Physico-chemical characterization of single bacteria and spores using optical tweezers

Nilsson, Daniel P G; Jonsmoen, Unni Lise; Malyshev, Dmitry; Öberg, Rasmus; Wiklund, Krister; Andersson, Magnus

doi:10.1016/j.resmic.2023.104060

Cited by 4 publications

(3 citation statements)

References 71 publications

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Section: Isolation Of Bacteriamentioning

confidence: 99%

“…If a cell is nearby, it interacts with the laser and, due to the transfer of momentum from the incident photons, is drawn into the trap by gradient optical forces. The trapped cell may be moved in three dimensions by either moving the focal point or translating the sample chamber while maintaining the trap fixed [243,[249][250][251]. Therefore, this method is an effective tool for the sorting of cells, especially as a strategy for the selective isolation of microbial cells from a diverse population [6,249,250].…”

Section: Sample Processing: Isolation From Matrix and Isolation Strat...mentioning

confidence: 99%

“…The trapped cell may be moved in three dimensions by either moving the focal point or translating the sample chamber while maintaining the trap fixed [243,[249][250][251]. Therefore, this method is an effective tool for the sorting of cells, especially as a strategy for the selective isolation of microbial cells from a diverse population [6,249,250]. When combined with Raman spectroscopy, this approach is useful for investigating microbial communities and identifying cells of interest, which is valuable for studying unculturable and unknown species [244].…”

Section: Sample Processing: Isolation From Matrix and Isolation Strat...mentioning

confidence: 99%

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Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms

Frempong,

Salbreiter,

Mostafapour

et al. 2024

Molecules

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Raman spectroscopy is an emerging method for the identification of bacteria. Nevertheless, a lot of different parameters need to be considered to establish a reliable database capable of identifying real-world samples such as medical or environmental probes. In this review, the establishment of such reliable databases with the proper design in microbiological Raman studies is demonstrated, shining a light into all the parts that require attention. Aspects such as the strain selection, sample preparation and isolation requirements, the phenotypic influence, measurement strategies, as well as the statistical approaches for discrimination of bacteria, are presented. Furthermore, the influence of these aspects on spectra quality, result accuracy, and read-out are discussed. The aim of this review is to serve as a guide for the design of microbiological Raman studies that can support the establishment of this method in different fields.

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Section: Isolation Of Bacteriamentioning

confidence: 99%

Section: Sample Processing: Isolation From Matrix and Isolation Strat...mentioning

confidence: 99%

Section: Sample Processing: Isolation From Matrix and Isolation Strat...mentioning

confidence: 99%

See 1 more Smart Citation

Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms

Frempong,

Salbreiter,

Mostafapour

et al. 2024

Molecules

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show abstract

Insights into the Bacillus anthracis, cereus and thuringiensis world through the BACT conference

Broussolle

Gohar

Slamti

et al. 2023

Research in Microbiology

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Femtosecond laser-assisted selective holding with ultra-low power for direct manipulation of biological species

Krishna,

Burrow,

Jiang

et al. 2024

J. Biomed. Opt.

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Optical tweezers (OTs) have emerged as an essential technique for manipulating nanoscopic particulates and biological specimens with sub-micron precision and have revolutionized various fields, including biology and colloidal physics. However, traditional optical trapping techniques often rely on moderate-to highpower continuous wave (CW) lasers, which can introduce unwanted thermal effects and photodamage to delicate samples. An innovative alternative has emerged through the utilization of femtosecond (fs) lasers at ultra-low average powers on the order of tens of microwatt. Unexpectedly overlooked until now, this method enables the direct trapping and manipulation of cells without relying on functionalized spheres.Aim: We aim to compare the trap stiffness of CW and fs lasers in an unexplored average power regime (sub-1 mW) on cells within the intermediate-size regime.Approach: A CW or fs laser is used to trap cells in an inverted microscope setup. We trap five different pathogenic bacteria with different morphologies to compare trap stiffness.Results: We find that fs laser-assisted selective holding with ultra-low power (FLASH-UP) exhibits five times greater trap stiffness than CW-based OTs and can trap at lower intensities. Furthermore, we demonstrate that FLASH-UP does not impact cell motility.Conclusions: FLASH-UP displays higher trap stiffness at average powers below 1 mW and does not impact cell functionality. These results pave the way for ultralow-power trapping of cells for applications in sorting, bio-sensing, in vivo cell manipulation, and single-cell analysis.

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Physico-chemical characterization of single bacteria and spores using optical tweezers

Cited by 4 publications

References 71 publications

Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms

Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms

Insights into the Bacillus anthracis, cereus and thuringiensis world through the BACT conference

Femtosecond laser-assisted selective holding with ultra-low power for direct manipulation of biological species

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