Physicochemical Aspects of Deposition of Streptococcus thermophilus B to Hydrophobic and Hydrophilic Substrata in a Parallel Plate Flow Chamber

Meinders, Jm; Mei, van der Henny C.; Busscher, Henk J.

doi:10.1006/jcis.1994.1177

Cited by 44 publications

(47 citation statements)

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“…However, the residence time for individual cells was not measured. The rapid transition from reversible to irreversible adhesion shown here and in previous studies (19,26,36) suggests that, contrary to the accepted model (18,23), physiological responses by the bacteria were not responsible for the development of irreversible cell adhesion. However, this does not discount the possibility that physiological responses such as exopolysaccharide production could be induced by irreversible adhesion (3).…”

Section: Discussioncontrasting

confidence: 83%

“…While some studies have indicated a role of physiological responses by the organism in the process (20), other studies indicate that rapid aging of cell-substratum bonds is responsible for the development of irreversible adhesion (24,26). The analysis of the residence time distribution done in this study suggests a rapid transition from reversible to irreversible adhesion.…”

Section: Discussionmentioning

confidence: 59%

“…Although the forces involved in bacterial adhesion are not fully understood, it is evident from numerous studies of different systems that substratum wettability is an important factor in bacterial adhesion (9,12,13,26), as well as in protein adsorption (33) and the attachment of eukaryotic cells (21,39). Many studies of bacterial adhesion and substratum wettability have utilized different materials, e.g., glass, metals, and polymers, to represent substrata with different wettabilities (12,38,40).…”

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confidence: 99%

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Bacterial adhesion to hydroxyl- and methyl-terminated alkanethiol self-assembled monolayers

Wiencek

Fletcher

1995

J Bacteriol

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The attachment of bacteria to solid surfaces is influenced by substratum chemistry, but to determine the mechanistic basis of this relationship, homogeneous, well-defined substrata are required. Self-assembled monolayers (SAMs) were constructed from alkanethiols to produce a range of substrata with different exposed functional groups, i.e., methyl and hydroxyl groups and a series of mixtures of the two. Percentages of hydroxyl groups in the SAMs and substratum wettability were measured by X-ray photoelectron spectroscopy and contact angles of water and hexadecane, respectively. SAMs exhibited various substratum compositions and wettabilities, ranging from hydrophilic, hydroxyl-terminated monolayers to hydrophobic, methyl-terminated monolayers. The kinetics of attachment of an estuarine bacterium to these surfaces in a laminar flow chamber were measured over periods of 120 min. The initial rate of net adhesion, the number of cells attached after 120 min, the percentage of attached cells that adsorbed or desorbed between successive measurements, and the residence times of attached cells were quantified by phase-contrast microscopy and digital image processing. The greatest numbers of attached cells occurred on hydrophobic surfaces, because (i) the initial rates of adhesion and the mean numbers of cells that attached after 120 min increased with the methyl content of the SAM and the contact angle of water and (ii) the percentage of cells that desorbed between successive measurements (ca. 2 min) decreased with increasing substratum hydrophobicity. With all surfaces, 60 to 80% of the cells that desorbed during the 120-min exposure period had residence times of less than 10 min, suggesting that establishment of firm adhesion occurred quickly on all of the test surfaces.The consequences of bacterial adhesion to nonbiological surfaces can be beneficial or deleterious depending on the situation. Bioreactor and biofilter systems rely on the adhesion and growth of bacterial cells on support materials for effective operation (4). Also, bacterial biofilms can be utilized to promote the attachment of other organisms in the aquaculture of invertebrates (10). In marine and estuarine environments, irreversible attachment and the subsequent growth of bacteria on surfaces can affect the attachment of other organisms, often leading to the fouling of heat exchangers, ship hulls, and other synthetic surfaces (2, 18, 23). As a result, the study of bacterial adhesion and the effects of substratum surface properties has received considerable attention.Although the forces involved in bacterial adhesion are not fully understood, it is evident from numerous studies of different systems that substratum wettability is an important factor in bacterial adhesion (9, 12, 13, 26), as well as in protein adsorption (33) and the attachment of eukaryotic cells (21, 39). Many studies of bacterial adhesion and substratum wettability have utilized different materials, e.g., glass, metals, and polymers, to represent substrata with different wettabilities...

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Section: Discussioncontrasting

confidence: 83%

Section: Discussionmentioning

confidence: 59%

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confidence: 99%

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Bacterial adhesion to hydroxyl- and methyl-terminated alkanethiol self-assembled monolayers

Wiencek

Fletcher

1995

J Bacteriol

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“…Previous studies have shown that shear stress increases the duration of adhesion of both P. aeruginosa and Escherichia coli biofilms to coated surfaces. 30,31 This reduction in the amount of bacteria seen on the ETTs in the in vitro model could be an effect of changes in the physical properties of the biofilm in reaction to shear stress. 32 High shear forces have been shown in the literature to favor the creation of tight, rounded microcolonies of bacteria.…”

Section: Discussionmentioning

confidence: 98%

Decreased <em>Pseudomonas aeruginosa</em> biofilm formation on nanomodified endotracheal tubes: a dynamic lung model

Machado¹,

Webster²

2016

IJN

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Ventilator-associated pneumonia (VAP) is a serious complication of mechanical ventilation that has been shown to be associated with increased mortality rates and medical costs in the pediatric intensive care unit. Currently, there is no cost-effective solution to the problems posed by VAP. Endotracheal tubes (ETTs) that are resistant to bacterial colonization and that inhibit biofilm formation could provide a novel solution to the problems posed by VAP. The objective of this in vitro study was to evaluate differences in the growth of Pseudomonas aeruginosa on unmodified polyvinyl chloride (PVC) ETTs and on ETTs etched with a fungal lipase, Rhizopus arrhizus , to create nanoscale surface features. These differences were evaluated using an in vitro model of the pediatric airway to simulate a ventilated patient in the pediatric intensive care unit. Each experiment was run for 24 hours and was supported by computational models of the ETT. Dynamic conditions within the ETT had an impact on the location of bacterial growth within the tube. These conditions also quantitatively affected bacterial growth especially within the areas of tube curvature. Most importantly, experiments in the in vitro model revealed a 2.7 log reduction in the number (colony forming units/mL) of P. aeruginosa on the nanoroughened ETTs compared to the untreated PVC ETTs after 24 hours. This reduction in total colony forming units/mL along the x -axis of the tube was similar to previous studies completed for Staphylococcus aureus . Thus, this dynamic study showed that lipase etching can create surface features of nanoscale roughness on PVC ETTs that decrease bacterial attachment of P. aeruginosa without the use of antibiotics and may provide clinicians with an effective and inexpensive tool to combat VAP.

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“…Although bacterial desorption is measurable (Meinders et al, 1994), desorption rates are usually small and in the order of s-l, which is negligible compared to the adsorption rate. The difference in initial deposition rates between bacteria suspended in PBS and in PBS supplemented with nutrient broth may be associated with a growth component (Bryers 8c Characklis, 1992).…”

Section: Discussionmentioning

confidence: 99%

Adhesion of Pseudomonas aeruginosa to silicone rubber in a parallel plate flow chambe in the absence and presence of nutrient broth

et al. 1997

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The physico-chemical cell-surface properties of Pseudomonas aeruginosa AKI and i t s adhesion to silicone rubber under flow were compared for cells suspended in phosphate-buffered saline (PBS) or PBS supplemented with 2 O/ O nutrient broth. Addition of 2% nutrient broth to cells suspended in PBS yielded minimal growth and did not significantly change the mean zeta potential of the organisms, which was around -13 mV. However, a comparatively larger proportion of the organisms had more negative zeta potentials in the presence of nutrient broth. This change was concurrent with a slight decrease in cellsurface hydrophobicity, as measured by water contact angles, from 119" to around 112". The initial deposition rate of P. aemginosa AKI to silicone rubber, as studied in a parallel plate flow Chamber, increased from 344 cmm2 s-' in the absence of nutrient broth to 505 cm' 2 s'l in its presence. No stationary level of adhesion was observed in the presence of nutrient broth, instead the number of adhering cells increased steadily at a rate of approximately 85 ern+ s'l.Fluorescent staining of adhering cells demonstrated that for adhesion from buffer only 2% of the adhering cells were metabolically active, whereas in case of deposition from PBS supplemented with nutrient broth, 67% of the adhering cells were metabolically active. It is concluded that the deposition rates measured in the parallel plate flow chamber with 2% nutrient broth added to the PBS suspension represent an interplay of adhesion and surfaceassociated growth.

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Physicochemical Aspects of Deposition of Streptococcus thermophilus B to Hydrophobic and Hydrophilic Substrata in a Parallel Plate Flow Chamber

Cited by 44 publications

References 0 publications

Bacterial adhesion to hydroxyl- and methyl-terminated alkanethiol self-assembled monolayers

Bacterial adhesion to hydroxyl- and methyl-terminated alkanethiol self-assembled monolayers

Decreased <em>Pseudomonas aeruginosa</em> biofilm formation on nanomodified endotracheal tubes: a dynamic lung model

Adhesion of Pseudomonas aeruginosa to silicone rubber in a parallel plate flow chambe in the absence and presence of nutrient broth

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