Physico‐Chemical and Biochemical Properties of Bioconjugates

Ahmed, Marya; Narain, Ravin

doi:10.1002/9781118775882.ch18

Cited by 1 publication

(2 citation statements)

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“…Capping agents—enzyme conjugates—are essential considerations in biosensor preparations. In many cases, biocompatible capping agents are commonly used [ 1 , 2 , 22 , 23 , 24 ]. Capping agents may improve enzyme stability, shield enzymes from undesired interactions, and prolong shelf-life [ 23 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…In many cases, biocompatible capping agents are commonly used [ 1 , 2 , 22 , 23 , 24 ]. Capping agents may improve enzyme stability, shield enzymes from undesired interactions, and prolong shelf-life [ 23 , 25 , 26 ]. Such shielding effects rely on the physicochemical properties of the capping agents, such as the hydrophilicity, chain length, and architecture [ 25 , 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Capping Agents on Gold Nanostar Stability, Functionalization, and Colorimetric Biosensing Capability

2022

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Capping agents (organic ligands, polymers, and surfactants) are pivotal for stabilizing nanoparticles; however, they may influence the surface chemistry, as well as the physico-chemical and biological characteristics, of gold nanostar (AuNS)-based biosensors. In this study, we proved that various capping agents affected capped and bioconjugated AuNS stability, functionality, biocatalysis, and colorimetric readouts. Capped and bioconjugated AuNSs were applied as localized surface plasmon resonance (LSPR)-based H2O2 sensors using glucose oxidase (GOx) as a model enzyme. Furthermore, our analyses revealed that the choice of capping agent influenced the properties of the AuNSs, their stability, and their downstream applications. Our analyses provide new insights into factors governing the choice of capping agents for gold nanostars and their influences on downstream applications with conjugated enzymes in confined environments.

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