Physicochemical characteristics of chia seed (Salvia hispanica) protein hydrolysates produced using ultrasonication followed by microwave-assisted hydrolysis

Urbizo-Reyes, Uriel; Martín-González, M.F. San; Garcia-Bravo, José; Vigil, Aurelio López Malo; Liceaga, Andrea M.

doi:10.1016/j.foodhyd.2019.105187

Cited by 99 publications

(47 citation statements)

References 53 publications

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“…Although these values were not as low as the IC 50 reported for Captopril (0.02 μg/mL), our results were 10-fold lower from those previously reported for canary seed peptides, ranging between 217 and 332 μg/mL (Estrada-Salas et al 2014;Valverde et al 2017). The values were also lower than those reported for ultra-filtered (< 3 kDa) canary seed peptides (IC 50 = 0.33 mg/mL), chia seed peptides (IC 50 = 0.42 mg/mL), wheat protein hydrolysates (IC 50 = 0.42-2.22 mg/mL), and similar to those reported for oats (IC 50 = 0.030-0.050 mg/mL) (Cheung et al 2009;Jia et al 2010;Mason 2019;Urbizo-Reyes et al 2019). In our study, the use of Alcalase, an endo-protease, influenced the type of peptides released.…”

Section: Ace Inhibitory Activitysupporting

confidence: 65%

“…Proteolysis was carried out following the procedure described by Urbizo-Reyes et al (2019). Briefly, canary seed powder was diluted to obtain (22.5 mg of protein/ mL) and homogenized using a Sorvall Omni Mixer (Norwalk, CT, U.S.A).…”

Section: Production Of Canary Seed Peptides (Csp)mentioning

confidence: 99%

“…The ACE-inhibitory activity was measured, according to Urbizo-Reyes et al (2019). CSP fractions were dissolved in phosphate buffer (100 mM, pH 8.3) with 300 mM sodium chloride.…”

Section: Angiotensin Converting Enzyme (Ace) Inhibitory Activitymentioning

confidence: 99%

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Hairless canary seeds (Phalaris canariensis L.) as a potential source of antioxidant, antihypertensive, antidiabetic, and antiobesity biopeptides

Urbizo-Reyes

Aguilar-Toalá

Liceaga

2021

Food Prod Process and Nutr

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Rising consumer concerns with synthetic drugs to treat non-communicable diseases (NCDs) have promoted a shift towards using natural biological active constituents that offer similar health benefits. Hairless canary seed (Phalaris canariensis L) is an emerging crop traditionally used in Mexico to treat NCDs. Peptides liberated during simulated digestion of canary seed protein are believed to be responsible for their biological activity; however, no studies have shown the effect of controlled protein hydrolysis using commercial proteases on canary seed protein’s biological activity. Therefore, this study aimed to explore the in vitro antihypertensive, antidiabetic, and anti-obesity activity of canary seed peptides derived from proteolysis with Alcalase®. Protein fractions were primarily composed of prolamins (54.07 ± 1.8%), glutelins (32.19 ± 3.18%), globulins (5.97 ± 0.52%) and albumins (5.97 ± 0.52%). The < 3 kDa and 3–10 kDa peptide fractions showed the highest inhibition capacity (p < 0.05) towards angiotensin-converting enzyme (IC50= 0.028–0.032 mg/mL) lipase (IC50= 2.15–2.27 mg/mL), α-glucosidase (IC50= 0.82–1.15 mg/mL), and dipeptidyl-peptidase-IV (IC50= 1.27–1.60 mg/mL). Additionally, these peptide fractions showed high antioxidant activity against DPPH (134.22–150.66 μmol TE/mg) and ABTS (520.92–813.33 μmol TE/mg). These results provide an insight into the potential development of functional foods using commercial enzymatic hydrolysis of canary seed proteins for treating hypertension, type-2 diabetes, and obesity.

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Section: Ace Inhibitory Activitysupporting

confidence: 65%

Section: Production Of Canary Seed Peptides (Csp)mentioning

confidence: 99%

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Hairless canary seeds (Phalaris canariensis L.) as a potential source of antioxidant, antihypertensive, antidiabetic, and antiobesity biopeptides

Urbizo-Reyes

Aguilar-Toalá

Liceaga

2021

Food Prod Process and Nutr

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“…The results have shown that the optimal method used for investigating the protein content in chia seeds depends on the desired product. Following this, Urbizo-Reyes et al [33] investigated how the biological and functional properties of chia seeds' protein hydrolysate improves when using ultrasonication to remove mucilage and how microwave-assisted enzymatic hydrolysis are generating bioactive and functional chia seeds' peptide.…”

Section: Protein Contentmentioning

confidence: 99%

Chia Seeds (Salvia Hispanica L.): An Overview—Phytochemical Profile, Isolation Methods, and Application

et al. 2019

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Chia (Salvia hispanica L.) is a small seed that comes from an annual herbaceous plant, Salvia hispanica L. In recent years, usage of Chia seeds has tremendously grown due to their high nutritional and medicinal values. Chia was cultivated by Mesopotamian cultures, but then disappeared for centuries until the middle of the 20th century, when it was rediscovered. Chia seeds contain healthy ω-3 fatty acids, polyunsaturated fatty acids, dietary fiber, proteins, vitamins, and some minerals. Besides this, the seeds are an excellent source of polyphenols and antioxidants, such as caffeic acid, rosmarinic acid, myricetin, quercetin, and others. Today, chia has been analyzed in different areas of research. Researches around the world have been investigating the benefits of chia seeds in the medicinal, pharmaceutical, and food industry. Chia oil is today one of the most valuable oils on the market. Different extraction methods have been used to produce the oil. In the present study, an extensive overview of the chemical composition, nutritional properties, and antioxidant and antimicrobial activities, along with extraction methods used to produce chia oil, will be discussed.

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“…leaf protein hydrolysate that exhibited cellular antioxidant activity (1706, 1501 and 2204 lM quercetin equivalents/100 g peptide, respectively). Likewise, Urbizo-Reyes et al (2019) reported the cellular antioxidant activity of chia seed hydrolysates. The authors found that the highest cellular antioxidant activity (% per mg of protein) was achieved by the sequential (94.76%) and microwave-assisted (93.13%) proteolysis.…”

Section: Mammalian Cell Line Models Used To Evaluate Cellular Antioxidant Activitymentioning

confidence: 99%

Cellular antioxidant effect of bioactive peptides and molecular mechanisms underlying: beyond chemical properties

Aguilar-Toalá

Liceaga

2020

Int J of Food Sci Tech

Self Cite

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Bioactive peptides are known for their chemical antioxidant activities. However, the importance of cellular antioxidant peptides has relatively been overlooked; nevertheless, scientific evidence supporting their potential to prevent chronic diseases associated with oxidative stress is progressively increasing. Cellular in vitro and in vivo studies have demonstrated that antioxidant bioactive peptides possess cellular antioxidant activity, decrease oxidative stress biomarkers (e.g. lipid peroxidation, intracellular ROS levels, apoptosis), increase diverse antioxidant enzymes' activities and modulate levels of antioxidant molecules. These cellular in vitro bioactive properties and in vivo health effects suggest that antioxidant peptides could be used as components of functional foods and contribute to health promotion by improving specific physiological functions. This review highlights the scientific evidence about cellular antioxidant activity of bioactive peptides, their protective effect and underlying molecular mechanisms of action. It also describes the underlying antioxidant mechanisms and the structure-function relationship of antioxidant peptides.

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Physicochemical characteristics of chia seed (Salvia hispanica) protein hydrolysates produced using ultrasonication followed by microwave-assisted hydrolysis

Cited by 99 publications

References 53 publications

Hairless canary seeds (Phalaris canariensis L.) as a potential source of antioxidant, antihypertensive, antidiabetic, and antiobesity biopeptides

Hairless canary seeds (Phalaris canariensis L.) as a potential source of antioxidant, antihypertensive, antidiabetic, and antiobesity biopeptides

Chia Seeds (Salvia Hispanica L.): An Overview—Phytochemical Profile, Isolation Methods, and Application

Cellular antioxidant effect of bioactive peptides and molecular mechanisms underlying: beyond chemical properties

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