Physicochemical and biological study of selected hydrophobic polyethylenimine-based polycationic liposomes and their complexes with DNA

Masotti, Andrea; Moretti, Fabiola; Mancini, Francesca; Russo, Giuseppina; Lauro, Nicoletta Di; Checchia, Paola; Marianecci, Carlotta; Carafa, Maria; Santucci, E.; Ortaggi, Giancarlo

doi:10.1016/j.bmc.2006.10.066

Cited by 61 publications

(55 citation statements)

References 20 publications

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“…We have observed previously that the minor groove binding by polyamine polymers prevents, at least in part if not completely, the fluorescence quenching of the intercalating dye. 43 The tighter interaction of PEI and PEICNTs with DNA compared to PAMAM was also confirmed by the heparin competition assay. 44 This assay consists in the incubation of polymer-coated CNTs complexed with oligonucleotides with a negatively charged high-molecular weight macromolecule (ie, heparin), which could electrostatically compete with the oligonucleotide and displace it ( Figure 3B).…”

Section: Gel Retardation Assaysmentioning

confidence: 75%

“…Both PEI and PAMAM polymers formed large aggregates with the miRNA that were unable to migrate into the gel, in agreement with data already reported. 21,43 Similarly, the complete formation of PEI-CNTs and PAMAM-CNTs complexes occurred at a higher weight ratio of 10:1 (lane 8) and 20:1 (lane 16), respectively. The higher amount of PAMCNTs needed to bind completely the oligo suggests that this compound could bind the nucleic acid less tightly.…”

Section: Gel Retardation Assaysmentioning

confidence: 93%

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Biophysical and biological contributions of polyamine-coated carbon nanotubes and bidimensional buckypapers in the delivery of miRNAs to human cells

Celluzzi

Paolini

D’Oria

et al. 2017

IJN

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Recent findings in nanomedicine have revealed that carbon nanotubes (CNTs) can be used as potential drug carriers, therapeutic agents and diagnostics tools. Moreover, due to their ability to cross cellular membranes, their nanosize dimension, high surface area and relatively good biocompatibility, CNTs have also been employed as a novel gene delivery vector system. In our previous work, we functionalized CNTs with two polyamine polymers, polyethyleneimine (PEI) and polyamidoamine dendrimer (PAMAM). These compounds have low cytotoxicity, ability to conjugate microRNAs (such as miR-503) and, at the same time, transfect efficiently endothelial cells. The parameters contributing to the good efficiency of transfection that we observed were not investigated in detail. In fact, the diameter and length of CNTs are important parameters to be taken into account when evaluating the effects on drug delivery efficiency. In order to investigate the biophysical and biological contributions of polymer-coated CNTs in delivery of miRNAs to human cells, we decided to investigate three different preparations, characterized by different dimensions and aspect ratios. In particular, we took into account very small CNTs, a suspension of CNTs starting from the commercial product and a 2D material based on CNTs (ie, buckypapers [BPs]) to examine the transfection efficiency of a rigid scaffold. In conclusion, we extensively investigated the biophysical and biological contributions of polyamine-coated CNTs and bidimensional BPs in the delivery of miRNAs to human cells, in order to optimize the transfection efficiency of these compounds to be employed as efficient drug delivery vectors in biomedical applications.

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Section: Gel Retardation Assaysmentioning

confidence: 75%

Section: Gel Retardation Assaysmentioning

confidence: 93%

Biophysical and biological contributions of polyamine-coated carbon nanotubes and bidimensional buckypapers in the delivery of miRNAs to human cells

Celluzzi

Paolini

D’Oria

et al. 2017

IJN

Self Cite

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“…It was reported that alkylated bPEI25K could assemble into a liposome-like structure at pH 7.0, and this "polycationic liposomes" phenomenon is thought to be common for hydrophobic derivatives of bPEI25K. 28 Due to the huge difference in the chain length between bPEI25K and hydrocarbons, it might be impossible for alkylated bPEI25K to form a micelle-like structure. But in the case of LMW PEI (such as bPEI1800), whose chain length is comparable to that of medium-chain fatty acids and hydrocarbons, the micelle-like assembly of bPEI1800-C 12 might occur more easily.…”

Section: Discussionmentioning

confidence: 99%

“…bPEI1800-C 12 / DNA polyplexes were found to be quite robust against anion displacement ( Figure 3A) and exhibited high in vitro transfection efficiency in the presence of serum ( Figure 5). The molecular formula of bPEI1800-C 12 is (C 2 H 4 N) 42 (C 12 H 25 ) 42 n, where n is the C 12 substitution degree, 28 so it was thought there would be 8.3 C 12 chains in every molecule of bPEI1800-C 12 -19.72. It has been reported that cationic surfactant with more than one protonation center and hydrophobic chain showed much higher stability than their corresponding monovalent (single hydrophobic chain and single head group).…”

Section: Discussionmentioning

confidence: 99%

“…28 Briefly, 1-bromododecane was reacted with a solution of bPEI1800 (1 g, 0.56 mmol) in dichloromethane (150 mL) at room temperature (RT) for 24 hours. The resulting solvent was concentrated under reduced pressure and further dialyzed, first against an ethanol/ water (1:1) solution (4 L five times), then against doubledistilled water for 1 day.…”

Section: Synthesis Of Bpei1800-c 12 Derivativesmentioning

confidence: 99%

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High gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect

Liu¹,

Huang²,

Jin³

et al. 2014

IJN

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To our knowledge, the mechanism underlying the high transfection efficiency of alkylated low-molecular-weight polyethylenimine (PEI) is not yet well understood. In this work, we grafted branched PEI (molecular weight of 1,800 Da; bPEI1800) with lauryl chains (C 12 ), and found that bPEI1800-C 12 was structurally similar to gemini surfactant and could similarly assemble into micelle-like particles. Stability, cellular uptake, and lysosome escape ability of bPEI1800-C 12 /DNA polyplexes were all greatly enhanced after C 12 grafting. bPEI1800-C 12 /DNA polyplexes exhibited significantly higher transfection efficiency than Lipofectamine™ 2000 in the presence of serum. Bioluminescence imaging showed that systemic injection of bPEI1800-C 12 /DNA polyplexes resulted in intensive luciferase expression in vivo and bioluminescence signals that could be detected even in the head. Altogether, the high transfection efficacy of bPEI1800-C 12 was because bPEI1800-C 12 , being an analog of gemini surfactant, facilitated lysosome escape and induced the coil–globule transition of DNA to assemble into a highly organized micelle-like structure that showed high stability.

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Katari¹,

Shunmugaperumal²,

Rahman³

2020

Oil‐in‐Water Nanosized Emulsions for Drug Delivery and Targeting

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Physicochemical and biological study of selected hydrophobic polyethylenimine-based polycationic liposomes and their complexes with DNA

Cited by 61 publications

References 20 publications

Biophysical and biological contributions of polyamine-coated carbon nanotubes and bidimensional buckypapers in the delivery of miRNAs to human cells

Biophysical and biological contributions of polyamine-coated carbon nanotubes and bidimensional buckypapers in the delivery of miRNAs to human cells

High gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect

Selected Case Studies

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