Physico-Chemical Properties of Inorganic NPs Influence the Absorption Rate of Aquatic Mosses Reducing Cytotoxicity on Intestinal Epithelial Barrier Model

Matteis, Valeria De; Rojas, Makarena; Cascione, Mariafrancesca; Mazzotta, Stefano; Sansebastiano, Gian Pietro Di; Rinaldi, R.

doi:10.3390/molecules26102885

Cited by 5 publications

(3 citation statements)

References 60 publications

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“…The results obtained were in line with those reported by other authors for the human tumor Caco-2 colon adenocarcinoma cell line [ 94 , 95 , 96 ]. A study conducted by Gomaa [ 15 ] on the colon carcinoma cell line showed toxicity at much lower concentrations, the IC50 value of the tested AgNPs had a range between 2.3 and 2.2 µg/mL.…”

Section: Discussionsupporting

confidence: 92%

Antimicrobial and Antiproliferative Activity of Green Synthesized Silver Nanoparticles Using Bee Bread Extracts

Urcan,

Criste,

Szanto

et al. 2023

Pharmaceutics

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Bee bread (BB) is a fermented mixture of bee pollen, is rich in proteins, amino acids, fatty acids, polyphenols, flavonoids, as well as other bioactive compounds, and is considered functional food for humans. In this study, we explored an innovative green synthesis of colloidal silver nanoparticles, using BB extracts as reducing and stabilizing agents. A preliminary chemical characterization of the BB extracts was conducted. The plasmonic response of the as-synthesized silver nanoparticles (BB-AgNPs) was evaluated by UV–Vis spectroscopy, while their hydrodynamic diameter and zeta potential were investigated by dynamic light spectroscopy (DLS). Transmission electron microscopy (TEM) analysis pointed out polydisperse NPs with quasi-spherical shapes. The newly synthesized nanoparticles showed good antioxidant activity against the tested free radicals, DPPH, ABTS•+, and FRAP, the best results being obtained in the case of ABTS•+. BB-AgNPs exhibited good antibacterial activity on the tested Gram-positive and Gram-negative bacterial strains: herein S. aureus, B. cereus, E. faecalis, E. coli, P. aeruginosa, S. enteritidis, and on yeast C. albicans, respectively. The inhibition diameters varied between 7.67 ± 0.59 and 22.21 ± 1.06 mm, while the values obtained for minimum inhibitory concentration varied between 0.39 and 6.25 µg/mL. In vitro antiproliferative activity was tested on colon adenocarcinoma, ATCC HTB-37 cell line, and the results have shown that the green synthetized BB-AgNPs induced a substantial decrease in tumor cell viability in a dose-dependent manner with an IC50 ranging from 24.58 to 67.91 µg/mL. Consequently, more investigation is required to comprehend the processes of the cytotoxicity of AgNPs and develop strategies to mitigate their potentially harmful effects while harnessing their antimicrobial properties.

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Section: Discussionsupporting

confidence: 92%

Antimicrobial and Antiproliferative Activity of Green Synthesized Silver Nanoparticles Using Bee Bread Extracts

Urcan,

Criste,

Szanto

et al. 2023

Pharmaceutics

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“…We used an OSMOMAT 070 osmometer for the measurement of osmolality of our solutions as vapor pressure method is appropriate for determination of osmolality or molecular weight [22][23][24][25]. In vitro cytotoxicity experiments were carried out on Caco-2 human colorectal adenocarcinoma cells as a general model cell line, but also as indicators of the susceptibility of the gastrointestinal tract as they morphologically represent the intestinal epithelium [26,27]. The cytotoxicity was assessed by MTT and Neutral Red assays which are widely used in vitro techniques.…”

Section: Discussionmentioning

confidence: 99%

Comparative Investigation of Cellular Effects of Polyethylene Glycol (PEG) Derivatives

et al. 2022

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Nowadays, polyethylene glycols referred to as PEGs are widely used in cosmetics, consumer care products, and the pharmaceutical industry. Their advantageous properties such as chemical stability, low immunogenicity, and high tolerability explain why PEGs are applied in many fields of pharmaceutical formulations including parenteral, topical, ophthalmic, oral, and rectal preparations and also in modern drug delivery systems. Given their extensive use, they are considered a well-known group of chemicals. However, the number of large-scale comparative studies involving multiple PEGs of wide molecular weight range is low, as in most cases biological effects are estimated upon molecular weight. The aim of this publication was to study the action of PEGs on Caco-2 cells and G. mellonella larvae and to calculate the correlation of these effects with molecular weight and osmolality. Eleven PEGs of different molecular weight were used in our experiments: PEG 200, PEG 300, PEG 400, PEG 600, PEG 1000, PEG 1500, PEG 4000, PEG 8000, PEG 10,000, 12,000, and PEG 20,000. The investigated cellular effects included cytotoxicity (MTT and Neutral Red assays, flow cytometry with propidium iodide and annexin V) and autophagy. The osmolality of different molecular weight PEGs with various concentrations was measured by a vapor pressure osmometer OSMOMAT 070 and G. mellonella larvae were injected with the solutions of PEGs. Sorbitol was used as controls of the same osmolality. Statistical correlation was calculated to describe the average molecular weight dependence of the different measured effects. Osmolality, the cytotoxicity assays, flow cytometry data, and larvae mortality had significant correlation with the structure of the PEGs, while autophagosome formation and the proportion of early apoptotic cells showed no statistical correlation. Overall, it must be noted that PEGs must be tested individually for biological effects as not all effects can be estimated by the average molecular weight.

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“…Due to their high surface-to-volume ratio, nanosized structures exhibit different physicochemical properties compared to bulk structures [91]. Consequently, NPs demonstrate greater activity in various biological applications [92].…”

Section: Plant-extract-based Npsmentioning

confidence: 99%

Reports of Plant-Derived Nanoparticles for Prostate Cancer Therapy

Elbagory

Hull

Meyer

et al. 2023

Plants

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Plants have demonstrated potential in providing various types of phytomedicines with chemopreventive properties that can combat prostate cancer. However, despite their promising in vitro activity, the incorporation of these phytochemicals into the market as anticancer agents has been hindered by their poor bioavailability, mainly due to their inadequate aqueous solubility, chemical instability, and unsatisfactory circulation time. To overcome these drawbacks, it has been suggested that the incorporation of phytochemicals as nanoparticles can offer a solution. The use of plant-based chemicals can also improve the biocompatibility of the formulated nanoparticles by avoiding the use of certain hazardous chemicals in the synthesis, leading to decreased toxicity in vivo. Moreover, in some cases, phytochemicals can act as targeting agents to tumour sites. This review will focus on and summarize the following points: the different types of nanoparticles that contain individual phytochemicals or plant extracts in their design with the aim of improving the bioavailability of the phytochemicals; the therapeutic evaluation of these nanoparticles against prostate cancer both in vitro and in vivo and the reported mode of action and the different types of anticancer experiments used; how the phytochemicals can also improve the targeting effects of these nanoparticles in some instances; and the potential toxicity of these nanoparticles.

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Physico-Chemical Properties of Inorganic NPs Influence the Absorption Rate of Aquatic Mosses Reducing Cytotoxicity on Intestinal Epithelial Barrier Model

Cited by 5 publications

References 60 publications

Antimicrobial and Antiproliferative Activity of Green Synthesized Silver Nanoparticles Using Bee Bread Extracts

Antimicrobial and Antiproliferative Activity of Green Synthesized Silver Nanoparticles Using Bee Bread Extracts

Comparative Investigation of Cellular Effects of Polyethylene Glycol (PEG) Derivatives

Reports of Plant-Derived Nanoparticles for Prostate Cancer Therapy

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