Physicochemical Changes in Endotoxin Associated With Loss of Biological Potency

Ribi, Edgar; Haskins, W. T.; Milner, Kelsey C.; Anacker, R. L.; Ritter, Daniel B.; Goode, Granville; Trapani, Robert-John; Landy, Maurice

doi:10.1128/jb.84.4.803-814.1962

Cited by 53 publications

(27 citation statements)

References 11 publications

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“…The finding of decreased in vitro biological activity in lipid A preparations has also been reported by Nowotny et al (17) and Wahl et al (30) and is most likely due to a general loss of potency observed in LPS preparations having undergone acid hydrolysis (8,20). In addition, we have found that heat treatment markedly decreases the cytotoxic properties of LPS, and since production of lipid A involves boiling for 45 min, this might also explain its loss of potency.…”

Section: Discussionsupporting

confidence: 89%

Analysis of the effects of lipopolysaccharide on macrophages: differential phagocytic responses of C3H/HeN and C3H/HeJ macrophages in vitro

Marshall

Rosenstreich

1979

Infect Immun

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The phenomenon of lipopolysaccharide (LPS)-induced in vitro macrophage cytotoxicity has been reported by a number of investigators but has often been difficult to reproduce and to quantitate. In this report, we have examined the effect of LPS on the ability of macrophages to ingest 51Cr-labeled, opsonized sheep erythrocytes as a method for examining the direct toxic effects of LPS on macrophages in vitro. By using this assay, we can clearly discriminate between LPS responder C3H/HeN macrophages and LPS nonresponder C3H/HeJ macrophages and demonstrate that LPS induces a profound inhibition of Fc-mediated phagocytosis in LPS responsive macrophages. Furthermore, low concentrations of LPS stimulate phagocytosis in macrophages derived for C3H/HeJ mice. The lipid A moiety of the LPS is responsible for the observed enhancement or inhibition of Fc-mediated phagocytosis. This assay was more sensitive than LPSinduced cytotoxicity, since inhibition of phagocytosis was detectable in cultures of LPS-sensitive macrophages even when cytotoxicity, assessed by trypan blue exclusion, was not. Thus, this assay represents an extremely sensitive method for analyzing the direct effects of LPS on macrophages.INFECT. IMMUN. on August 5, 2020 by guest http://iai.asm.org/ Downloaded from

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Section: Discussionsupporting

confidence: 89%

Analysis of the effects of lipopolysaccharide on macrophages: differential phagocytic responses of C3H/HeN and C3H/HeJ macrophages in vitro

Marshall

Rosenstreich

1979

Infect Immun

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“…Both degraded antigens made a line close to the antiserum well. These results are similar to the findings of Ribi et al (8).…”

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confidence: 93%

High Thiobarbiturate-Positive O Antigen of Escherichia coli

1971

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“…strongly suggest a relationship between the two precipitinogens in LPS-sup. from B. melaninogenicus strain B10 similar to that observed in endotoxin preparations from Enterobacteriaceae by Ribi et al (13) and in Fusobacterium by Kristoffersen (9). This would imply that the rapidly diffusing prccipitinogen corresponds to the so-called "acid hapten".…”

Section: Gel Filtration On Bio-gelsupporting

confidence: 65%

Lipopolysaccharide From Bacteroides Melaninogenicus Isolated From the Supernatant Fluid After Ultracentrifugation of the Water Phase Following Phenol‐water Extraction

Hofstad

Kristoffersen

1971

Acta Pathologica Microbiologica Scandinavica Section B Microbio

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Lipopolysaccharide (LPS) has been isolated from the supernatant fluid aftcr ultracentrifugation of the water phase following phenol-water extraction of Bncteroides meloninogenicus, strain B10. The purification procedure included digestion of the supernatant fluid with ribonuclease and deoxyribonuclease, gel filtration on Bio-Gel A-1.5 m and ion exchange chromatography on DEAE-cellulose. The prepared LPS did not contain heptose or 2-keto-3-deoxyoctulosonic acid. The sugar components identified were galactose, glucose, mannose, rhamnose, traces of fiicose, galactosamine and glucosamine. Neutral sugar and lipid constituted the main parts or' the LPS. The isolated LPS sensitized sheep erythrocytes to agglutination in homologous antiserum. T w o agar precipitation lines were observed, one of which may correspond to an "acid haptcn".

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Physicochemical Changes in Endotoxin Associated With Loss of Biological Potency

Abstract: changes in endotoxin associated with loss of biological potency. J. Bacteriol. 84:803-814. 1962.-The preparation of endotoxins whose gross chemical composition approached that of refined polysaccharide haptenes raised anew the question of which

Cited by 53 publications

References 11 publications

Analysis of the effects of lipopolysaccharide on macrophages: differential phagocytic responses of C3H/HeN and C3H/HeJ macrophages in vitro

Analysis of the effects of lipopolysaccharide on macrophages: differential phagocytic responses of C3H/HeN and C3H/HeJ macrophages in vitro

High Thiobarbiturate-Positive O Antigen of Escherichia coli

Lipopolysaccharide From Bacteroides Melaninogenicus Isolated From the Supernatant Fluid After Ultracentrifugation of the Water Phase Following Phenol‐water Extraction

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