fasting lipid and lipoprotein parameters reflect body homeostasis to some extent, the transient lipid and lipoprotein accumulation that occurs in the circulation after a fat-containing meal highlights the individual capacity to handle an acute fat input. An exacerbated postprandial accumulation of triglyceride-rich lipoproteins in the circulation has been associated with an increased cardiovascular risk. Methods: The important number of studies published in this field raises the question of the methodology used for such postprandial studies, as reviewed. Results: Based on our experiences, the present review reports and discuss the numerous methodological issues involved to serve as a basis for further works. These aspects include aims of the postprandial tests, size and nutrient composition of the test meals and background diets, pre-test conditions, characteristics of subjects involved, timing of sampling, suitable markers of postprandial lipid metabolism and calculations. Conclusion: In conclusion, we stress the need for standardization of postprandial tests.

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Section: Physicochemical Properties Of Test Mealsmentioning

confidence: 99%

Methodology for studying postprandial lipid metabolism

2007

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“…Total bile acid concentration in HIF fed 90 was 9.2 mM (Table 4). Using enzymatic methods, Armand et al (19) reported mean values of 6.7-13.4 mM up to 4 h after the administration of a meal, whereas Kalantzi et al (6) reported values in which the medians dropped from 11.2 mM at 30 min to 5.2 mM at 180 min post meal administration. Using an HPLC-ELSD method (17) the mean 6 SD total bile acid level in the fed jejunum was estimated to be 8 6 0.1 mM.…”

Section: Methods Validationmentioning

confidence: 99%

Determination of intralumenal individual bile acids by HPLC with charged aerosol detection

Vertzoni

Archontaki

Reppas

2008

Journal of Lipid Research

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An isocratic HPLC charged aerosol detector (CAD) method was developed, validated, and applied for the determination of individual bile acids in human gastric and duodenal aspirates. The method requires a low volume of aspirates (50-100 ml) and minimal sample pretreatment. A Hypersil BDS RP-C 18 column (250 3 4.6 mm, 5 mm particle size) was equilibrated with a mobile phase composed of methanol-[ammoniun formate 20 mM, formic acid 0.5%, triethylamine 0.2% (pH 3)] 67:33 v/v. Its flow rate was 1 ml/min. The elution times for taurocholate, glycocholate, taurochenodeoxycholate, ursodeoxycholate, glycochenodeoxycholate, cholate, and glycodeoxycholate were approximately 9.9, 16.2, 18.2, 21.3, 31.6, 34.5, and 38.5 min, respectively. Calibration curves in the mobile phase were constructed in the concentration range of 0.5-500 mM. Limits of detection and quantification were in the range of 0.07-0.60 mM and 0.20-1.80 mM, respectively. This method was applied first, in gastric aspirates collected in the fasted state, in which bile acid presence is minimal and, second, in duodenal aspirates collected in the fed state, in which a large number of potentially interfering compounds exists. Intra-day relative standard deviation in fasted gastric aspirates and in fed duodenal aspirates was less than 2.2% and 6.0%, respectively.-Vertzoni, M., H. Archontaki, and C. Reppas. Determination of intralumenal individual bile acids by HPLC with charged aerosol detection. J. Lipid Res. 2008Res. . 49: 2690Res. -2695

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“…Each procedure started at 07:30 AM after the subjects had fasted overnight for 12 h. As previously described (1,5), each subject was intubated with a singlelumen nasogastric tube (16 Fr, 122 cm, Sherwood Medical Argyle, Tullamore, Ireland) and a single-lumen nasoduodenal tube (duodenographie bilboa-dotter set, 12 Fr, 145 cm, William Cook Europe, Bjaeverkov, Denmark). The stomach tube was located 45-50 cm from the nose in the stomach at the corpus-antrum junction.…”

Section: Subjectsmentioning

confidence: 99%

Processing of vegetable-borne carotenoids in the human stomach and duodenum

Tyssandier

Reboul

Dumas

et al. 2003

American Journal of Physiology-Gastrointestinal and Liver Physi

237

186

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Carotenoids are thought to diminish the incidence of certain degenerative diseases, but the mechanisms involved in their intestinal absorption are poorly understood. Our aim was to obtain basic data on the fate of carotenoids in the human stomach and duodenum. Ten healthy men were intragastrically fed three liquid test meals differing only in the vegetable added 3 wk apart and in a random order. They contained 40 g sunflower oil and mashed vegetables as the sole source of carotenoids. Tomato purée provided 10 mg lycopene as the main carotenoid, chopped spinach (10 mg lutein), and carrot purée (10 mg β-carotene). Samples of stomach and duodenal contents and blood samples were collected at regular time intervals after meal intake. all -trans and ciscarotenoids were assayed in stomach and duodenal contents, in the fat and aqueous phases of those contents, and in chylomicrons. The cis-trans β-carotene and lycopene ratios did not significantly vary in the stomach during digestion. Carotenoids were recovered in the fat phase present in the stomach during digestion. The proportion of all -trans carotenoids found in the micellar phase of the duodenum was as follows (means ± SE): lutein (5.6 ± 0.4%), β-carotene (4.7 ± 0.3%), lycopene (2.0 ± 0.2%). The proportion of 13- cis β-carotene in the micellar phase was significantly higher (14.8 ± 1.6%) than that of the all -trans isomer (4.7 ± 0.3%). There was no significant variation in chylomicron lycopene after the tomato meal, whereas there was significant increase in chylomicron β-carotene and lutein after the carrot and the spinach meals, respectively. There is no significant cis-transisomerization of β-carotene and lycopene in the human stomach. The stomach initiates the transfer of carotenoids from the vegetable matrix to the fat phase of the meal. Lycopene is less efficiently transferred to micelles than β-carotene and lutein. The very small transfer of carotenoids from their vegetable matrices to micelles explains the poor bioavailability of these phytomicroconstituents.

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Physicochemical characteristics of emulsions during fat digestion in human stomach and duodenum

Cited by 178 publications

References 14 publications

Methodology for studying postprandial lipid metabolism

Methodology for studying postprandial lipid metabolism

Determination of intralumenal individual bile acids by HPLC with charged aerosol detection

Processing of vegetable-borne carotenoids in the human stomach and duodenum

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