Physicochemical and in-vitro biological analysis of bio-functionalized titanium samples in a protein-rich medium

Rao, Shradha; Astaneh, Sarah Hashemi; Rivera, José María; Silva, Filipe; Takoudis, Christos G.; Bijukumar, Divya; Souza, Júlio C. M.; Mathew, Mathew T.

doi:10.1016/j.jmbbm.2019.03.019

Cited by 15 publications

(12 citation statements)

References 36 publications

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“…There were no differences in the adherence of S. aureus to a titanium surface pre-conditioned with fibrinogen when compared to the non-conditioned surface ( Figure S2). These results are in line with the ones obtained by Rao et al [52] that showed how the pre-conditioning of titanium with fibrinogen and fibrinogen clotted with thrombin promoted osseointegration, but the biofilm formation by Enterococcus faecalis in these bio-functionalized materials did not show significant differences with the titanium control.…”

Section: Discussionsupporting

confidence: 92%

Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and SaOS-2 Cells

et al. 2020

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Biofilm-mediated infection is a major cause of bone prosthesis failure. The lack of molecules able to act in biofilms has driven research aimed at identifying new anti-biofilm agents via chemical screens. However, to be able to accommodate a large number of compounds, the testing conditions of these screenings end up being typically far from the clinical scenario. In this study, we assess the potential applicability of three previously discovered anti-biofilm compounds to be part of implanted medical devices by testing them on in vitro systems that more closely resemble the clinical scenario. To that end, we used a competition model based on the co-culture of SaOS-2 mammalian cells and Staphylococcus aureus (collection and clinical strains) on a titanium surface, as well as titanium pre-conditioned with high serum protein concentration. Additionally, we studied whether these compounds enhance the previously proven protective effect of pre-incubating titanium with SaOS-2 cells. Out of the three, DHA1 was the one with the highest potential, showing a preventive effect on bacterial adherence in all tested conditions, making it the most promising agent for incorporation into bone implants. This study emphasizes and demonstrates the importance of using meaningful experimental models, where potential antimicrobials ought to be tested for the protection of biomaterials in translational applications.

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Section: Discussionsupporting

confidence: 92%

Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and SaOS-2 Cells

et al. 2020

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“…Moreover, all the three trials (trial 16–18) in MWCNTs mixed dielectric at a discharge current of 4A exhibits good roughness values within the variation of 4% (approx.). The roughness value reported is acceptable and within the range, required for the bio-implants for proper cell proliferation, bone-implant fixation [ 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

Enhancing Corrosion and Wear Resistance of Ti6Al4V Alloy Using CNTs Mixed Electro-Discharge Process

Singh¹,

Ablyaz

Шлыков

et al. 2020

Micromachines

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This paper presents wear and corrosion resistance analysis of carbon nanotubes coated with Ti-6Al-4V alloy processed by electro-discharge treatment. The reported work is carried out using Taguchi’s L18 orthogonal array to design the experimental matrix by varying five input process parameters i.e., dielectric medium (plain dielectric, multi-walled carbon nanotubes (MWCNTs) mixed dielectric), current (1–4 A), pulse-on-time (30–60 µs), pulse-off-time (60–120 µs), and voltage (30–50 V). The output responses are assessed in terms of microhardness and surface roughness of the treated specimen. X-ray diffraction (XRD) spectra of the coated sample reveal the formation of intermetallic compounds, oxides, and carbides, whereas surface morphology is observed using scanning electron microscopy (SEM) analysis. For the purpose of the in-vitro wear behavior of treated samples, the surface with superior microhardness values in plain dielectric and MWCNTs mixed dielectric is compared using a pin-on-disc type wear test. Furthermore, electrochemical corrosion test is also conducted to portray the dominance of treated substrate of Ti-6Al-4V alloy for biomedical applications. It is concluded that the wear-resistant and the corrosion protection efficiency of the MWCNTs treated substrate enhanced to 95%, and 96.63%, respectively.

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“…Studies have proposed approaches to enhance implant osseointegration by developing an osteogenic surface or to prevent infection by incorporating antibiotic molecules or peptides in the surface coating (Smeets et al 2016; Escobar et al 2021). One promising alternative could be the development of implants with multifunctional surfaces capable of interacting with the host tissue to increase osseointegration while reducing bacterial activity (Raphel et al 2016; Rao et al 2019). Implant surfaces can be modified with bioactive therapeutics such as BMP-2 to enhance osteogenic potential; however, high costs and complications at high dosages hinder translation into clinical applications.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and Osteoinductive Implant Surface Using Layer-by-Layer Assembly

et al. 2021

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Osseointegration of dental, craniofacial, and orthopedic implants is critical for their long-term success. Multifunctional surface treatment of implants was found to significantly improve cell adhesion and induce osteogenic differentiation of dental-derived stem cells in vitro. Moreover, local and sustained release of antibiotics via nanolayers from the surface of implants can present unparalleled therapeutic benefits in implant dentistry. Here, we present a layer-by-layer surface treatment of titanium implants capable of incorporating BMP-2–mimicking short peptides and gentamicin to improve their osseointegration and antibacterial features. Additionally, instead of conventional surface treatments, we employed polydopamine coating before layer-by-layer assembly to initiate the formation of the nanolayers on rough titanium surfaces. Cytocompatibility analysis demonstrated that modifying the titanium implant surface with layer-by-layer assembly did not have adverse effects on cellular viability. The implemented nanoscale coating provided sustained release of osteoinductive peptides with an antibacterial drug. The surface-functionalized implants showed successful osteogenic differentiation of periodontal ligament stem cells and antimicrobial activity in vitro and increased osseointegration in a rodent animal model 4 wk postsurgery as compared with untreated implants. Altogether, our in vitro and in vivo studies suggest that this approach can be extended to other dental and orthopedic implants since this surface functionalization showed improved osseointegration and an enhanced success rate.

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Physicochemical and in-vitro biological analysis of bio-functionalized titanium samples in a protein-rich medium

Cited by 15 publications

References 36 publications

Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and SaOS-2 Cells

Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and SaOS-2 Cells

Enhancing Corrosion and Wear Resistance of Ti6Al4V Alloy Using CNTs Mixed Electro-Discharge Process

Antibacterial and Osteoinductive Implant Surface Using Layer-by-Layer Assembly

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