Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum

Liberman, Gal Netanel; Ochbaum, Guy; Mejubovsky-Mikhelis, Margarita; Bitton, Ronit; Arad, Shoshana

doi:10.1016/j.ijbiomac.2019.09.205

Cited by 44 publications

(25 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To obtain purified polysaccharides, different membrane filtration techniques have been proposed, but this separation process poses some challenges, such as biofouling and the loss of valuable components in the permeate [63]. Additionally, the centrifugation procedure used in other studies [20] was not always successful in our case. Consequently, alternative separation methods will also be required.…”

Section: Challenges For Large-scale Production Of D Griseamentioning

confidence: 87%

“…To detect both OPA and FMOC-derivatized amino acids in one chromatogram, it was necessary to switch DAD wavelength. This switch took place between the last OPA derivative, lysine (19), and the first FMOC derivative hydroxyproline (20). For the extraction of amino acids, approximately 0.2 mg of EPS or pellet powder were mixed with 50 µL of HClO4 0.5 M, 100 µL internal standard solution, and 100 µL water.…”

Section: Quantification Of Monosaccharides and Fatty Acids By Gc-msd As Tmse Derivativesmentioning

confidence: 99%

“…The success of lubricity seemed to depend directly on the adsorption of the polymers to metal surfaces and was interlinked with the molecular weight, the molecule's structure and its functional groups. Particularly, sulfur groups are identified to mediate anti-wear protection and enhance lubricity [8,20].…”

Section: Introductionmentioning

confidence: 99%

“…The unique properties of sulfated PS initiated many research activities to find out more about their chemical composition, physicochemical properties and biosynthesis [ 28 , 30 , 31 , 32 , 33 ]. In general, sulfated PS are negatively charged, and the prominent monosaccharides were found to be xylose, glucose and galactose, which fall into different ratios, depending on growth conditions [ 20 , 34 , 35 , 36 ]. In addition to other sugars in smaller proportions and the sulfate content (1–9% w / w ) already mentioned, proteins covalently and non-covalently bound to the PS represent an important component [ 37 , 38 , 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

“…As previously mentioned, data on the molecular composition of sulfated PS in D. grisea and also its production under different conditions are available [ 20 , 35 , 37 ]. However, we aimed for a holistic approach by evaluating the complete culture of D. grisea , including its environmental impact, and therefore addressing the need of a biorefinery approach in algae biotechnology.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Potential of the Red Alga Dixoniella grisea for the Production of Additives for Lubricants

Gavalás-Olea

Siol

Sakka

et al. 2021

Plants

View full text Add to dashboard Cite

There is an increasing interest in algae-based raw materials for medical, cosmetic or nutraceutical applications. Additionally, the high diversity of physicochemical properties of the different algal metabolites proposes these substances from microalgae as possible additives in the chemical industry. Among the wide range of natural products from red microalgae, research has mainly focused on extracellular polymers for additive use, while this study also considers the cellular components. The aim of the present study is to analytically characterize the extra- and intracellular molecular composition from the red microalga Dixoniella grisea and to evaluate its potential for being used in the tribological industry. D. grisea samples, fractionated into extracellular polymers (EPS), cells and medium, were examined for their molecular composition. This alga produces a highly viscous polymer, mainly composed of polysaccharides and proteins, being secreted into the culture medium. The EPS and biomass significantly differed in their molecular composition, indicating that they might be used for different bio-additive products. We also show that polysaccharides and proteins were the major chemical compounds in EPS, whereas the content of lipids depended on the separation protocol and the resulting product. Still, they did not represent a major group and were thus classified as a potential valuable side-product. Lyophilized algal fractions obtained from D. grisea were found to be not toxic when EPS were not included. Upon implementation of EPS as a commercial product, further assessment on the environmental toxicity to enchytraeids and other soil organisms is required. Our results provide a possible direction for developing a process to gain an environmentally friendly bio-additive for application in the tribological industry based on a biorefinery approach.

show abstract

Section: Challenges For Large-scale Production Of D Griseamentioning

confidence: 87%

Section: Quantification Of Monosaccharides and Fatty Acids By Gc-msd As Tmse Derivativesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%