Physical mapping and homology studies of egg drop syndrome (EDS-76) adenovirus DNA

Abstract

Homologous sequences between EDS-76 adenovirus strain 127 DNA and bovine adenovirus DNA were found by the Southern blotting technique and no homology with CELO virus DNA could be detected. These data suggest a genetic similarity between EDS-76 virus and bovine adenoviruses. The fragments generated form EDS-76 adenovirus DNA by eight restriction endonucleases were physically mapped.

DNA sequencing and analysis of the right-hand part of the genome of the unique bovine adenovirus type 10

“…From the previously cloned ClaI fragments of the Belfast1 genome (Matiz et al, 1998), subclones were prepared by cleavage with ApaI, BamHI and HindIII restriction enzymes followed by ligation in pBluescript SK phagemid (Stratagene). The terminal fragments of the genome were cloned using alkaline treatment to remove possible traces of the terminal protein remaining at the 59 ends (Zakharchuk et al, 1993). In the clones encompassing the right-hand part of the genome, nested deletions were prepared with exonuclease III treatment and religation (ExoIII/S1 Deletion Kit; MBI Fermentase).…”

“…Officially, ten bovine adenovirus (BAdV) types are recognized , but at least one additional candidate (isolate Rus) has already been partially characterized (Zakharchuk et al, 1993;É lő, 2002). Thus, after the human and fowl adenoviruses (HAdVs and FAdVs), BAdVs comprise the third largest group of adenoviruses originating from one host species.…”

Characterization of the haemorrhagic enteritis virus genome and the sequence of the putative penton base and core protein genes

“…Secondly, the FAdV-10 penton base gene had a G+C content of 59"8%, while that of the HEV target DNA was 33.8%, a disparity that would be expected to lead to a high background and a lack of hybridization. Zakharchuk et al (1993) performed Southern hybridization experiments with egg drop syndrome 1976 virus (EDS-76), an avian adenovirus with a 34.2 kb genome and one fibre per penton base. They found DNA sequence similarity between EDS-76 and bovine adenoviruses, but found no similarity, under low stringency conditions, with either FAdV-1 (chick embryo lethal orphan virus, CELO) or HAdV-5.…”