Physical phase compensation in digital holographic microscopy

Qu, Weijuan; Chee, Oi Choo; Yu, Yingjie; Singh, Vijay Raj; Asundi, Anand

doi:10.1117/12.851399

Cited by 3 publications

(3 citation statements)

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“…In order to compensate the quadratic phase aberration, the telecentric arrangement demanded precise adjustment of the distance between the MO and the collimated lens. The adjustment was realized by way of observation, but the analyses found that observing the interference fringes' shape [28] or the Fourier spectrum's distribution of hologram [25][26][27][28] may fail, and results in the residual phase curvature [25,73]. Aiming at the situation, a posteriori numerical compensation based on the Zernike surface fitting was performed.…”

Section: Appl Sci 2018 8 X For Peer Review 9 Of 20mentioning

confidence: 99%

“…In order to suppress the zero-order and the twin-image, the phase-shifting method and frequency filter method provide a notable solution, avoiding the overlapping of the reconstructed image [1,[15][16][17][18][19][20][21]. Due to a phase distortion introduced by the MO, physical or numerical compensation methods may be adopted to eliminate the quadratic phase distortion and other phase distortions [22][23][24][25][26][27][28][29][30]. Because the coherent source produces the inherent coherent noise in the reconstructed image, the low-coherent resource, multiplexing holograms methods, and digital processing methods have been studied to improve the imaging quality, respectively [31][32][33][34][35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

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Recent Progress on Aberration Compensation and Coherent Noise Suppression in Digital Holography

Liu

Wang

2018

Applied Sciences

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Digital holographic microscopy (DHM) is a topographic measurement technique that permits full-field, nondestructive, dynamic, quantitative amplitude, and phase-contrast imaging. The technique may realize the lateral resolution with submicron scale and the longitudinal resolution with subnanometer scale, respectively. Improving imaging quality has always been the research focus in DHM since it has a direct effect on the precise topographic measurement. In this paper, the recent progress on phase aberration compensation and coherent noise suppression is reviewed. Included in this review are the hologram spectrum's centering judgment methods of side band in tilt phase error compensation, the physical and numerical compensation methods in phase aberration compensation, and the single-shot digital process methods in coherent noise suppression. The summaries and analyses for these approaches can contribute to improving the imaging quality and reducing the measurement error of DHM, which will further promote the wider applications of DHM in the topographic measurement fields, such as biology and micro-electro mechanical systems.

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Section: Appl Sci 2018 8 X For Peer Review 9 Of 20mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Recent Progress on Aberration Compensation and Coherent Noise Suppression in Digital Holography

Liu

Wang

2018

Applied Sciences

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“…This is because, aiming to save space, commercial microscopes are usually mounted in a nontelecentric configuration. In such cases, it is still possible to cancel out the parabolic phase, provided that the sample is illuminated with a specific spherical wavefront [15,20,21]. The problem of this solution is that the illumination architecture is fully adapted to the magnification of the microscope objective (MO).…”

Section: Introductionmentioning

confidence: 99%

Physical compensation of phase curvature in digital holographic microscopy by use of programmable liquid lens

et al. 2015

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Quantitative phase measurements obtained with digital holographic microscopes are strongly dependent on the optical arrangement of the imaging system. The nontelecentric operation provides phase measurements affected by a parabolic phase factor and requires numerical postprocessing, which does not always remove all the perturbation. Accurate phase measurements are achieved by using the imaging system in telecentric mode. Unfortunately, this condition is not accomplished when a commercial microscope is used as the imaging system. In this paper, we present an approach for obtaining accurate phase measurements in nontelecentric imaging systems without the need for numerical postprocessing. The method uses an electrically tunable liquid lens to illuminate the sample so that the perturbing parabolic wavefront is cancelled out. Experimental holograms of a Fresnel lens and a section of the thorax of a Drosophila melanogaster fly are captured to verify the proposed method.

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Quantitative and Dynamic Phase Imaging of Biological Cells by the Use of the Digital Holographic Microscopy Based on a Beam Displacer Unit

Wang

et al. 2018

IEEE Photonics J.

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The digital holographic microscopy can be used for the quantitative and dynamic phase imaging of biological cells, which has a very wide application in the fields of biological and medical sciences. To make the measurement system simple, compact, and low-cost, a common-path configuration based on a beam displacer unit is introduced in the digital holographic microscopy. The simple optical structure and common-path design reduces the system requirement for the light source coherence, realizes the convenient adjustment of the object and reference beams and achieves an excellent system temporal stability of 0.53 nm. The living mouse osteoblastic cells during mitotic phase are quantitatively measured to demonstrate the capability and applicability of the system.

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Physical phase compensation in digital holographic microscopy

Cited by 3 publications

References 0 publications

Recent Progress on Aberration Compensation and Coherent Noise Suppression in Digital Holography

Recent Progress on Aberration Compensation and Coherent Noise Suppression in Digital Holography

Physical compensation of phase curvature in digital holographic microscopy by use of programmable liquid lens

Quantitative and Dynamic Phase Imaging of Biological Cells by the Use of the Digital Holographic Microscopy Based on a Beam Displacer Unit

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