Physical Linkage of the B29/Ig-β (CD79B) Gene to the Skeletal Muscle, Sodium-Channel, and Growth Hormone Genes in Rat and Human

Nakazato, Satomi; Nomoto, Keiko; Kazahari, Koji; Ono, M.

doi:10.1006/geno.1997.5178

Cited by 11 publications

(18 citation statements)

References 26 publications

(3 reference statements)

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“…In chickens, the sodium channel gene and the GH gene are present in the upstream and downstream regions of the Ig-b gene, respectively, with the same transcriptional orientations as in rat and humans (Nakazato et al, 1998). However, the distance between these genes is shorter in chickens when compared to the mammalian homologs ( Fig.…”

Section: Discussionmentioning

confidence: 94%

DNase I hypersensitive sites and histone acetylation status in the chicken Ig-β locus

Murakami¹,

Osano²,

Ono³

2004

Gene

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Section: Discussionmentioning

confidence: 94%

DNase I hypersensitive sites and histone acetylation status in the chicken Ig-β locus

Murakami¹,

Osano²,

Ono³

2004

Gene

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“…Complete sequencing of the two overlapping clones indicated that the 5′ end was 0.5 kb shorter than rat TCAM-1 cDNA (Ono et al 1999). Mouse TCAM-1 cDNA clones extending to the 5′ end were prepared with 5′ RACE Systems (Nakazato et al 1998). The second nested PCR with AUAP at the 5′ end and P3 ( Fig.…”

Section: Cloning Of Mouse Tcam-1 Cdnamentioning

confidence: 99%

“…2. Using the LA PCR kit (Takara, Japan), PCR was carried out with kidney DNA from male C3H mouse as template (Nakazato et al 1998) and amplified DNA was subcloned into pCR2.1-TOPO.…”

Section: ′ Race and La Pcrmentioning

confidence: 99%

Structure, expression, and conserved physical linkage of mouse testicular cell adhesion molecule-1 (TCAM-1) gene

Sakatani¹,

Takahashi²,

Okuda³

et al. 2000

Genome

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Isolation and characterization were performed for cDNA encoding mouse testicular cell adhesion molecule-1 (TCAM-1) using 2908 bases coding for a protein having 548 amino acids (60 kDa). Mouse TCAM-1 protein was found to consist of seven domains for signal sequence, five immunoglobulin (Ig) domains, and the transmembrane plus cytoplasmic domain. TCAM-1 gene and the region linking it to growth hormone (GH) gene located downstream from the TCAM-1 gene were then analyzed. The mouse TCAM-1 gene was 11.6 kb in length with 8 exons; the same as for the 12.0 kb rat gene. The distance from the TCAM-1 to GH gene was 12.5 kb in the mouse genome, and 7.6 kb in the rat. By Northern hybridization, 3.1-kb TCAM-1 mRNA was detected in 17-day testis and would appear present in pachytene spermatocytes and round spermatids.

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“…3 The rat, mouse, and human B29 promoters contain highly conserved sequences with a core of essentially identical transcription factor binding motifs. [4][5][6] The B29 promoter in each species has an essential octamer motif whose consensus is competent for Bob1 (OCA-B, OBF-1) co-activator binding. 7,8 Rat B29 enhancers, designated DNase 1 hypersensitive sites (DHS) +4.4, +6.0, and +8.7, were identified downstream of B29 in the intergenic region between the B29 and the growth hormone (GH) genes.…”

Section: Introductionmentioning

confidence: 99%

B29 Gene Silencing in Pituitary Cells Is Regulated by Its 3′ Enhancer

Malone

Kuraishy

Fike

et al. 2006

Journal of Molecular Biology

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B cell-specific B29 (Igbeta, CD79b) genes in rat, mouse, and human are situated between the 5' growth hormone (GH) locus control region and the 3' GH gene cluster. The entire GH genomic region is DNase 1 hypersensitive in GH-expressing pituitary cells, which predicts an "open" chromatin configuration, and yet B29 is not expressed. The B29 promoter and enhancers exhibit histone deacetylation in pituitary cells, but histone deacetylase inhibition failed to activate B29 expression. The B29 promoter and a 3' enhancer showed local dense DNA methylation in both pituitary and non-lymphoid cells consistent with gene silencing. However, DNA methyltransferase inhibition did not activate B29 expression either. B29 promoter constructs were minimally activated in transfected pituitary cells. Co-transfection of the B cell-specific octamer transcriptional co-activator Bob1 with the B29 promoter construct resulted in high level promoter activity in pituitary cells comparable to B29 promoter activity in transfected B cells. Unexpectedly, inclusion of the B29 3' enhancer in B29 promoter constructs strongly inhibited B29 transcriptional activity even when pituitary cells were co-transfected with Bob1. Both Oct-1 and Pit-1 bind the B29 3' enhancer in in vitro electrophoretic mobility shift assay and in in vivo chromatin immunoprecipitation analyses. These data indicate that the GH locus-embedded, tissue-specific B29 gene is silenced in GH-expressing pituitary cells by epigenetic mechanisms, the lack of a B cell-specific transcription factor, and likely by the B29 3' enhancer acting as a powerful silencer in a context and tissue-specific manner.

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Physical Linkage of the B29/Ig-β (CD79B) Gene to the Skeletal Muscle, Sodium-Channel, and Growth Hormone Genes in Rat and Human

Cited by 11 publications

References 26 publications

DNase I hypersensitive sites and histone acetylation status in the chicken Ig-β locus

DNase I hypersensitive sites and histone acetylation status in the chicken Ig-β locus

Structure, expression, and conserved physical linkage of mouse testicular cell adhesion molecule-1 (TCAM-1) gene

B29 Gene Silencing in Pituitary Cells Is Regulated by Its 3′ Enhancer

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