Physical map of Campylobacter jejuni TGH9011 and localization of 10 genetic markers by use of pulsed-field gel electrophoresis

Abstract

The physical map of Campylobacter jejuni TGH9011 (ATCC 43430) was constructed by mapping the three restriction enzyme sites SacII (CCGCGG), SalI (GTCGAC), and SmaI (CCCGGG) on the genome of C. jejuni by using pulsed-field gel electrophoresis and Southern hybridization. A total of 25 restriction enzyme sites were mapped onto the C. jejuni chromosome. The size of the genome was reevaluated and was shown to be 1,812.5 kb. Ten C. jejuni genetic markers that have been isolated in our laboratory were mapped to specific restriction enzyme fragments. Furthermore, we have accurately mapped one of the three rRNA operons (rrnA) and have demonstrated a separation of the 16S and 23S rRNA-encoding sequences in one of the rRNA operons.

Identification ofCampylobacter jejuniATCC 43431-Specific Genes by Whole Microbial Genome Comparisons

“…This allowed us to identify strain-specific diversity which might be involved in virulence potential. These two C. jejuni strains differ by several phenotypic and genotypic features: (i) their Penner serotype: ATCC 43431 (TGH 9011) is Penner serotype O:3, while NCTC 11168 is Penner serotype O:2 (49); (ii) their invasion ability: NCTC 11168 is poorly invasive (3), while ATCC 43431 appears to be highly invasive (17,18); (iii) their genome size: the genome of ATCC 43431 appears to be larger than that of NCTC 11168 (21,34); and (iv) their gene order: gene positions seem to differ considerably between the two strains (21,22,34). Since invasion ability has been shown to correlate with epidemiological outcomes (10), the genes unique to ATCC 43431 will provide a valuable resource to dissect the C. jejuni features that affect pathogenic potential.…”

Phylogenetic and molecular characterization of a 23S rRNA gene positions the genus Campylobacter in the epsilon subdivision of the Proteobacteria and shows that the presence of transcribed spacers is common in Campylobacter spp

“…Typically, these small, nutritionally fastidious, microaerophilic, gram-negative spiral bacteria colonize moist body surfaces, including the human gingival cavity, the small intestinal mucosa, and the vaginal mucosa of bovines and this colonization leads to disease. The two most important human pathogens in the genus are Campylobacterjejuni and C. coli, which cause enteritis.In keeping with their small size and limited nutritional properties, the campylobacters have a small genome (23,37,57). At 1.7 to 1.8 Mb, the C. jejuni and C. coli genomes are only 36% of the size of the Escherichia coli chromosome (53) (23,24,27,37,57).…”

“…In keeping with their small size and limited nutritional properties, the campylobacters have a small genome (23,37,57). At 1.7 to 1.8 Mb, the C. jejuni and C. coli genomes are only 36% of the size of the Escherichia coli chromosome (53) (23,24,27,37,57).…”

“…While E. coli has seven rRNA genomic loci (26), C. jejuni has only three copies of the 16S and 23S rRNA genes (23,24,27,37,57). Unlike the characteristic continuous 16S-23S (eu)bacterial rRNA operon structure seen in E. coli (35), several studies have suggested that in at least one of the three C. jejuni rRNA operons, the 16S and 23S genes are located separately (23,27,37,57). However, recent fine mapping of the three rRNA gene loci of one of these C. jejuni strains has shown that all three operons possess a contiguous 16S-23S structure (25).…”

An isoleucyl-tRNA synthetase gene from Campylobacter jejuni

“…The ileS gene was mapped by Southern hybridization using a "Plabelled 2 kb HindIII fragment of E8-1 as the probe. The C. jtjzini genome was digested with SmaI, SaA and Sac11 and fragments were resolved by PFGE as described by Kim et al (1992).…”