Beer antioxidants
originate mainly from malts, classified as colored,
caramel, and roasted, according to the malting process. This study
aimed to characterize, in terms of phenolic antioxidants, three types
of Pale Ale craft beers brewed using increasing percentage of dark
malt (0, 5, and 15% Caraamber malt, called PA100, PA95, PA85, respectively)
and to evaluate the impact of dealcoholization by osmotic distillation
(OD) on the same antioxidants. All the alcoholic (PA, 6.2–6.8
vol %) and low alcoholic (LA-PA, 1 vol %) beers were analyzed by HPLC-ESI-MS/MS,
total phenolic content (TPC), and antioxidant activity (AA): similar
phenolic profiles were evidenced and 43 compounds identified or tentatively
identified. Some differences were found among PA100, PA95, and PA85:
PA85 was richer in free phenolic compounds (10.55 mg/L) and had a
higher TPC (463.7 GAE mg/L) and AA (852.1 TE mg/L). LA-PA beers showed
the same phenolic profile and similar TPC and AA compared to PA beers;
however, there were some differences regarding LA-PA85 (5.91 mg/L).
Dealcoholization by OD seemed to weakly affect the phenolic fraction.
ESI-MS/MS infusion experiments evidenced oligosaccharides, small organic
acids, and amino acids, whose presence was confirmed and quantitated
by NMR: besides ethanol and other alcohols, weak to strong loss of
low-molecular-weight metabolites was evidenced in LA-PA beers.