Physical Shock Wave Mediates Membrane Hyperpolarization and Ras Activation for Osteogenesis in Human Bone Marrow Stromal Cells

Wang, Feng‐Sheng; Wang, Ching‐Jen; Huang, Hui‐Shan; Chung, Hou; Chen, Rong‐Fu; Yang, Kuender D.

doi:10.1006/bbrc.2001.5654

Cited by 163 publications

(155 citation statements)

References 36 publications

Supporting

Mentioning

129

Contrasting

Unclassified

Order By: Relevance

“…Osteoblasts were washed and resuspended with complete medium. Cell suspensions were subjected to ESW treatment with modification as described before (27,28). Cells were suspended in 15-ml sterile polystyrene tubes at a concentration of 1 ϫ 10 6 /ml.…”

Section: Methodsmentioning

confidence: 99%

Optimal Intensity Shock Wave Promotes the Adhesion and Migration of Rat Osteoblasts via Integrin β1-mediated Expression of Phosphorylated Focal Adhesion Kinase

Chen

et al. 2012

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background:The effects of extracorporeal shock wave (ESW) on adhesion and migration of osteoblasts have not been reported until now. Results: Optimal intensity shock wave promotes osteoblast adhesion and migration. Conclusion: ESW promotes the adhesion and migration of osteoblasts via integrin ␤1-mediated expression of phosphorylated FAK.Significance: This provides a mechanistic basis for improving the effectiveness of ESW treatment in fracture healing and tissue engineering.

show abstract

Section: Methodsmentioning

confidence: 99%

Optimal Intensity Shock Wave Promotes the Adhesion and Migration of Rat Osteoblasts via Integrin β1-mediated Expression of Phosphorylated Focal Adhesion Kinase

Chen

et al. 2012

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Because the physical forces generated by cavitation are highly localized, SW could induce localized stress and subsequent shear stress on cell surface membranes (Fisher et al 2001). Several biochemical effects of SW were reported including hyperpolarization, Ras activation, non-enzymatic NO synthesis, and induction of stress fibers and intercellular gaps (Seidl et al 1994;Wang et al 2001;Gotte et al 2002). Also, SW treatment is reported to affect the expression of several chemokines and matrix metalloproteinases and therefore bring anti-inflamatory effects Mariotto et al 2005;Stojadinovic et al 2008;Mariotto et al 2009) in addition to up-regulation of VEGF and its receptor Flt-1 (Nishida et al 2004), implying that the multiple angiogenic pathways are involved in the beneficial effects of the SW therapy.…”

Section: Mechanisms For Sw-induced Neovascularizationmentioning

confidence: 99%

Extracorporeal Shock Wave Therapy as a New and Non-invasive Angiogenic Strategy

Ito

Shimokawa

2009

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

“…Color images available online at www.liebertpub.com/tea hFOB osteogenesis on 3D printed nucleated TPU Osteogenesis of hFOBs is an important phase in de novo osseous tissue formation. Several forms of stem cell and mature osteoblast osteoinduction have been employed in an effort to stimulate enhanced tissue formation including mechanical [26][27][28] and chemical stimulation. [29][30][31] Three-week osteogenesis of hFOBs on nucleated 3D printed TPU scaffolds was conducted in this study.…”

Section: Synthesis and Characterization Of Sbf Nucleated 3d Printed Tmentioning

confidence: 99%

Simulated Body Fluid Nucleation of Three-Dimensional Printed Elastomeric Scaffolds for Enhanced Osteogenesis

Castro

Tan

Shen

et al. 2016

Tissue Engineering Part A

View full text Add to dashboard Cite

Osseous tissue defects caused by trauma present a common clinical problem. Although traditional clinical procedures have been successfully employed, several limitations persist with regards to insufficient donor tissue, disease transmission, and inadequate host-implant integration. Therefore, this work aims to address current limitations regarding inadequate host tissue integration through the use of a novel elastomeric material for three-dimensional (3D) printing biomimetic and bioactive scaffolds. A novel thermoplastic polyurethanebased elastomeric composite filament (Gel-Lay) was used to manufacture porous scaffolds. In an effort to render the scaffolds more bioactive, the flexible scaffolds were subsequently incubated in simulated body fluid at various time points and evaluated for enhanced mechanical properties along with the effects on cell adhesion, proliferation, and 3-week osteogenesis. This work is the first reported use of a novel class of flexible elastomeric materials for the manufacture of 3D printed bioactive scaffold fabrication allowing efficient and effective nucleation of hydroxyapatite (HA) leading to increased nanoscale surface roughness while retaining the bulk geometry of the predesigned structure. Scaffolds with interconnected microfibrous filaments of *260 mm were created and nucleated in simulated body fluid that facilitated cell adhesion and spreading after only 24 h in culture. The porous structure further allowed efficient nucleation, exchange of nutrients, and metabolic waste removal during new tissue formation. Through the incorporation of osteoconductive HA, human fetal osteoblast adhesion and differentiation were greatly enhanced thus setting the tone for further exploration of this novel material for biomedical and tissue regenerative applications.

show abstract

Physical Shock Wave Mediates Membrane Hyperpolarization and Ras Activation for Osteogenesis in Human Bone Marrow Stromal Cells

Cited by 163 publications

References 36 publications

Optimal Intensity Shock Wave Promotes the Adhesion and Migration of Rat Osteoblasts via Integrin β1-mediated Expression of Phosphorylated Focal Adhesion Kinase

Optimal Intensity Shock Wave Promotes the Adhesion and Migration of Rat Osteoblasts via Integrin β1-mediated Expression of Phosphorylated Focal Adhesion Kinase

Extracorporeal Shock Wave Therapy as a New and Non-invasive Angiogenic Strategy

Simulated Body Fluid Nucleation of Three-Dimensional Printed Elastomeric Scaffolds for Enhanced Osteogenesis

Contact Info

Product

Resources

About