Physicochemical, biological and drug-release properties of gallium crosslinked alginate/nanoparticulate bioactive glass composite films

Abstract

The aim of this work was to develop biodegradable and bioactive materials with sufficient structural integrity and prophylaxis effect against infection based on alginate-bioactive glass composite. The incorporation of bioactive glass nanoparticles (NBG) into Ga-crosslinked alginate films significantly improved their mechanical properties when compared with films fabricated with micron-sized bioactive glass particles. In addition, Ga-alginate films containing NBG induced a bacteriostatic effect in vitro towards S. aureus due to the presence of Ga ions (Ga 3+ ), whose release is controlled solely by crosslinking the ion with alginate. Biomineralization studies in simulated body fluid suggested the deposition of hydroxyapatite on the surface of the films indicating their bioactive nature. In addition, the films were shown to feature biocompatibility toward osteoblast-like cells. Thus, it was shown that Ga-crosslinked composite films possessed relevant physicochemical, biological and controlled bacteriostatic effects which make these materials promising candidates for bone tissue engineering applications.

Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds

“…Based on this fact, our group envisaged the possibility to control the release of the chosen multivalent ions in a sustained manner from a cross-linked site. In that sense, gallium cross-linked alginate/ nanoparticulate bioactive glass composite two-dimensional (2D) scaffolds were successfully developed [43]. In addition, the incorporation of metallic ions into scaffolds for tissue engineering is being increasingly considered [35,[43][44][45][46][47][48][49].…”

“…The possibility to combine both bioactive glass nanoparticles (Nbg) and alginate (Alg) allows synergizing the advantages of these biomaterials to develop composite scaffolds for BTE with suitable properties such as biodegradability, biocompatibility and mechanical strength [43,61,62]. This work builds upon the mentioned previous work of gallium cross-linked alginate/nanoparticulate bioactive glass composite 2D scaffolds, focusing on novel combinations of Nbg and Alg to achieve tuneable release capabilities of divalent ions-such as Cu 2þ and Ca 2þ -incorporated by cross-linking them with Alg in the composite biomaterials.…”

Bioactive Nanocomposites Containing Silicate Phases for Bone Replacement and Regeneration

“…It was suggested that the biocompatible composite scaffolds can serve as an appropriate bioactive matrix for periodontal tissue regeneration [81]. In a recent study, the physicochemical, biological, and drug-release properties of gallium-cross-linked alginate/nanoparticulate BG composite films were investigated [82]. The aim of the work was to develop biodegradable and bioactive materials with both sufficient structural integrity and prophylaxis effect against infection.…”

Development and Validation of a Capillary Zone Electrophoresis Method for the Determination of Calcium in Composite Biomaterials

“…Calcium cross-linked alginate films containing bioactive glass nanoparticles intended for BTE applications were similarly prepared as mentioned in a previous work [5]. Different phosphate salts containing contra-ions such as Na …”

“…These considerations prompted the incorporation of MITA into different releasing systems, e.g. scaffolds, intended for therapeutic applications [1][2][5][6]. Particularly, a large amount of biomaterials employed in the elaboration of scaffolds for bone tissue engineering (BTE) contain calcium ions, Ca 2+ [7][8][9][10][11][12][13][14][15].…”