Physical linkage of a guanine nucleotide-binding protein-related gene to the chicken major histocompatibility complex.

Abstract

Several genes were found closely associated with major histocompatibility class I and class I ,B-chain genes in chicken genomic DNA clusters by hybridizing tissue-specific cDNA probes to cosmid clones. A cDNA probe for one of these genes, probe C12.3 isolated from a chicken liver cDNA library, was used to clone the homologous sequence H12. C12.3-(536-516), shown as boxes in Fig. 1B, and the M13 universal primer were used as sequencing primers. Oligonucleotide synthesis has been described (7). Nucleotide sequences were compiled by using the PCGENE software package (Genofit, Geneva). The 12.3 amino acid sequence* was searched against the GenBank protein sequence data base (Bolt, Beranek, and Newman) by using the program KANEHISA (9) in the Bisance facility (CITI2, Paris). The scores of alignments between 12.3 and guanine nucleotidebinding protein (G protein) ,B subunit 2 (GPB2) sequences, given in SD units, were obtained with the ALIGN program (10)

Three‐dimensional fibroblast–tumor cell interaction causes downregulation of RACK1 mRNA expression in breast cancer cells in vitro

“…Other synonyms for the protein are H12.3 and Rack1 (receptor for activated protein C Kinase 1). 14 cDNA cloned from BT474 monoculture RAP-PCR was 100% homologous to nucleotides 385-844 of the rack1 mRNA sequence 15 (Fig. 2b).…”

“…GNB2L1 is a member of the superfamily of heterologous G-protein ␤ subunits, which are characterized by highly conserved internal WD40 repeats (Trp-Asp 15 ). The function of Rack1 is not well understood.…”

Cloning of an intracellular receptor for protein kinase C: a homolog of the beta subunit of G proteins.

“…The nitrocellulose pieces (containing PKC/RACK1 complexes) were incubated with sample buffer and subjected to SDS/PAGE to elute the bound proteins. Western [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36] were synthesized at the Biomolecular Resource Center, University of California, San Francisco. The purity and structural integrity of the peptides were confirmed by HPLC, amino acid analysis, and fast-atom bombardment mass spectroscopy.…”

The WD‐40 repeat

“…Members of the WD-40 family include: (i) the fl-subunit of guanine nucleotide regulatory proteins (G-proteins), a component of the heterotrimeric complex that transduces signals from transmembrane receptors to a variety of second messenger generating effectors [1,2]; (ii) STE4, a functional G-protein fl-subunit homologue in yeast (Saccharomyces cerevisiae), involved in a signal pathway that controls the response to mating pheromone [3]; (iii) CDC4, a component of the yeast nuclear cytoskeleton, required at the late Gj/S phase boundary of the cell cycle [4,5]; (iv) CDC20, a yeast gene product required for several microtubule-dependent processes at multiple stages in the cell cycle [6][7][8] [9]; (vi) I2.3, the product of a gene in the chicken MHC-iocus [10]; (vii) PRP4, a stable component of yeast U4/U6 small nuclear ribonucleoprotein particle (snRNP) [11][12][13]; (viii) PRPI7, another protein involved in pre-mgNA splicing in yeast [13]; (ix) AER~ TUP1, a transcriptional repressor in yeast [14--15]; (x) MSll, a negative regulator of the RAS-cAMP pathway in yeast [16]; (xi) coronin, a component of the actin/ myosin complex of the slimemoid Dict),ostelium discoideum [17]; (xii) PWPI, a yeast protein, containing periodic tryptophan residues [18]; (xiii) Clbp, a Chlano,do. monas protein of unknown function [19]; (xiv) MAKI 1, the apparently membrane associated product of an essential yeast gene, necessary for the maintenance of killer M1 double-stranded RNA [20]; and (xv) AAC3, the deduced product of a developmentally regulated transcript in Dictyosteliunz discoideum that contains long AAC repeats (reading fi'ame specifies glutamine in this case) [21].…”