Physical Methods of Microalgal Biomass Pretreatment

Abstract

The prospect of depletion of natural energy resources on the Earth forces researchers to seek and explore new and alternative energy sources. Biomass is a composite resource that can be used in many ways leading to diversity of products. Therefore, microalgal biomass offers great potential. The main aim of this study is to find the best physical method of microalgal biomass pretreatment that guarantees efficient lipid extraction. These studies identifies biochemical composition of microalgal biomass as source for biodisel production. The influence of drying at different temperatures and lyophilization was investigated. In addition, wet and untreated biomass was examined. Cell disruption (sonication and microwave) techniques were used to improve lipid extraction from wet biomass. Additionally, two different extraction methods were carried out to select the best method of crude oil extraction. The results of this study show that wet biomass after sonication is the most suitable for extraction. The fatty acid composition of microalgal biomass includes linoleic acid (C18:2), palmitic acid (C16:0), oleic acid (C18:1), linolenic acid (C18:3), and stearic acid (C18:0), which play a key role in biodiesel production.

Cultivation of Chlorella protothecoides in photobioreactors: The combined impact of photoperiod and CO2 concentration

“…Cheng et al [32] reported that the maximum biomass concentration of C. protothecoides after 11-day cultivation in proteose medium was about 0.8 g L −1 under conditions of 12/12 on/off light cycle and 200 μmol/m 2 radiation. Also, Piasecka and Krzemińska [33] reported in their investigation that C. protothecoides was shown to produce 0.95 g L −1 on Polytoma medium in photoheterotrophic growth conditions under the 16/8 light/dark cycle.…”

Stimulatory effect of indole-3-acetic acid and continuous illumination on the growth of Parachlorella kessleri

“…The biomass doubling time T d (h) of P. kessleri microalgae was determined based on the specific growth rate (μ) using the following formula (Piasecka et al, 2014):…”

Optimization of Ultrasound Assisted Extraction of Functional Ingredients from Stevia Rebaudiana Bertoni Leaves

“…In recent times, significant development of modern extraction techniques such as ultrasound assisted extraction (UAE), microwave assisted extraction, enzyme assisted extraction, and supercritical fluid extraction have emerged (Barba et al, 2015;Khoddami, et al, 2013;Krishnaswamy et al, 2013;Miron et al, 2013, Piasecka et al, 2014Puri et al, 2012). Ultrasound assisted extraction has numerous advantages, primarily because of the equipment flexibility, easy handling, and cost effectiveness in combination with a shorter treatment time, reduction of temperatures, high reproducibility, improvement in mass transfer, and the eco-friendly approach (Barba et al, 2014b;Brnčić et al, 2009).…”