Physical study of film-forming acrylate emulsion polymers for biosensor applications

Gooding, J. Justin; Hall, Carl E.; Hall, Elizabeth A. H.

doi:10.1016/s0003-2670(97)00282-1

Cited by 18 publications

(13 citation statements)

References 27 publications

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“…Situmorang et al have reported some creative and pioneering work about electrodeposition of polymers for amperometric biosensor development. [5][6][7] The enzymatic biosensors based on the use of non-conducting polymer films have faster response time and better antiinterferent ability due to the films being thinner and more compact films than those based on the use of conducting polymer films. 4,8 o-Aminophenol (o-AP) can be electropolymerized to form an electro-inactive film when the pH of the solution is over 3.…”

Section: Introductionmentioning

confidence: 99%

An Amperometric Glucose Biosensor Based on Glucose Oxidase Immobilized in Electropolymerized Poly(o-aminophenol) and Carbon Nanotubes Composite Film on a Gold Electrode

et al. 2005

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An amperometric glucose biosensor is developed that is based on immobilization of glucose oxidase (GOD) in a composite film of poly(o-aminophenol) (POAP) and carbon nanotubes (CNT), which are electrochemically copolymerized at a gold (Au) electrode. Because of the high surface per volume ratio and excellent electrical conductivity of CNT, the biosensor based on an Au/POAP/CNT/GOD electrode has lower detection limit (0.01 mM), larger maximum response current (0.24 mA cm -2 ) and higher sensitivity (11.4 mA M -1 cm -2 ) than the values of the biosensor based on an Au/POAP/GOD electrode. Additionally, the biosensor shows fast response time, large response current, and good antiinterferent ability for ascorbic acid, uric acid and acetaminophen. Good reproducibility and stability of the biosensor are also observed.

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Section: Introductionmentioning

confidence: 99%

An Amperometric Glucose Biosensor Based on Glucose Oxidase Immobilized in Electropolymerized Poly(o-aminophenol) and Carbon Nanotubes Composite Film on a Gold Electrode

et al. 2005

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“…However, in this preliminary article the enzyme was immobilized within a hydrophilic emulsion polymer [23] which was deposited in a well upstream of the detector electrode. This method gave a poorly defined enzyme layer where it was ambiguous whether the hydrogen peroxide produced in the enzyme reaction was derived from enzyme confined to the surface of the enzyme layer or from throughout the entire polymer membrane.…”

Section: Introductionmentioning

confidence: 99%

Which Parameters Affect the Response of the Channel Biosensor?

et al. 2003

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The important parameters in defining the response of the portable channel biosensor described previously are explored by connecting the portable flow cell to a gravity feed flow system and using a highly defined enzyme immobilization protocol which ensures the enzyme reaction is a surface reaction. The enzyme glucose oxidase (GOD) was immobilized by covalent attachment to a self-assembled monolayer modified gold surface. As a glucose solution flowed down the rectangular duct defined by the flow cell, it passed over the enzyme layer where the enzyme reaction produced hydrogen peroxide. The hydrogen peroxide was swept further downstream to the detector electrode. The response of such an enzyme electrode was shown to be limited by mass transport of the cosubstrate oxygen to the enzyme layer. Increasing the amount of oxygen in the sample meant the response of the biosensor became limited by the enzyme kinetics. The influence of parameters such as flow rate, height of the channel, enzyme layer length and the gap between the enzyme layer and the detector electrode were explored.

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“…In this process the enzyme was immobilized by entrapment in the polymer. To prevent leaching of the enzyme out of the latex film [4], the entrapped glucose oxidase was covalently attached to the carboxyl group of the polymer backbone using 0.015 M EDC and 0.03 M NHS in phosphate buffer solution (pH 5.5) for one hour. The EDC and NHS converted the carboxylic acid moieties of the acrylic acids of the polymer into a succinimide ester susceptible to nucleophilic attack from amines [14].…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, acrylate based latex polymers have been shown to have good film forming properties [4,12] which are inert to the enzyme reaction [4]. Latex polymers synthesized using either 5-hydroxymethyl-bicyclo[2.2.1.…”

Section: Introductionmentioning

confidence: 99%

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Using the Aggregation of Latex Polymers in the Fabrication of Reproducible Enzyme Electrodes

et al. 2003

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An enzyme electrode for glucose is described as a model system to demonstrate a fabrication method using latex aggregation and entrapment of enzyme. Electrosterically-stabilized latex particles synthesized by emulsion polymerization in batch from acrylic acid, methyl methacrylate and butyl acrylate, and glucose oxidase were coagulated together at pH 5.5 with ethanol. A platinum disk electrode dipped in the solution becomes coated with latex/enzyme. The relative thickness of the film and relative amount of enzyme may be controlled by the time the electrode is in contact with the solution. The enzyme was then immobilized by covalent attachment of amine groups to carboxylic moieties in the polymer using 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide hydrochloride and N-hydroxysuccinimide. Five minutes contact with the latex/enzyme solution and subsequent amide coupling, gave electrodes with a reproducibility of 5.7% RSD, a wide dynamic range (0 ± 100 mM) and good storage properties.

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Physical study of film-forming acrylate emulsion polymers for biosensor applications

Cited by 18 publications

References 27 publications

An Amperometric Glucose Biosensor Based on Glucose Oxidase Immobilized in Electropolymerized Poly(o-aminophenol) and Carbon Nanotubes Composite Film on a Gold Electrode

An Amperometric Glucose Biosensor Based on Glucose Oxidase Immobilized in Electropolymerized Poly(o-aminophenol) and Carbon Nanotubes Composite Film on a Gold Electrode

Which Parameters Affect the Response of the Channel Biosensor?

Using the Aggregation of Latex Polymers in the Fabrication of Reproducible Enzyme Electrodes

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