Physical interaction with monocytes rescues human mature CD4+ T-cell lines from anti-CD3-induced apoptosis

Yamauchi, Yoriko; Katamura, Kenji; Shimizu, N; Fukui, Tetsuya; Ohshima, Yusei; Mayumi, Mitsufumi; Furusho, Kensi

doi:10.1016/0165-2478(95)00025-z

Cited by 2 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The data also suggested that the CD14 cocktails could rescue activated lymphocytes from undergoing AICD. However, data from other laboratories have shown no effect on apoptosis of soluble factors from activated monocytes 28,29 . This might be, in part, a result of the different systems used.…”

Section: Discussionmentioning

confidence: 94%

“…However, data from other laboratories have shown no effect on apoptosis of soluble factors from activated monocytes. 28,29 This might be, in part, a result of the different systems used. The previously used system was a classic two-chamber system.…”

Section: Discussionmentioning

confidence: 99%

“…There are contrasting data on the role of monocytes in lymphocyte apoptosis. While monocytes were reported to rescue activated lymphocytes from spontaneous apoptosis, 28,29 other laboratories reported that monocytes were required to prime T cells to undergo apoptosis 30 . Soluble factors from activated monocytes were reported to have no effect on apoptosis 28,29 …”

Section: Introductionmentioning

confidence: 99%

“…30 Soluble factors from activated monocytes were reported to have no effect on apoptosis. 28,29 Professional antigen-presenting cells (APCs) can trigger immune responses. APCs include monocytes, macrophages, dendritic cells and B cells.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Characterizing a soluble survival signal for activated lymphocytes from CD14⁺ cells

et al. 2002

View full text Add to dashboard Cite

SUMMARYT-cell activation requires at least two signals: antigen and a costimulatory signal. As antigenpresenting cells play an important role in this area, the role of CD14+ cells in T-cell activation, proliferation and activation-induced cell death (AICD) was investigated. Using phytohaemagglutinin (PHA) activation, it was found that CD14 + cell depletion resulted in significantly greater AICD, decreased lymphocyte growth and up-regulated interleukin-2 (IL-2) secretion. However, T-cell activation was delayed according to the expression of CD69 and CD25. Dynabeads conjugated with anti-CD14 monoclonal antibody (mAb) bound CD14 + cells and induced secretion of IL-1b, tumour necrosis factor-a (TNF-a), transforming growth factor-b (TGF-b) and IL-6, but not IL-2, IL-12 or IL-15. Supernatants were collected from Dynabeads-activated CD14 + cell cultures and designated as 'CD14 cocktails'.Addition of CD14 cocktails to CD14 + cell-depleted mononuclear cell cultures reversed the increased AICD, decreased lymphocyte growth and increased IL-2 secretion. Depletion of IL-1b and TNF-a in the CD14 cocktails by panning followed by blocking with the corresponding mAbs had no effect on the active AICD protection. TGF-b was determined not to be the active factor owing to the presence of >1 . 0 ng of TGF-b in the media for culturing both CD14 + and CD14 x peripheral blood mononuclear cells (PBMC). The CD14 cocktails did not contain IL-12 and IL-15. Depletion of IL-6 with panning followed by blocking residual IL-6 with anti-IL-6 mAb significantly reduced the protective effect of the CD14 cocktails. Human recombinant IL-6 also partially reversed the effects of CD14 + cell depletion on AICD, lymphocyte growth and IL-2 secretion. The data suggest that IL-6 is one of the active factors in the survival signal from CD14 + cells.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterizing a soluble survival signal for activated lymphocytes from CD14⁺ cells

et al. 2002

View full text Add to dashboard Cite

show abstract

“…Conversely, accessory cells can prime T cells to receive an apoptotic signal. For example, activated human monocytes can prime peripheral blood T cells to undergo apoptosis [21], whereas nonactivated monocytes provide a protective signal to T cells [22]. In either situation, physical contact is needed to bring about their effect.…”

mentioning

confidence: 99%

Monocyte Rescue of Human T Cells from Apoptosis is CD40/CD154 Dependent

Sakata

Kong

et al. 1999

Scand J Immunol

View full text Add to dashboard Cite

The induction of T-cell apoptosis is regulated in part by monocytes (CD14+ cells). Human peripheral blood monocytes inhibited the spontaneous cell death of activated T cells in vitro. The inhibition of T-cell apoptosis did not require autologous monocytes. Inhibition required direct contact with monocytes and was not due to a soluble factor. Furthermore, treatment of monocytes with actinomycin D, cycloheximide and paraformaldehyde abrogated the anti-apoptotic activity of these cells. Blocking antibody to CD40 and CD154 (CD40 ligand) decreased the ability of monocytes to aid in T-cell survival, whereas, blocking LFA-1/I-CAM-1, Fas ligand and the CD4/major histocompatibility complex class II interaction did not affect the influence of monocytes on T-cell survival. This shows that monocytes rescue of activated T cells from apoptosis is dependent upon CD40/CD154 interaction.

show abstract

Physical interaction with monocytes rescues human mature CD4+ T-cell lines from anti-CD3-induced apoptosis

Cited by 2 publications

References 23 publications

Characterizing a soluble survival signal for activated lymphocytes from CD14⁺ cells

Characterizing a soluble survival signal for activated lymphocytes from CD14⁺ cells

Monocyte Rescue of Human T Cells from Apoptosis is CD40/CD154 Dependent

Contact Info

Product

Resources

About

Physical interaction with monocytes rescues human mature CD4+ T-cell lines from anti-CD3-induced apoptosis

Cited by 2 publications

References 23 publications

Characterizing a soluble survival signal for activated lymphocytes from CD14+ cells

Characterizing a soluble survival signal for activated lymphocytes from CD14+ cells

Monocyte Rescue of Human T Cells from Apoptosis is CD40/CD154 Dependent

Contact Info

Product

Resources

About

Characterizing a soluble survival signal for activated lymphocytes from CD14⁺ cells

Characterizing a soluble survival signal for activated lymphocytes from CD14⁺ cells