Permafrost-affected soils contain about 1,000 Pg of soil organic carbon (SOC) in the uppermost 3 m (Mishra et al., 2021). The Arctic is experiencing one of the greatest impacts of climate change in the world (IPCC, 2022). Record high permafrost temperatures were registered in the last two decades (Biskaborn et al., 2019), leading to permafrost thaw. The microbial decomposition of thawing permafrost organic matter (OM) releases the greenhouse gases (GHG) carbon dioxide (CO 2 ) and methane (CH 4 ) (Lindroth et al., 2022;Miner et al., 2022;Schuur et al., 2015). Methane has at least a 28-fold global warming potential of CO 2 (Myhre et al., 2013). Hence, we need to understand the relative emission of CO 2 to CH 4 when permafrost-affected soils warm and permafrost thaw.The formation of CO 2 and CH 4 from thawing permafrost has been studied most often by laboratory incubations. Using this method, a wide range (<1 to >1,000) of ratios between CO 2 and CH 4 production in permafrost-affected soils have been reported (