Physical methods for genetic transformation of fungi and yeast

Rivera, Ana Leonor; Magaña-Ortíz, Denis; Gómez-Lim, Miguel Á.; Fernández, Francisco; Loske, Achim M.

doi:10.1016/j.plrev.2014.01.007

Cited by 54 publications

(38 citation statements)

References 267 publications

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“…This is due to its quick-ness, low cost, and simplicity even when it has a low efficiency, requires laborious protocols for regeneration after genetic transformation, and can only be applied to protoplasts [1,2,[12][13][14][15]. Electroporation is based on the application of a strong electrical field to enhance the formation of pores on the cell membrane due to a polarity alteration, caused by the electrical field (alternated or pulsed) that induces a dipolar moment inside the cells, and a potential difference through the plasmatic membrane [15][16][17][18].…”

Section: Electroporationmentioning

confidence: 99%

“…Biolistics consists on the acceleration of high density carrier particles (usually made of gold, tungsten or platinum) covered with genes that pass through the cells, leaving the DNA inside by an ad-sorption mechanism [1,2,35,36]. Cells, protoplasts, organized tissues like meristems, embryos or callus can be used as a target [37], and the introduction of multiple genes or chimeric DNA (DNA from two different species) is simple.…”

Section: Biolisticsmentioning

confidence: 99%

“…It can be performed without sophisticated devices and does not require chemical treatments or enzymatic cocktails. This technique is easy, cheap, and rivals electroporation for being the least time consuming methodology, but it is also one of the least efficient, because high quantities of DNA are damaged in the process and the viability of the cells is drastically reduced [2,47].…”

Section: Agitation With Glass Beadsmentioning

confidence: 99%

“…Several protocols are used to transfer DNA into cells of plants, fungi and [1,2]. Genetic transformation, discovered by Griffith [3] has revolutionized molecular biology, but it was not until the first recombinant DNA was produced from Escherichia coli with the use of bio-chemical scissors called restriction enzymes [4] that genetic transformation of cells started.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Transformation of Cells using Physical Methods

Loske¹

2014

J Genet Syndr Gene Ther

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The production of transgenic cells is a routinely process that allows to insert genes from plants, fungi, viruses, bacteria and even animals into cells. Genetic transformation requires penetration of the transgene through the cell wall. This process is facilitated by biological, chemical or physical methods. We present a short review of the state of the art of physical methods used for genetic transformation. A general panorama of the traditional physical genetic transformation methods like electroporation, biolistic, and agitation with glass beads, vacuum infiltration, silicon carbide whisker, laser microbeams, ultrasound, and the newly promising technique of shock wave-mediated genetic transformation of cells are described. These techniques have been applied to transform cells of bacteria, algae, plants, fungi, and animals. In human cells, genetic transformation is currently used for DNA vaccines, tissue engineering, and cancer therapy.

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Section: Electroporationmentioning

confidence: 99%

Section: Biolisticsmentioning

confidence: 99%

Section: Agitation With Glass Beadsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic Transformation of Cells using Physical Methods

Loske¹

2014

J Genet Syndr Gene Ther

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“…It has been employed successfully to transfer heterologous DNA into microbial cells (Calvin and Hanawalt 1988 ;Friedler and Wirth 1988 ;Miller et al 1988 ;Powell et al 1988 ;Dower et al 1988 ;Howard et al 1988 ;Mclntyre and Harlander 1989 ;Wen-Jun and Forde 1989 ;Theil and Poo 1989 ;Delorme 1989 ;Richley et al 1989 ;Hatterman and Stacey 1990 ), plant protoplasts (Shillito et al 1985 ;Fromm et al 1986 ;Riggs and Bates 1986 ;Toriyama et al 1988 ;Bellini et al 1989 ), and animal cells (Zerbib et al 1985 ;Narayanan et al 1986 ;Tur-Kaspa et al 1986 ;Chu et al 1987 ;Spandidos 1987 ;Knutson and Yee 1987 ;Hama-Inaba et al 1987 ). Extensive review on the process and strategies for fungal transformation is available (Fincham 1989 ;May 1992 ;Riach and Kinghorn 1996 ;Prasanna and Panda 1997 ;Ruiz-Diez 2002 ;Meyer 2008 ;Rivera et al 2012 ;Rivera et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%