PHYSICAL METHODS FOR OBTAINING SYNCHRONOUS CULTURE OF
            <i>ESCHERICHIA COLI</i>

Maruyama, Y; Yanagita, Tomomichi

doi:10.1128/jb.71.5.542-546.1956

Cited by 71 publications

(18 citation statements)

References 10 publications

(8 reference statements)

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“…Several workers have indicated the existence of synchronous divisions in the early stages of development of batch cultures (Houtermans, 1953;Ogur, Minckler & McClary, 1953 ;Browning, Brittain & Bergendahl, 1952). Yanagita (1956) suggested that cells a t stationary phases were physiologically synchronized, and when inoculated into fresh medium the initial phase of development was exhibited as synchronous growth. Takebe & Yanagita (1957) published curves showing stepwise development at the first generation of E. coli in peptone broth cultures derived from 24 and 48 h inocula.…”

Section: Synchronous Growthmentioning

confidence: 99%

“…On this theory synchronization would be expected whatever the source of 'shock' and whatever the phase of the growth cycle of the culture treated, and it is, therefore, not surprising that synchronization has been produced by temperature 'shock' (Hotchkiss, 1954;Lark & Maalrae, 1954;Szybalski & Hunter-Szybalska, 1955), by nutritional 'shock' (Barner & Cohen, 1956 ;Yanagita, 1954;Burns, 1959) and in Chlorella by light 'shock' (Tamiya et al, 1953). It follows that if size is related to age then inocula of cells of similar size should yield synchronously developing populations ; hence the great utility of the filtration technique of Maruyama & Yanagita (1956).…”

Section: Synchronous Growthmentioning

confidence: 99%

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A Genealogical Study of Clonal Development of Escherichia coli

Quesnel

1963

Journal of Applied Bacteriology

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The generation times of individual Escherichia coli cells inoculated on to a cellophane membrane surrounded by liquid synthetic medium in a continuous flow system were determined by direct observation for up to seven generations. The presence and production of nonviable cells was also recorded. A wide variety of developmental patterns was found among the developing clones; increased viability and a decrease in generation times with increasing age of the population were .observed. The phenomenon of alternation of generations is described and discussed. Five factors contributory to the lag phase were distinguished, and their relative effect may be deduced from the results. I 28

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Section: Synchronous Growthmentioning

confidence: 99%

Section: Synchronous Growthmentioning

confidence: 99%

A Genealogical Study of Clonal Development of Escherichia coli

Quesnel

1963

Journal of Applied Bacteriology

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“…Filtration procedure. The final filtration technique adopted was a modification of the procedure of Maruyama and Yanagita (20). Each of two 600-ml Pyrex Buchner-type funnels with a coarse fritted-glass disc, 9.0 cm in diameter, was packed with nine layers of Whatman no.…”

Section: Methodsmentioning

confidence: 99%

Synchronous Growth and Sporulation of Bacillus megaterium

1967

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Filtration of late log-phase cultures of Bacillus megaterium ATCC 19213 grown on defined sucrose salts medium (SS) or SS plus glutamate medium (SSG) through nine layers of Whatman no. 40 filter paper in a fritted-glass disc Buchner funnel resulted in filtrates containing cells which showed synchronous growth and proceeded to sporulation. SS cells completed one synchronous division after filtration; sporulation ensued after the cessation of growth. SSG cells completed two synchronous divisions and sporulation occurred during the second division. A high degree of synchrony of vegetative growth of SSG cells was evident by the stepwise pattern of growth, by the doubling of cell numbers at each division, the high division index, and by the rapid formation of sporulation cell types and homogeneity of cell types in the filtered cultures when compared with asynchronous cultures. Because the described system gives both good growth and sporulation synchrony, the method should be useful in delineating early events in sporulation and their regulation.

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“…The bacterial ''baby machine'' Charles Helmstetter working as a post-doc at the NIH developed the bacterial membrane-elution method in the early 1960s. (3,4) A few years earlier, Maruyama and Yanagita (5) had proposed a method for synchronizing cells where bacteria were sucked through a large pile of filter papers. The concept was that the smaller cells would percolate preferentially through the filter papers.…”

Section: Introductionmentioning

confidence: 99%

Minimally disturbed, multicycle, and reproducible synchrony using a eukaryotic “baby machine”

Cooper

2002

BioEssays

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A eukaryotic "baby machine" has been developed that produces synchronized cultures that display up to four synchronous cell cycles. That such cells can be produced implies that methods unable to produce successive synchronized cell cycles may not actually synchronize cells. But most important, the baby machine method now opens the way for the study of the cell cycle of minimally disturbed, artifact-free, well-synchronized, mammalian cells.

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PHYSICAL METHODS FOR OBTAINING SYNCHRONOUS CULTURE OF ESCHERICHIA COLI

Cited by 71 publications

References 10 publications

A Genealogical Study of Clonal Development of Escherichia coli

A Genealogical Study of Clonal Development of Escherichia coli

Synchronous Growth and Sporulation of Bacillus megaterium

Minimally disturbed, multicycle, and reproducible synchrony using a eukaryotic “baby machine”

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