Physico-chemical characterization and evaluation of neat and aqueous mixtures of choline chloride + lactic acid for lignocellulosic biomass fractionation, enzymatic hydrolysis and fermentation

Kumar, Adepu K.; Shah, Ekta; Patel, Aesha; Sharma, Shubhangna; Dixit, Gaurav

doi:10.1016/j.molliq.2018.09.032

Cited by 44 publications

(17 citation statements)

References 41 publications

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“…The effect of newly synthesized DESs in pretreatment was investigated with rice straw. ChCl:LAC was regarded as a positive control since it had been applied in pretreatment of rice straw and lignin extraction [39,40]. Total sugars including glucose, xylose and arabinose were determined.…”

Section: Evaluation Of Eacl:lac In the Pretreatment Of Lignocellulosimentioning

confidence: 99%

Facilely reducing recalcitrance of lignocellulosic biomass by a newly developed ethylamine-based deep eutectic solvent for biobutanol fermentation

Xing

et al. 2020

Biotechnol Biofuels

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Background Biobutanol is promising and renewable alternative to traditional fossil fuels and could be produced by Clostridium species from lignocellulosic biomass. However, biomass is recalcitrant to be hydrolyzed into fermentable sugars attributed to the densely packed structure by layers of lignin. Development of pretreatment reagents and processes for increasing surface area, removing hemicellulose and lignin, and enhancing the relative content of cellulose is currently an area of great interest. Deep eutectic solvents (DESs), a new class of green solvents, are effective in the pretreatment of lignocellulosic biomass. However, it remains challenging to achieve high titers of total sugars and usually requires combinatorial pretreatment with other reagents. In this study, we aim to develop novel DESs with high application potential in biomass pretreatment and high biocompatibility for biobutanol fermentation. Results Several DESs with betaine chloride and ethylamine chloride (EaCl) as hydrogen bond acceptors were synthesized. Among them, EaCl:LAC with lactic acid as hydrogen bond donor displayed the best performance in the pretreatment of corncob. Only by single pretreatment with EaCl:LAC, total sugars as high as 53.5 g L−1 could be reached. Consecutive batches for pretreatment of corncob were performed using gradiently decreased cellulase by 5 FPU g−1. At the end of the sixth batch, the concentration and specific yield of total sugars were 58.8 g L−1 and 706 g kg−1 pretreated corncob, saving a total of 50% cellulase. Utilizing hydrolysate as carbon source, butanol titer of 10.4 g L−1 was achieved with butanol yield of 137 g kg−1 pretreated corncob by Clostridium saccharobutylicum DSM13864. Conclusions Ethylamine and lactic acid-based deep eutectic solvent is promising in pretreatment of corncob with high total sugar concentrations and compatible for biobutanol fermentation. This study provides an efficient pretreatment reagent for facilely reducing recalcitrance of lignocellulosic materials and a promising process for biobutanol fermentation from renewable biomass.

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Section: Evaluation Of Eacl:lac In the Pretreatment Of Lignocellulosimentioning

confidence: 99%

Facilely reducing recalcitrance of lignocellulosic biomass by a newly developed ethylamine-based deep eutectic solvent for biobutanol fermentation

Xing

et al. 2020

Biotechnol Biofuels

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show abstract

“…The effect of newly synthesized DESs in pretreatment was investigated with rice straw. ChCl:LAC was regarded as a positive control since it had been applied in pretreatment of rice straw and lignin extraction [35,36]. Total sugars including glucose, xylose and arabinose were determined.…”

Section: Evaluation Of Eacl:lac In the Pretreatment Of Lignocellulosimentioning

confidence: 99%

“…Excessive addition of Tween80 could however complicate the compositions and affect the biocompatibility of hydrolysates in biobutanol fermentation. At 0.5% Tween80, the total sugar reached 53.8 g•L − 1 , which was adequate for butanol fermentation [35]. As a result, addition of 0.5% Tween80 is selected for the hydrolysis of pretreated corncob into fermentable sugars.…”

Section: Development Of Fed-batch Pretreatment Processmentioning

confidence: 99%

Facilely Reducing Recalcitrance of Lignocellulosic Biomass by a Newly Developed Ethylamine Based Deep Eutectic Solvent for Biobutanol Fermentation

Xing

et al. 2020

Preprint

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Background: Biobutanol is promising and renewable alternative to traditional fossil fuels and could be produced by Clostridium species from lignocellulosic biomass. However, biomass is recalcitrant to be hydrolyzed into fermentable sugars attributed to the densely packed structure by layers of lignin. Development of pretreatment reagents and processes for increasing surface area, removing hemicellulose and lignin, and enhancing the relative content of cellulose is currently an area of great interest. Deep eutectic solvents (DESs), a new class of green solvents, are effective in the pretreatment of lignocellulosic biomass. However, it remains challenging to achieve high titers of total sugars and usually requires combinatorial pretreatment with other reagents. In this study, we aim to develop novel DESs with high application potential in biomass pretreatment and high biocompatibility for biobutanol fermentation.Results: Several DESs with betaine chloride and ethylamine chloride (EaCl) as hydrogen bond acceptors were synthesized. Among them, EaCl:LAC with lactic acid as hydrogen bond donor displayed the best performance in the pretreatment of corncob. Only by single pretreatment with EaCl:LAC, total sugars of as high as 53.5 g·L–1 could be reached. Consecutive batches for pretreatment of corncob were performed using gradiently decreased cellulase by 5 FPU·g–1. At the end of the sixth batch, the concentration and specific yield of total sugars were 58.8 g·L–1 and 706 g·kg–1 pretreated corncob, saving a total of 50% cellulase. Utilizing hydrolysate as carbon source, butanol titer of 10.4 g·L–1 was achieved with butanol yield of 137 g·kg–1 pretreated corncob by Clostridium saccharobutylicum DSM13864.Conclusions: Ethylamine and lactic acid based deep eutectic solvent is promising in pretreatment of corncob with high total sugar concentrations and compatible for biobutanol fermentation. This study provides an efficient pretreatment reagent for facilely reducing recalcitrance of lignocellulosic materials and a promising process for biobutanol fermentation from renewable biomass.

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“…They calculated excess molar volume (

) for those systems that found to be negative, indicating strong interactions between water and DES molecules. Additionally, volumetric properties were described for such aqueous DES systems as: allyltriphenyl phosphonium bromide (ATPPB) with diethylene glycol (DEG) [ 19 ] or triethylene glycol (TEG) [ 20 ], ChCl with ethylene glycol or glycerol [ 21 ] and ChCl with lactic acid [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Binary Mixtures of Alkanolamine Based Deep Eutectic Solvents with Water—Thermodynamic Calculation and Correlation of Crucial Physicochemical Properties

et al. 2022

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This paper demonstrates the assessment of physicochemical and thermodynamic properties of aqueous solutions of novel deep eutectic solvent (DES) built of tetrabutylammonium chloride and 3-amino-1-propanol or tetrabutylammonium bromide and 3-amino-1-propanol or 2-(methylamino)ethanol or 2-(butylamino)ethanol. Densities, speeds of sound, refractive indices, and viscosities for both pure and aqueous mixtures of DES were investigated over the entire range of compositions at atmospheric pressure and T = (293.15 ‒ 313.15) K. It was concluded that the experimental data were successfully fitted using the Jouyban–Acree model with respect to the concentration. Obtained results showed that this mathematical equation is an accurate correlation for the prediction of aqueous DES properties. Key physicochemical properties of the mixtures—such as excess molar volumes, excess isentropic compressibilities, deviations in viscosity, and deviations in refractive indices—were calculated and correlated by the Redlich–Kister equation with temperature-dependent parameters. The non-ideal behavior of the studied systems were also evaluated by using the Prigogine−Flory−Patterson theory and the results were interpreted in terms of interactions between the mixture components.

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Physico-chemical characterization and evaluation of neat and aqueous mixtures of choline chloride + lactic acid for lignocellulosic biomass fractionation, enzymatic hydrolysis and fermentation

Cited by 44 publications

References 41 publications

Facilely reducing recalcitrance of lignocellulosic biomass by a newly developed ethylamine-based deep eutectic solvent for biobutanol fermentation

Facilely reducing recalcitrance of lignocellulosic biomass by a newly developed ethylamine-based deep eutectic solvent for biobutanol fermentation

Facilely Reducing Recalcitrance of Lignocellulosic Biomass by a Newly Developed Ethylamine Based Deep Eutectic Solvent for Biobutanol Fermentation

Novel Binary Mixtures of Alkanolamine Based Deep Eutectic Solvents with Water—Thermodynamic Calculation and Correlation of Crucial Physicochemical Properties

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Physico-chemical characterization and evaluation of neat and aqueous mixtures of choline chloride + lactic acid for lignocellulosic biomass fractionation, enzymatic hydrolysis and fermentation

Cited by 44 publications

References 41 publications

Facilely reducing recalcitrance of lignocellulosic biomass by a newly developed ethylamine-based deep eutectic solvent for biobutanol fermentation

Facilely reducing recalcitrance of lignocellulosic biomass by a newly developed ethylamine-based deep eutectic solvent for biobutanol fermentation

Facilely Reducing Recalcitrance of Lignocellulosic Biomass by a Newly Developed Ethylamine Based Deep Eutectic Solvent for Biobutanol Fermentation

Novel Binary Mixtures of Alkanolamine Based Deep Eutectic Solvents with Water—Thermodynamic Calculation and Correlation of Crucial Physicochemical Properties

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Physico-chemical characterization and evaluation of neat and aqueous mixtures of choline chloride + lactic acid for lignocellulosic biomass fractionation, enzymatic hydrolysis and fermentation