Physico‐chemical characterization and tissue healing changes byHancornia speciosaGomes latex biomembrane

Abstract

Skin wounds have been a public health concern of high frequency, in addition to requiring intensive and expensive care. The natural rubber latex (NRL) from Hancornia speciosa Gomes has been used to treat many problems in traditional medicine and also present healing properties, antifungal and anti‐inflammatory activity and antinociceptive effects. The purpose of this study was to characterize the new biomembrane from the NRL of H. speciosa (HS) by Fourier transform infrared (FTIR) and mechanical strength test and to investigate its biological properties by the cytotoxicity assay and in vivo healing activity. The results showed that the HS biomembrane exhibited characteristic bands of the main component cis‐1,4‐polyisoprene. Besides, its Young modulus was close to human skin with adhesive‐compatible mechanical characteristics. The cytotoxicity assays revealed that the HS biomembrane was not toxic to fibroblast cells neither using agar diffusion test nor MTT assay. Furthermore, the HS biomembrane stimulated the inflammatory cells and the angiogenesis, increased significantly the collagenesis and improved the quality of heal until the remodeling phase induced by implants in mice. Thus, this biomembrane has proven to be a safe and biocompatible biomaterial with healing potential, becoming an effective and low‐cost alternative for the treatment of skin wounds.

The collagen structure of C1q induces wound healing by engaging discoidin domain receptor 2

“…Our findings are potentially applicable in repurposing opportunities for treating inflammation and promoting wound healing. The present in vitro study would be helpful in preclinical models for wound healing studies (Caetano et al 2015 ; Pegorin et al 2021 ). Further understanding of physiological C1q binding to DDR2 is necessary for therapeutic applications in wound healing-related diseases.…”

THERAPEUTICS PERSPECTIVES OF THE Hancornia speciosa GOMES

“…The values of the elasticity modulus were close to those of human skin, in addition, cell biocompatibility and non-toxic characteristic were found, concluding that it is a safe and biocompatible biomaterial for the wound healing process due to its ability to stimulate inflammatory cells and initial angiogenesis. In addition, the study in an animal model showed an increase in collagenesis and an improvement in the quality of the healing process, suggesting that it is a good alternative to existing materials with the same function (PEGORIN et al, 2021).…”

“…Reports have also suggested a latex activity in bone regeneration, in promoting angiogenesis, as an antiinflammatory, antimicrobial and antifungal agent (PEGORIN et al, 2021), which has led to new preclinical studies, still in progress, with the use of compounds from HS latex in vivo. (Fig.…”

“…Considered a biomaterial, it has been used for the production of biomembranes, and its physical, chemical and mechanical characterization has shown a main component, cis-1,4-polyisoprene, also found in the natural latex of Hevea brasiliensis (PEGORIN et al, 2021). Previous phytochemical studies have reported the identification of different classes of compounds in HS stem latex extracts, namely chlorogenic acid, naringerin-7-O-glycoside, catechin and proanthocyanidins.…”

“…The main risk factors are old age, obesity, diabetes, lack of nutrients, chronic disease, previous leg injuries, deep vein thrombosis and phlebitis. The use of biomaterials has shown promise in the quality of life of these patients and among the available biomaterials are biocompatible natural polymers, such as natural rubber latex, cellulose, chitosan, and alginate (PEGORIN et al, 2021).…”