Physical stress as a model to study variations in ADAMTS‐13 activity, von Willebrand factor level and platelet activation

Claus, Ralf A.; Bockmeyer, Clemens L.; Soßdorf, Maik; Lösche, Wolfgang; Hilberg, Thomas

doi:10.1111/j.1538-7836.2006.01837.x

Cited by 17 publications

(20 citation statements)

References 30 publications

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“…In fact, previous reports indicate their increase after exercise both in rat aortas 59 and in human skeletal muscles. 60 In agreement with previous reports, our results show that the von Willebrand Factor (vWF), 61,62 ADAMTS13 (ref. 61) and P-selectin (SELP) 62,63 are all significantly upregulated after exercise.…”

Section: Effect Of Exercise On the Plasma Proteomesupporting

confidence: 93%

“…60 In agreement with previous reports, our results show that the von Willebrand Factor (vWF), 61,62 ADAMTS13 (ref. 61) and P-selectin (SELP) 62,63 are all significantly upregulated after exercise. It was previously shown that the state of physical fitness of the subjects strongly influences the magnitude of the increase in vWF after additional exercise.…”

Section: Effect Of Exercise On the Plasma Proteomesupporting

confidence: 93%

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Effect of exercise on the plasma vesicular proteome: a methodological study comparing acoustic trapping and centrifugation

et al. 2018

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a Extracellular vesicles (EVs) are a heterogeneous group of actively released vesicles originating from a wide range of cell types. Characterization of these EVs and their proteomes in the human plasma provides a novel approach in clinical diagnostics, as they reflect physiological and pathological states. However, EV isolation is technically challenging with the current methods having several disadvantages, requiring large sample volumes, and resulting in loss of sample and EV integrity. Here, we use an alternative, non-contact method based on a microscale acoustic standing wave technology. Improved coupling of the acoustic resonator increased the EV recovery from 30% in earlier reports to 80%, also displaying long term stability between experiment days. We report a pilot study, with 20 subjects who underwent physical exercise. Plasma samples were obtained before and 1 h after the workout. Acoustic trapping was compared to a standard high-speed centrifugation protocol, and the method was validated by flow cytometry (FCM). To monitor the device stability, the pooled frozen plasma from volunteers was used as an internal control. A key finding from the FCM analysis was a decrease in CD62E+ (E-selectin) EVs 1 h after exercise that was consistent for both methods. Furthermore, we report the first data that analyse differential EV protein expression before and after physical exercise. Olink-based proteomic analysis showed 54 significantly changed proteins in the EV fraction in response to physical exercise, whereas the EV-free plasma proteome only displayed four differentially regulated proteins, thus underlining an important role of these vesicles in cellular communication, and their potential as plasma derived biomarkers. We conclude that acoustic trapping offers a fast and efficient method comparable with high-speed centrifugation protocols. Further, it has the advantage of using smaller sample volumes (12.5 μL) and rapid contact-free separation with higher yield, and can thus pave the way for future clinical EV-based diagnostics.

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Section: Effect Of Exercise On the Plasma Proteomesupporting

confidence: 93%

Section: Effect Of Exercise On the Plasma Proteomesupporting

confidence: 93%

Effect of exercise on the plasma vesicular proteome: a methodological study comparing acoustic trapping and centrifugation

et al. 2018

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“…A previous study showed similar results as ADAMTS13 increased after exercise in patients with von Willebrand disease type 2B [3]. However, in another study in healthy individuals there was no difference in ADAMTS13 between baseline and immediately after exercise [3], [5]. In addition, ADAMTS13 decreased after infusion of desmopressin, which induces a release of large VWF multimers into the circulation, in healthy individuals [32].…”

Section: Discussionmentioning

confidence: 53%

Performance Related Factors Are the Main Determinants of the von Willebrand Factor Response to Exhaustive Physical Exercise

et al. 2014

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BackgroundPhysical stress triggers the endothelium to release von Willebrand Factor (VWF) from the Weibel Palade bodies. Since VWF is a risk factor for arterial thrombosis, it is of great interest to discover determinants of VWF response to physical stress. We aimed to determine the main mediators of the VWF increase by exhaustive physical exercise.Methods105 healthy individuals (18–35 years) were included in this study. Each participant performed an incremental exhaustive exercise test on a cycle ergometer. Respiratory gas exchange measurements were obtained while cardiac function was continuously monitored. Blood was collected at baseline and directly after exhaustion. VWF antigen (VWF:Ag) levels, VWF collagen binding (VWF:CB) levels, ADAMTS13 activity and common variations in Syntaxin Binding Protein-5 (STXBP5, rs1039084 and rs9399599), Syntaxin-2 (STX2, rs7978987) and VWF (promoter, rs7965413) were determined.ResultsThe median VWF:Ag level at baseline was 0.94 IU/mL [IQR 0.8–1.1] and increased with 47% [IQR 25–73] after exhaustive exercise to a median maximum VWF:Ag of 1.38 IU/mL [IQR 1.1–1.8] (p<0.0001). VWF:CB levels and ADAMTS13 activity both also increased after exhaustive exercise (median increase 43% and 12%, both p<0.0001). The strongest determinants of the VWF:Ag level increase are performance related (p<0.0001). We observed a gender difference in VWF:Ag response to exercise (females 1.2 IU/mL; males 1.7 IU/mL, p = 0.001), which was associated by a difference in performance. Genetic variations in STXBP5, STX2 and the VWF promoter were not associated with VWF:Ag levels at baseline nor with the VWF:Ag increase.ConclusionsVWF:Ag levels strongly increase upon exhaustive exercise and this increase is strongly determined by physical fitness level and the intensity of the exercise, while there is no clear effect of genetic variation in STXBP5, STX2 and the VWF promoter.

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“…ADAMTS13 activity was determined using a von Willebrand factor fluorescent resonance energy transfer 73 (VWF-FRETS73) substrate as previously described (43,44). von Willebrand factor antigen (VWF:Ag), von Willebrand collagen binding activity (VWF:CB), von Willebrand ristocetin cofactor assay (VWF:RCo), VWF propeptides were determined as described previously (9,30,45).…”

Section: Characterization Of Septic Patients and Determination Of Labmentioning

confidence: 99%

Preserved Expression of mRNA Coding von Willebrand Factor-Cleaving Protease ADAMTS13 by Selenite and Activated Protein C

Ekaney

Bockmeyer

Soßdorf

et al. 2015

Mol Med

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Physical stress as a model to study variations in ADAMTS‐13 activity, von Willebrand factor level and platelet activation

Cited by 17 publications

References 30 publications

Effect of exercise on the plasma vesicular proteome: a methodological study comparing acoustic trapping and centrifugation

Effect of exercise on the plasma vesicular proteome: a methodological study comparing acoustic trapping and centrifugation

Performance Related Factors Are the Main Determinants of the von Willebrand Factor Response to Exhaustive Physical Exercise

Preserved Expression of mRNA Coding von Willebrand Factor-Cleaving Protease ADAMTS13 by Selenite and Activated Protein C

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