Physical mapping of a pollen modifier locus controlling self-incompatibility in apricot and synteny analysis within the Rosaceae

Zuriaga, Elena; Molina, Laura; Badenes, M. L.; Romero, Carlos

doi:10.1007/s11103-012-9908-z

Cited by 24 publications

(35 citation statements)

References 58 publications

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“…(Perfection × Castleton) [76]SC [73]Mid-late f 13CastletonCEBASNAUSAPerfection × Newcastle [29]SC [76]Mid [41]14Ceglédi óriásSt. Istvan UEEHungaryUnknown (local selection) [16]SI [32]Mid [35]15ColoraoCEBASSE/NAfSpain (M)Unknown [41]↗ sterile [73]Mid [41]16CorbatóMAGRAMAWESpain (V)Unknown [25]?Mid-late [74]17Cow-1CAPAWEFranceINRA?Mid [35]18Cow-2CAPAWEFranceINRA??19CristalíFMWESpain (V)Unknown [25]?Mid [74]20CurrotIVIAWESpain (V)Unknown [25]SC [73]Early [74]21DulcineaCAPAWEItalyUnknown (Toscana variety)SC [73]Mid [35]22EffectSt. Istvan UEEUkraineKrupnolodnyi o.p.…”

Section: Resultsmentioning

confidence: 99%

“…[16]SC [16]Late [77]23EzzineCAPASE/NAfTunisiaINRAT?Early f 24FerganiSt. Istvan UEEFormer USSRUnknown??25Galta Roja a CAPAWESpain (V)Unknown [25]SC [73]Mid-early [41]26GVVFMWESpain (V)Unknown (isolated tree) [25]??27GandíaFMWESpain (V)Unknown [25]?Mid-early [74]28GavatxetFMWESpain (V)Unknown [25]?Mid [74]29GinestaIVIAWESpain (V)Unknown [25]SC e Mid-early [74]30GoldrichIVIANAUSASunglo × Perfection [76]SI [73]Mid [41, 47]31Gönci MagyarSt. Istvan UEEHungaryClone/hybrid?…”

Section: Resultsmentioning

confidence: 99%

“…In apricot, pollen-part mutations (PPMs) conferring SC by putatively affecting modifiers have been identified in the Spanish local cultivar ‘Canino’ and the North-American one ‘Katy’. Both PPMs were mapped at the distal end of chr.3 within the so-called M - and M ’-loci, respectively [25, 26]. Given their distinct geographic origins and the significant genetic distance estimated between both cultivars, it was initially hypothesized that these two PPMs could have arisen independently but affecting the same gene [26].…”

Section: Introductionmentioning

confidence: 99%

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Self-(in)compatibility in apricot germplasm is controlled by two major loci, S and M

et al. 2017

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BackgroundApricot (Prunus armeniaca L.) exhibits a gametophytic self-incompatibility (GSI) system and it is mostly considered as a self-incompatible species though numerous self-compatible exceptions occur. These are mainly linked to the mutated S C-haplotype carrying an insertion in the S-locus F-box gene that leads to a truncated protein. However, two S-locus unlinked pollen-part mutations (PPMs) termed m and m’ have also been reported to confer self-compatibility (SC) in the apricot cultivars ‘Canino’ and ‘Katy’, respectively. This work was aimed to explore whether other additional mutations might explain SC in apricot as well.ResultsA set of 67 cultivars/accessions with different geographic origins were analyzed by PCR-screening of the S- and M-loci genotypes, contrasting results with the available phenotype data. Up to 20 S-alleles, including 3 new ones, were detected and sequence analysis revealed interesting synonymies and homonymies in particular with S-alleles found in Chinese cultivars. Haplotype analysis performed by genotyping and determining linkage-phases of 7 SSR markers, showed that the m and m’ PPMs are linked to the same m 0−haplotype. Results indicate that m 0-haplotype is tightly associated with SC in apricot germplasm being quite frequent in Europe and North-America. However, its prevalence is lower than that for S C in terms of frequency and geographic distribution. Structures of 34 additional M-haplotypes were inferred and analyzed to depict phylogenetic relationships and M 1–2 was found to be the closest haplotype to m 0. Genotyping results showed that four cultivars classified as self-compatible do not have neither the S C- nor the m 0-haplotype.ConclusionsAccording to apricot germplasm S-genotyping, a loss of genetic diversity affecting the S-locus has been produced probably due to crop dissemination. Genotyping and phenotyping data support that self-(in)compatibility in apricot relies mainly on the S- but also on the M-locus. Regarding this latter, we have shown that the m 0-haplotype associated with SC is shared by ‘Canino’, ‘Katy’ and many other cultivars. Its origin is still unknown but phylogenetic analysis supports that m 0 arose later in time than S C from a widely distributed M-haplotype. Lastly, other mutants putatively carrying new mutations conferring SC have also been identified deserving future research.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-1027-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Self-(in)compatibility in apricot germplasm is controlled by two major loci, S and M

et al. 2017

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“…Interestingly, the modifier loci of sweet cherry and apricot cultivars were located at the distal end of linkage group 3 (M locus), which is the syntenic region of the Maleae S locus Wünsch, 2011, 2014;Zuriaga et al, 2012Zuriaga et al, , 2013. However, none of genes reported to be involved in SI are located in this region (Habu and Tao, 2013;Zuriaga et al, 2012). Identifying the pollen modifier gene will provide new insights into the Prunus-specific SI mechanism.…”

Section: Self-incompatibility Events That Arrest Self-pollen Tube Gromentioning

confidence: 99%

Distinct Self-recognition in the <i>Prunus</i> S-RNase-based Gametophytic Self-incompatibility System

Matsumoto

Tao

2016

Hortic J

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Prunus fruit tree species exhibit S-ribonuclease (S-RNase)-based gametophytic self-incompatibility (GSI). This system is also present in the families Plantaginaceae and Solanaceae and the tribe Maleae of the family Rosaceae. In S-RNase-based GSI, self/nonself-recognition between the pistil and pollen is controlled by the pistil S determinant S-ribonuclease gene (S-RNase) and the pollen S determinant F-box gene(s). Accumulated evidence indicates the Prunus pollen S locus contains a single F-box gene, while that of other plants consists of multiple F-box genes. The pollen S F-box genes are called S haplotype-specific F-box (SFB), S-locus F-box brothers (SFBB), and S-locus F-box (SLF) in Prunus, Maleae, and Solanaceae species, respectively. The consequences of pollen S gene mutations and heterodiallelic pollen production differ between Prunus species and other plants, suggesting there are different pollen S functions during self/nonself-recognition. The GSI systems of Prunus and other plants are believed to include the ubiquitin proteasome system for protein degradation. However, Prunus SFB is assumed to facilitate the S-RNase cytotoxic effects during selfrecognition, while SLFs and SFBBs are thought to function collaboratively during nonself-recognition to avoid S-RNase cytotoxicity. This review summarizes the distinct features of the S-RNase-based GSI mechanism in Prunus species, with special references to the recent advances in our understanding of S-RNase-based GSI.

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“…3 flanked by two SSR markers within an interval of 1.8 cM, corresponding to ~364 Kb in the peach genome (Zuriaga et al, 2012), we searched for differentially expressed unigenes located at this locus by MEGABLAST searching against the peach genome (The International Peach Genome Initiative, 2013). We found 19 unigenes pollen and pistils or up-regulated at least either in pollen or pistils because they were found in both the UP-and NKP-specific unigenes, while the expressions of 432 and 301 unigenes were up-regulated specifically in pollen and pistils, respectively.…”

Section: Searching For Modifier Genes In Prunusmentioning

confidence: 99%

Transcriptome Analysis of Self- and Cross-pollinated Pistils of Japanese Apricot (Prunus mume Sieb. et Zucc.)

Habu

Tao

2014

J. Japan. Soc. Hort. Sci.

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Solanaceae, Rosaceae, and Plantaginaceae exhibit the S-RNase-based gametophytic self-incompatibility (GSI) system. This type of GSI is controlled by a single polymorphic locus (S locus) containing the pistil S determinant gene, S-ribonuclease (S-RNase), and the pollen S determinant, the S locus F-box gene (SFB/SLF). In addition to these determinant genes, non-S factors, called modifier genes, are required for the GSI reaction. Here, we conducted large-scale transcriptome analysis of unpollinated, self-pollinated, and cross-pollinated pistils of Japanese apricot (Prunus mume Sieb. et Zucc. cv. Nanko) to capture all of the molecular events induced by the GSI reaction in Prunus, using next-generation sequencing technologies. We obtained 40,061 unigenes from 77,521,310 reads from pollinated and unpollinated pistils and pollen grains. Among these unigenes, 29,985 and 27,898 unigene sequences showed at least one hit against the NCBI nr and TAIR10 protein databases, respectively, in BLASTX searches using an E-value cutoff of 1e-6. Digital expression analysis showed that 8,907 and 10,190 unigenes were expressed at significantly different levels between unpollinated (UP) and cross-pollinated (CP) pistils and between UP and self-pollinated (SP) pistils, respectively. The expression of 4,348 unigenes in both CP and SP pollination was commonly and significantly different from that in UP, while the expression of 4,559 and 5,842 unigenes in CP and SP, respectively, was specifically and significantly different from UP. The expression of 2,227 unigenes was up-regulated both in CP and SP compared with UP. Genes supposedly involved in S-RNasebased GSI were included among the unigenes up-regulated by pollination, while no unigenes homologous to the solanaceous pistil modifiers HT-B or 120K were included among the unigenes up-regulated by pollination or in the whole unpollinated/pollinated pistil transcriptome. We discuss the distinct molecular mechanism of S-RNase-based GSI in Prunus.

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Physical mapping of a pollen modifier locus controlling self-incompatibility in apricot and synteny analysis within the Rosaceae

Cited by 24 publications

References 58 publications

Self-(in)compatibility in apricot germplasm is controlled by two major loci, S and M

Self-(in)compatibility in apricot germplasm is controlled by two major loci, S and M

Distinct Self-recognition in the <i>Prunus</i> S-RNase-based Gametophytic Self-incompatibility System

Transcriptome Analysis of Self- and Cross-pollinated Pistils of Japanese Apricot (Prunus mume Sieb. et Zucc.)

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