Physico-chemical (GC-MS) measurements of juvenile hormone III titres during embryogenesis of<i>Locusta migratoria</i>

Temin, Gina; Zander, M.; Roussel, J.‐P.

doi:10.1080/01688170.1986.10510184

Cited by 41 publications

(31 citation statements)

References 13 publications

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“…sanguinipes (Couche et al, 1985) and other Acrididae (Bergot et al, 1981;Mauchamp et al, 1985;Dale and Tobe, 1986;Temin et al, 1986). In L. migratoria the JH carrier protein shows a significant preference for JH I11 over other forms of the hormone (Peter et al, 1979;Roberts, 1985).…”

Section: Discussionmentioning

confidence: 98%

Identification, characterization, and developmental profile of a high molecular weight, juvenile hormone‐binding protein in the hemolymph of the migratory grasshopper, Melanoplus sanguinipes

Ismail

Gillott

1995

Arch Insect Biochem Physiol

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In the hemolymph of Melanoplus sanguinipes, a high molecular weight juvenile hormone binding protein (JHBP) was identified by photoaffinity labelling and found to have a M, of 480,000. The JHBF', purified using native gel electrophoresis followed by electroelution, has an equilibrium dissociation constant for JH Ill of 2.1 n M and preferentially binds JH Ill over JH I. Antibody raised against JHBP recognized only the 480,000 band. Under denaturing conditions the native JHBP gave a single band with a M, 78,000. The antibody against native JHBP recognized only the 78,000 protein in SDS-treated hemolymph samples, indicating that JHBP is a hexamer in this species. The concentration of JHBP fluctuates in both the sexes during nymphal and adult development in parallel with total protein content of hemolymph. o 1995 Wiiey-Liss, Inc.

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Section: Discussionmentioning

confidence: 98%

Identification, characterization, and developmental profile of a high molecular weight, juvenile hormone‐binding protein in the hemolymph of the migratory grasshopper, Melanoplus sanguinipes

Ismail

Gillott

1995

Arch Insect Biochem Physiol

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“…In the cockroach Nuuphoeta JH was absent in newly formed eggs 1311 but later appeared and rose to high levels (960 ng JH III/g) at the stages of 1st larval cuticle formation and eye pigmentation [ll]; in this species we observed very high quantities of methyl farnesoate concomitant with the increase in JH 111 [8]. In Locusta, freshly laid eggs contained 1.8 ng/g JH 111 and embryos at the stage of 1st cuticle formation 8 ng/g JH I11 [9]. It would seem that the composition and content of JH in embryos varies considerably among different species.…”

Section: Qualitative and Quantitative Variations In Jh From The Egg Tmentioning

confidence: 91%

“…The titer of JH I1 fluctuated rapidly between 1 and 4 ng/ml in the feeding 4th-instar larvae and reached its highest level (9 ng/ml) in the late scotophase, concomitant with the peak in 20E, 12 h before ecdysis into the 5th instar. Thereafter the JH titer dropped rapidly and then rose again during the scotophase after ecdysis.…”

Section: Ecdysteroids and Jh In Hemolymph Of Penultimate And Last-insmentioning

confidence: 97%

Qualitative and quantitative analyses of juvenile hormone and ecdysteroids from the egg to the pupal molt in Trichoplusia ni

Grossniklaus-Bürgin

Lanzrein

1990

Arch Insect Biochem Physiol

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A method was developed to determine in the same extract juvenile hormone and various types of ecdysteroids in precisely staged eggs and larvae of Trichoplusia ni. Ecdysteroids were tentatively identified on the basis of their retention time in ion suppression reversed-phase HPLC and their cross-reactivity with two relatively non-specific, complimentary antibodies, whereas juvenile hormone was identified using reversed-phase HPLC combined with Galleria bioassay. Freshly laid eggs contained low levels of immunoreactive ecdysteroids. Mid-polar ecdysteroids increased in the phase of segmentation (14-18 h) and 1st larval cuticle formation (36-44 h), when 20-hydroxyecdysone and 20,26-dihydroxyecdysone were found to be predominant. Only traces of ecdysone and 26-hydroxyecdysone were seen. Toward hatching ecdysteroids decreased and represented mainly compounds more polar than 20,26-dihydroxyecdysone. In larval development ecdysteroids were low at the beginning of the feeding phases, increased toward cessation of feeding, and reached highest levels 12-15 h before ecdysis. In feeding stages ecdysone and 20-hydroxyecdysone were predominant, whereas in molting stages they were seen together with 20,26-dihydroxyecdysone and 20-hydroxyecdysonoic acid. The juvenile hormone titer was very low in freshly laid eggs and was high (approximately 25 ng/g) in embryos at the stage of 1st larval cuticle formation and eye pigmentation. In eggs we tentatively identified juvenile hormones I and II, whereas in larval stages juvenile hormone II appeared to be the predominant or exclusive juvenile hormone. Its titer fluctuated rapidly and was high in early 1st-instar larvae and again before the molts into the 3rd, 4th, and 5th instar. Highest titers were reached concomitant with the peak in 20-hydroxyecdysone 12-15 h before ecdysis.

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“…During embryogenesis there are four peaks of ecdysone and its metabolites that correlate with deposition of the three embryonic cuticles as well as the serosal "cuticle", a thin layer secreted by the serosal cells that underlie the chorion (Lagueux et al 1979). A peak of JH III appears at about 70% of embryogenesis, coinciding with terminal differentiation and persisting through the production of the first nymphal cuticle (Temin 1986). …”

Section: Embryonic Development and Cuticle Formation In Orthopteramentioning

confidence: 95%

“…In Orthoptera, these three cuticles coincide with three peaks of ecdysteroids (Lagueux et al 1979). JH appears at about 70% of embryonic development and is present during the formation of the last cuticle, the first instar nymphal cuticle (Temin et al 1986). Treatment of Schistocerca gregaria embryos with JH mimics prior to this endogenous peak causes both morphological and growth defects (Novak 1969), and nymphal features on the second embryonic (pronymphal) cuticle (Sbrenna-Micciarelli 1977).…”

Section: Introductionmentioning

confidence: 97%

Juvenile hormone acts at embryonic molts and induces the nymphal cuticle in the direct-developing cricket

2004

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During embryogenesis of hemimetabolous insects, the sesquiterpenoid hormone, juvenile hormone (JH), appears late in embryogenesis coincident with formation of the first nymphal cuticle. We tested the role of embryonic JH by treating cricket embryos with JH III, or the JH-mimic (JHM) pyriproxifen, during early embryogenesis. We found two discrete windows of JH sensitivity. The first occurs during the formation of the first (E1) embryonic cuticle. Treatment with JHM prior to this molt produced small embryos that failed to complete the movements of katatrepsis. Embryos treated after the E1 molt but before the second embryonic (pronymphal) molt completed katatrepsis but then failed to complete dorsal closure and precociously formed nymphal, rather than pronymphal characters. This second sensitivity window was further assessed by treating embryos with low doses of JH III prior to the pronymphal molt. With low doses, mosaic cuticles were formed, bearing features of both the pronymphal and nymphal stages. The nymphal characters varied in their sensitivity to JH III, due at least in part to differences in the timing of their sensitivity windows. Unexpectedly, many of the JH III-treated embryos with mosaic and precocious nymphal cuticles made a second nymphal cuticle and successfully hatched. JH treatment also affected the growth of the embryos. By focusing on the developing limb, we found that the effect of JH upon growth was asymmetric, with distal segments more affected than proximal ones, but this was not reflected in misexpression of Distal-less or Bric-a-brac, which are involved in proximal-distal patterning of the limb.

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Physico-chemical (GC-MS) measurements of juvenile hormone III titres during embryogenesis ofLocusta migratoria

Cited by 41 publications

References 13 publications

Identification, characterization, and developmental profile of a high molecular weight, juvenile hormone‐binding protein in the hemolymph of the migratory grasshopper, Melanoplus sanguinipes

Identification, characterization, and developmental profile of a high molecular weight, juvenile hormone‐binding protein in the hemolymph of the migratory grasshopper, Melanoplus sanguinipes

Qualitative and quantitative analyses of juvenile hormone and ecdysteroids from the egg to the pupal molt in Trichoplusia ni

Juvenile hormone acts at embryonic molts and induces the nymphal cuticle in the direct-developing cricket

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