Physical localization and genetic mapping of the fertility restoration gene<i>Rfo</i>in canola (<i>Brassica napus</i>L.)

Feng, Jiuhuan; Primomo, Valerio; Li, Zenglu LiZ.; Zhang, Yongping ZhangY.; Jan, Chao-Chien JanC.-C.; Tulsieram, Lomas; Xu, Steven S.

doi:10.1139/g09-016

Cited by 21 publications

(20 citation statements)

References 31 publications

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“…BAC clones frequently contain many repetitive sequences which are homologous in the target genome, requiring high stringencies and blocking in FISH (Kim et al 2002;Schwarzacher 2008) to identify loci of the specific traits linked to that BAC clone. Similar clear BAC-FISH signal results were also found by Feng et al (2009) when localizing the Rfo gene in oilseed rape genome using two different R. sativus BAC probes (G62 and B420) linked to the Rfo locus.…”

Section: Discussionsupporting

confidence: 79%

“…To achieve complete stability of the Rfk1 gene, also exploited in practical breeding, the integration of the gene to the A genome chromosomes of turnip rape may be required. The Rfo gene, which is stably integrated in the oilseed rape C genome (Hu et al 2008;Feng et al 2009), is now used for hybrid seed production (Budar et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…This approach has successfully been used to detect different genomes in interspecific hybrids as well as for locating introgressed genomes, alien chromosomes, or chromosomal segments in another genomic background (Schwarzacher et al 1992). The recent progress in Brassica genome-sequencing projects have provided useful sequence information for cytogenetic studies (for review, see Heslop-Harrison and Schwarzacher 2011) and Brassica BAC clones have been used for hybridization as FISH probes to physically localize specific sequences on Brassica chromosomes (Howell et al 2005(Howell et al , 2008Nicolas et al 2007Nicolas et al , 2008Feng et al 2009;Kim et al 2009;Szadkowski et al 2010Szadkowski et al , 2011Xiong and Pires 2010;Xiong et al 2011). As in most plant species, transposable elements are abundant in the Brassica genome, and some of these are genome-specific (Alix et al 2005(Alix et al , 2008Lim et al 2007); there are also tandemly repeated deoxyribonucleic acid (DNA) motifs which show specificity to some chromosomes and genomes (Harrison and Heslop-Harrison 1995).…”

Section: Introductionmentioning

confidence: 99%

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Size and location of radish chromosome regions carrying the fertility restorer Rfk1 gene in spring turnip rape

et al. 2012

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In spring turnip rape (Brassica rapa L. spp. oleifera), the most promising F1 hybrid system would be the Ogu-INRA CMS/Rf system. A Kosena fertility restorer gene Rfk1, homolog of the Ogura restorer gene Rfo, was successfully transferred from oilseed rape into turnip rape and that restored the fertility in female lines carrying Ogura cms. The trait was, however, unstable in subsequent generations. The physical localization of the radish chromosomal region carrying the Rfk1 gene was investigated using genomic in situ hybridization (GISH) and bacterial artificial chromosome-fluorescence in situ hybridization (BAC-FISH) methods. The metaphase chromosomes were hybridized using radish DNA as the genomic probe and BAC64 probe, which is linked with Rfo gene. Both probes showed a signal in the chromosome spreads of the restorer line 4021-2 Rfk of turnip rape but not in the negative control line 4021B. The GISH analyses clearly showed that the turnip rape restorer plants were either monosomic (2n=2x=20+1R) or disomic (2n=2x=20+2R) addition lines with one or two copies of a single alien chromosome region originating from radish. In the BAC-FISH analysis, double dot signals were detected in subterminal parts of the radish chromosome arms showing that the fertility restorer gene Rfk1 was located in this additional radish chromosome. Detected disomic addition lines were found to be unstable for turnip rape hybrid production. Using the BAC-FISH analysis, weak signals were sometimes visible in two chromosomes of turnip rape and a homologous region of Rfk1 in chromosome 9 of the B. rapa A genome was verified with BLAST analysis. In the future, this homologous area in A genome could be substituted with radish chromosome area carrying the Rfk1 gene.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Size and location of radish chromosome regions carrying the fertility restorer Rfk1 gene in spring turnip rape

et al. 2012

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“…tritici Em. Marchal) resistance transferred into wheat, and Rfo for fertility restoration transferred from radish (Raphanus sativus L.) into canola (Brassica napus L.) (Feng et al 2009). The end products of most chromosome engineering studies have been chromosome additions or translocations involving large alien chromosomal segments (see reviews by Jiang et al 1994;Friebe et al 1996;Fedak 1999;Xu et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Targeted Introgression of a Wheat Stem Rust Resistance Gene by DNA Marker-Assisted Chromosome Engineering

et al. 2011

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Chromosome engineering is a useful strategy for transfer of alien genes from wild relatives into modern crops. However, this strategy has not been extensively used for alien gene introgression in most crops due to low efficiency of conventional cytogenetic techniques. Here, we report an improved scheme of chromosome engineering for efficient elimination of a large amount of goatgrass (Aegilops speltoides) chromatin surrounding Sr39, a gene that provides resistance to multiple stem rust races, including Ug99 (TTKSK) in wheat. The wheat ph1b mutation, which promotes meiotic pairing between homoeologous chromosomes, was employed to induce recombination between wheat chromosome 2B and goatgrass 2S chromatin using a backcross scheme favorable for inducing and detecting the homoeologous recombinants with small goatgrass chromosome segments. Forty recombinants with Sr39 with reduced surrounding goatgrass chromatin were quickly identified from 1048 backcross progenies through disease screening and molecular marker analysis. Four of the recombinants carrying Sr39 with a minimal amount of goatgrass chromatin (2.87-9.15% of the translocated chromosomes) were verified using genomic in situ hybridization. Approximately 97% of the goatgrass chromatin was eliminated in one of the recombinants, in which a tiny goatgrass chromosome segment containing Sr39 was retained in the wheat genome. Localization of the goatgrass chromatin in the recombinants led to rapid development of three molecular markers tightly linked to Sr39. The new wheat lines and markers provide useful resources for the ongoing global effort to combat Ug99. This study has demonstrated great potential of chromosome engineering in genome manipulation for plant improvement.

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“…The first improvement is the application of chromosome-specific bacterial artificial chromosome (BAC) probes and new repetitive sequences, which has facilitated chromosome identification in B. rapa and B. oleracea (Howell et al 2002(Howell et al , 2008Koo et al 2004;Lim et al 2005Lim et al , 2007Mun et al 2008;Feng et al 2009;Kim et al 2009). Howell et al (2002) made a first step toward integration of all nine linkage groups of the B. oleracea genetic map to the corresponding chromosomes using BACs as probes for FISH hybridization.…”

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confidence: 99%

Karyotype and Identification of All Homoeologous Chromosomes of AllopolyploidBrassica napusand Its Diploid Progenitors

2011

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Investigating recombination of homoeologous chromosomes in allopolyploid species is central to understanding plant breeding and evolution. However, examining chromosome pairing in the allotetraploid Brassica napus has been hampered by the lack of chromosome-specific molecular probes. In this study, we establish the identification of all homoeologous chromosomes of allopolyploid B. napus by using robust molecular cytogenetic karyotypes developed for the progenitor species Brassica rapa (A genome) and Brassica oleracea (C genome). The identification of every chromosome among these three Brassica species utilized genetically mapped bacterial artificial chromosomes (BACs) from B. rapa as probes for fluorescent in situ hybridization (FISH). With this BAC-FISH data, a second karyotype was developed using two BACs that contained repetitive DNA sequences and the ubiquitous ribosomal and pericentromere repeats. Using this diagnostic probe mix and a BAC that contained a C-genome repeat in two successive hybridizations allowed for routine identification of the corresponding homoeologous chromosomes between the A and C genomes of B. napus. When applied to the B. napus cultivar Stellar, we detected one chromosomal rearrangement relative to the parental karyotypes. This robust novel chromosomal painting technique will have biological applications for the understanding of chromosome pairing, homoeologous recombination, and genome evolution in the genus Brassica and will facilitate new applied breeding technologies that rely upon identification of chromosomes.

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Physical localization and genetic mapping of the fertility restoration geneRfoin canola (Brassica napusL.)

Cited by 21 publications

References 31 publications

Size and location of radish chromosome regions carrying the fertility restorer Rfk1 gene in spring turnip rape

Size and location of radish chromosome regions carrying the fertility restorer Rfk1 gene in spring turnip rape

Targeted Introgression of a Wheat Stem Rust Resistance Gene by DNA Marker-Assisted Chromosome Engineering

Karyotype and Identification of All Homoeologous Chromosomes of AllopolyploidBrassica napusand Its Diploid Progenitors

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