Physico-chemical characterization of asolectin–genistein liposomal system: An approach to analyze its in vitro antioxidant potential and effect in glioma cells viability

Abstract

In this study, the interaction between soy isoflavone genistein and asolectin liposomes was investigated by monitoring the effects of isoflavone on lipidic hydration, mobility, location and order. These properties were analyzed by the following techniques: horizontal attenuated total reflection Fourier transform infrared spectroscopy (HATR-FTIR), low-field (1)H nuclear magnetic resonance (NMR), high-field (31)P NMR, zeta potential, differential scanning calorimetry (DSC) and UV-vis spectroscopy. The antioxidant and antitumoral activities of the genistein liposomal system were also studied. The genistein saturation concentration in ASO liposomes corresponded to 484 μM. HATR-FTIR results indicated that genistein influences the dynamics of the lipidic phosphate, choline, carbonyl and acyl chain methylenes groups. At the lipid polar head, HATR-FTIR and (31)P NMR results showed that the isoflavone reduces the hydration degree of the phosphate group, as well as its mobility. Genistein ordered the lipid interfacial carbonyl group, as evidenced by the HATR-FTIR bandwidth analysis. This ordering effect was also observed in the lipidic hydrophobic region, by HATR-FTIR, NMR, DSC and turbidity responses. At the saturation concentration, liposome-loaded genistein inhibits the lipid peroxidation induced by hydroxyl radical in 90.9%. ASO liposome-loaded genistein at 100 μM decreased C6 glioma cell viability by 57% after 72 h of treatment. Results showed an increase of the genistein in vitro activities after its incorporation in liposomes. The data described in this work will contribute to a better understanding of the interaction between genistein and a natural-source membrane and of its influence on isoflavone biological activities. Furthermore, the antitumoral results showed that genistein-based liposomes, which contain natural-sourced lipids, may be promising as a drug delivery system to be used in the glioma therapy.

Freeze-dried phosphatidylcholine liposomes encapsulating various antioxidant extracts from natural waste as functional ingredients in surimi gels

“…Slight reduction in ABTS values in PGloaded liposomes was found with respect to the corresponding phenolic extract (PG in aqueous solution). This loss of activity could be due to partial degradation of phenolic compounds during liposome preparation or to less availability of reactive groups resulting from interactions with the bilayer membrane (Lopes de Azambuja et al, 2015). In contrast, HC became apparently more antioxidant when included in liposomes, with around a 4-fold increase in ABTS value (from 20.36 to 90.40 VCEAC mg/g bioactive).…”

Dynamics of Model Membranes by NMR

“…decreases its mobility, and rigidified the hydrophobic part of the asolectine liposomes [35]. It also was found that isoflavone prevents lipid molecules from peroxidation [35].…”

“…In addition, PEG is biocompatible, which enables the possibility of further functionalization of the liposomes by attaching antibodies or ligands [33,34]. The 31 P spectra analysis of PEGylated liposomes obtained using the thin film method revealed information about the amount of free lipids or building micelles (narrow line) and lipids building MLV (broad shoulder) [31,35]. It is also possible to obtain more than one narrow signal ( Figure 2).…”

Co‐encapsulation of lupulon and xanthohumol in lecithin‐based nanoliposomes developed by sonication method

“…This is probably due to the fact that in high concentrations, the xanthohumol intends to be replaced in the core of liposome, thus, it becomes less accessible for scavenging. Slight degradation during liposome preparation and relatively low yield of encapsulation can be other explanations for this reduction (Lopes de Azambuja et al, ).…”