Physical manipulation of single-molecule DNA using microbead and its application to analysis of DNA–protein interaction

Kurita, Hirofumi; Yasuda, Hachiro; Takashima, Kazunori; Katsura, Shinji; Mizuno, Akira

doi:10.1016/j.jmmm.2008.11.018

Cited by 1 publication

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure 3 demonstrates that one end of a single DNA molecule is immobilized on the surface, whereas the other end is attached to a particle. The manipulation of the particle under this condition enables the uniform stretching of the trapped single DNA molecules [ 50 , 51 ]. The use of dual-beam optical tweezers can successfully stretch DNA molecules whose termini are attached to different particles and control their tension based on the particle position [ 52 , 53 ].…”

Section: Dna Manipulationmentioning

confidence: 99%

DNA Manipulation and Single-Molecule Imaging

2021

Self Cite

View full text Add to dashboard Cite

DNA replication, repair, and recombination in the cell play a significant role in the regulation of the inheritance, maintenance, and transfer of genetic information. To elucidate the biomolecular mechanism in the cell, some molecular models of DNA replication, repair, and recombination have been proposed. These biological studies have been conducted using bulk assays, such as gel electrophoresis. Because in bulk assays, several millions of biomolecules are subjected to analysis, the results of the biological analysis only reveal the average behavior of a large number of biomolecules. Therefore, revealing the elementary biological processes of a protein acting on DNA (e.g., the binding of protein to DNA, DNA synthesis, the pause of DNA synthesis, and the release of protein from DNA) is difficult. Single-molecule imaging allows the analysis of the dynamic behaviors of individual biomolecules that are hidden during bulk experiments. Thus, the methods for single-molecule imaging have provided new insights into almost all of the aspects of the elementary processes of DNA replication, repair, and recombination. However, in an aqueous solution, DNA molecules are in a randomly coiled state. Thus, the manipulation of the physical form of the single DNA molecules is important. In this review, we provide an overview of the unique studies on DNA manipulation and single-molecule imaging to analyze the dynamic interaction between DNA and protein.

show abstract