Physical properties of a single sugar α-linked glucuronic acid-based oligosaccharide produced by a Gluconacetobacter hansenii strain

Khan, Taous; Khan, Hamayun; Park, Joong Kon

doi:10.1016/j.procbio.2006.08.006

Cited by 15 publications

(21 citation statements)

References 30 publications

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“…Gluconacetobacter hansenii PJK is known to produce glucuronic acid oligomers [4,5] which, due to the structures similarities with alginate oligomers, are expected to have at least eliciting activities [6,7]. Moreover G. hansenii was formerly named Acetobacter hansenii by Gosselé [8] and the genus Acetobacter contains species which produce other water-soluble heteropolysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Production and Preliminary Characterization of New Exopolysaccharides from <i>Gluconacetobacter hansenii</i> LMG1524

Valepyn¹,

Berezina²,

Paquot³

2012

AiM

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The influence of different carbon and nitrogen sources, of ethanol concentration, the optimal pH, temperature and medium composition were evaluated on extracellular polysaccharides (EPS) synthesis and bacterial growth of Gluconacetobacter hansenii LMG1524, and preliminary characterization of EPS was investigated. The highest EPS yields were obtained using glycerol and ammonium sulphate as carbon and nitrogen sources, respectively. The increase of ethanol concentration in the medium did not influence the EPS synthesis but reduced the bacterial growth. The optimum temperature and pH for polysaccharides production were respectively 25˚C and 5; whereas for cell growth were respectively 30˚C and 4. The optimal culture medium composition was determined as follows: 10 g/L sucrose, 0.892 g/L (NH 4 ) 2 SO 4 , 0.34 g/L NaNO 3 , 3 mL acetic acid, 1.5 g/L KH 2 PO 4 , 1.5 g/L K 2 HPO 4 and 0.6 g/L MgSO 4 . The polysaccharides produced were of 14 and 10 polymerization degrees (DP) and constituted mainly of glucose, galactose and mannose, in relative percent of 36.36, 33.94 and 22.42, respectively.

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Section: Introductionmentioning

confidence: 99%

Optimization of Production and Preliminary Characterization of New Exopolysaccharides from <i>Gluconacetobacter hansenii</i> LMG1524

Valepyn¹,

Berezina²,

Paquot³

2012

AiM

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“…The polysaccharide structure was also investigated using NMR spectroscopy. The lack of signals around the 2 ppm region confirmed the absence of any O-acetyl groups (Khan et al, 2007;Park et al, 2006). The peak for the anomeric proton appeared as a doublet with a small coupling constant which indicated clearly the ␣-configuration (Khan et al, 2007;Park et al, 2006).…”

Section: 2˛-(14)-d-polyglucuronic Acid From Gluconacetobacter Hanmentioning

confidence: 84%

“…The strain was grown on a basal medium containing glucose (10 g/L), yeast extract (10 g/L), peptone (7 g/L), acetic acid (0.15%, v/v), and succinate (0.2 g/L). The water-soluble polysaccharides produced were isolated from the supernatant of culture broths by ethanolic precipitation followed by centrifugation (Khan, Hyun, & Park, 2007;Park, Khan, & Jung, 2006). The dried watersoluble polysaccharides were subjected to acid hydrolysis and the hydrolysates were analyzed using HPLC.…”

Section: 2˛-(14)-d-polyglucuronic Acid From Gluconacetobacter Hanmentioning

confidence: 99%

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Polyglucuronic acids: Structures, functions and degrading enzymes

Elboutachfaiti

Delattre

Petit

et al. 2011

Carbohydrate Polymers

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“…The major dissimilarities included the presence of O-acetyl and O-methyl groups and a lack of unsaturation in the terminal unit of the non-reducing end of WSOS [17]. WSOS showed a thermal stability better or comparable to the other microbial polysaccharides of industrial potential and may be useful as auxiliary emulsifying agent [18]. In the present experiments, the production of this valuable by-product was also monitored.…”

Section: Production Of Wsos In Batch and Fed-batch Cultivationmentioning

confidence: 93%

Production of bacterial cellulose in static conditions by a simple fed-batch cultivation strategy

Shezad

Khan

et al. 2009

Korean J. Chem. Eng.

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The current study investigated fed-batch cultivation for the increased productivity of bacterial cellulose (BC) sheets by Gluconacetobacter hansenii PJK in static conditions using chemically defined medium and waste from beer fermentation broth. Fermentations were carried out in a 3 L jar fermenter without any impeller for 30 days. In the proposed fed-batch cultivation, 500 mL of the medium was initially inoculated with pre-culture in a jar fermenter while a fresh medium was fed periodically. BC production was also done by using batch cultivation which was used as a control for comparison. The results obtained revealed an overall of 2-3 times increase in BC production in fedbatch cultivation compared to batch cultivation after 30 days of cultivation. During these experiments, it was found that waste from beer fermentation broth is a superior medium for the BC production using fed-batch cultivation. The production of water soluble oligosaccharides as useful by-products was also monitored during these investigations. Fed-batch cultivation and waste from beer fermentation was also found superior for the production of these by-products. According to literature search and to the best of our knowledge, it is the first report of using fed-batch cultivation for BC production in static conditions.

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Physical properties of a single sugar α-linked glucuronic acid-based oligosaccharide produced by a Gluconacetobacter hansenii strain

Cited by 15 publications

References 30 publications

Optimization of Production and Preliminary Characterization of New Exopolysaccharides from <i>Gluconacetobacter hansenii</i> LMG1524

Optimization of Production and Preliminary Characterization of New Exopolysaccharides from <i>Gluconacetobacter hansenii</i> LMG1524

Polyglucuronic acids: Structures, functions and degrading enzymes

Production of bacterial cellulose in static conditions by a simple fed-batch cultivation strategy

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Physical properties of a single sugar α-linked glucuronic acid-based oligosaccharide produced by a Gluconacetobacter hansenii strain

Cited by 15 publications

References 30 publications

Optimization of Production and Preliminary Characterization of New Exopolysaccharides from &lt;i&gt;Gluconacetobacter hansenii&lt;/i&gt; LMG1524

Optimization of Production and Preliminary Characterization of New Exopolysaccharides from &lt;i&gt;Gluconacetobacter hansenii&lt;/i&gt; LMG1524

Polyglucuronic acids: Structures, functions and degrading enzymes

Production of bacterial cellulose in static conditions by a simple fed-batch cultivation strategy

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Optimization of Production and Preliminary Characterization of New Exopolysaccharides from <i>Gluconacetobacter hansenii</i> LMG1524

Optimization of Production and Preliminary Characterization of New Exopolysaccharides from <i>Gluconacetobacter hansenii</i> LMG1524