Physico-chemical factors influencing the shikonin derivatives production in cell suspension cultures ofArnebia euchroma(Royle) Johnston, a medicinally important plant species

Abstract

Cell suspension cultures of Arnebia euchroma were raised from in vitro leaf-derived friable callus on liquid MS [Murashige and Skoog] medium supplemented with BAP (6-benzylaminopurine) (10.0 μM) and IBA (indole-3-butyric acid) (5.0 μM). A two-stage culture system was employed using growth and production medium for cell biomass and shikonin derivatives, respectively. Factors such as light, temperature, sucrose and pH (hydrogen ion concentration) were studied to observe their effect on the shikonin derivative production. Light conditions completely inhibited shikonin derivative production. Out of different temperature regimes tested, the highest yield (586.17 μg/g FW) was found at 25°C. Maximum production (656.14 μg/g FW) was observed in 6% sucrose. An alkaline pH (7.25-9.50) favoured shikonin derivative production. The results showed that physical and chemical factors greatly influence the production of shikonin derivatives in cell suspension cultures of A. euchroma. Therefore, by employing optimum culture conditions, it is possible to enhance the production of secondary compounds from the cells. The factors optimized for in vitro production of shikonin derivatives during the present study can successfully be employed for their large-scale production in bioreactors.

Enhanced production of napthoquinone metabolite (shikonin) from cell suspension culture of Arnebia sp. and its up-scaling through bioreactor

“…The shikonin derivatives in the organic phase obtained being recovered as a blue aqueous solution by using 1 ml of 2.5 % KOH. Absorbance of samples was recorded at 620 nm using spectrophotometer (Hussain et al 2012, 2011). …”

“…Owing to these properties, genus Arnebia is over-exploited and therefore, placed under the category of critically endangered species. The plants are very difficult to cultivate through conventional agriculture (possess third level of difficulty in seed germination) (Malik et al 2011). …”

Cyanobacteria, Lyngbya aestuarii and Aphanothece bullosa as antifungal and antileishmanial drug resources

“…Secondary metabolites produced from cyanobacteria are found to be antibacterial, antifungal, antialgal, anticancerous and antimalarial with many more activitys [3,4] . Microbial productions of metabolites are greatly influenced by factors such as pH, temperature, and light intensity [5][6][7][8] . However, species specificity of secondary metabolites is well established [9] .…”

Screening and kinetic studies of catharanthine and ajmalicine accumulation and their correlation with growth biomass inCatharanthus roseushairy roots

“…Recently, it has been shown that vinblastine and vincristine can be semi-synthesized by using catharanthine and vindoline as precursors (Ishikawa et al 2009). For several years, plant tissue culture has been considered as a promising biotechnological approach to improve the secondary metabolites production (Malik et al 2011(Malik et al , 2014. Organized cultures, especially transgenic hairy root cultures induced by Agrobacterium rhizogenes, represent a reliable alternative to undifferentiated tissue cultures, such as cell suspensions, for the production of valuable secondary metabolites (Mujib et al 2012;Malik et al 2016).…”