Physical interaction of RTBV ORFI with D1 protein of Oryza sativa and Fe/Zn homeostasis play a key role in symptoms development during rice tungro disease to facilitate the insect mediated virus transmission

Srilatha, P.; Yousuf, Faisal; Methre, Ramesh; Vishnukiran, T.; Agarwal, Shubhi; Yugandhar, Poli; Reddy, M. Raghurami; Vidyasagar, B.; Chitra, S.; Krishnaveni, D.; Triveni, S.; Brajendra,; Praveen, Shelly; Balachandran, S. M.; Subrahmanyam, D.; Mangrauthia, Satendra K.

doi:10.1016/j.virol.2018.10.012

Cited by 18 publications

(13 citation statements)

References 47 publications

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“…Rice leaf tissues were collected from healthy and tungro infected plants grown in controlled conditions at ICAR-Indian Institute of Rice Research, Hyderabad, India. To obtain tungro infected rice tissue, 15 days old plants of susceptible cultivar, Taichung Native 1 (TN1), were inoculated with the Hyderabad isolate of tungro virus using insect mediated virus transmission [38]. The insect vectors, green leafhoppers (GLH), were fed on tungro disease infected plants for 24 h (to acquire the virus particles) before releasing them onto healthy plants for 6 h. The disease symptoms were clearly observed after 15 days of virus inoculation.…”

Section: Plant Materialsmentioning

confidence: 99%

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Coordinated Action of RTBV and RTSV Proteins Suppress Host RNA Silencing Machinery

et al. 2022

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RNA silencing is as an adaptive immune response in plants that limits the accumulation or spread of invading viruses. Successful virus infection entails countering the RNA silencing machinery for efficient replication and systemic spread in the host. The viruses encode proteins with the ability to suppress or block the host silencing mechanism, resulting in severe pathogenic symptoms and diseases. Tungro is a viral disease caused by a complex of two viruses and it provides an excellent system to understand the host and virus interactions during infection. It is known that Rice tungro bacilliform virus (RTBV) is the major determinant of the disease while Rice tungro spherical virus (RTSV) accentuates the symptoms. This study brings to focus the important role of RTBV ORF-IV in disease manifestation, by acting as both the victim and silencer of the RNA silencing pathway. The ORF-IV is a weak suppressor of the S-PTGS or stable silencing, but its suppression activity is augmented in the presence of specific RTSV proteins. Among these, RTBV ORF-IV and RTSV CP3 proteins interact with each other. This interaction may lead to the suppression of localized silencing as well as the spread of silencing in the host plants. The findings present a probable mechanistic glimpse of the requirement of the two viruses in enhancing tungro disease.

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Section: Plant Materialsmentioning

confidence: 99%

“…Though RTBV is the main determinant of disease symptoms, the symptoms get accentuated in the presence of RTSV [33,36]. Stunting, yellow orange discoloration of leaves and twisting of the leaf tips are the major symptoms in plants infected with virus [37,38].…”

Section: Introductionmentioning

confidence: 99%

Coordinated Action of RTBV and RTSV Proteins Suppress Host RNA Silencing Machinery

et al. 2022

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“…Penyebaran penyakit tungro di persawahan ialah melalui wereng hijau N. virescens secara semi persisten. Srilatha et al (2019) menjelaskan bahwa RTBV berperan pada pengembangan gejala tungro pada tanaman terinfeksi, sedangkan RTSV pada penyebaran penyakit dari satu tanaman ke tanaman lain. Gejala utama penyakit tungro ialah kerdil dan klorosis.…”

Section: Pembahasanunclassified

Resistance of Several Local Rice Varieties to Isolate Tungro Virus from Muara

Ruimassa¹,

Manzila

Temaja

et al. 2022

J Fitopatol Indones

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Penyakit tungro merupakan salah satu penyakit penting pada tanaman padi karena menimbulkan gejala tanaman kerdil dan mampu menyebabkan kehilangan hasil yang nyata. Penanaman varietas tahan merupakan strategi pengendalian yang direkomendasikan untuk mengatasi penyakit tungro. Penelitian dilakukan untuk mencari sumber ketahanan genetik dari varietas padi lokal yang dapat digunakan dalam perakitan varietas tahan penyakit tungro. Percobaan disusun dalam rancangan penelitian deskriptif dengan teknik observasi. Observasi dilakukan terhadap 16 varietas padi lokal dengan setiap varietas terdiri atas 30 tanaman contoh sehingga jumlah keseluruhan tanaman yang digunakan sebanyak 480 tanaman. Isolat virus tungro dari pertanaman padi di Muara, Bogor diinokulasikan ke tanaman uji melalui serangga vektor wereng hijau (Nephotettix virescens). Hasil penelitian menunjukkan bahwa ketahanan varietas lokal bervariasi berdasarkan nilai indeks penyakit, insidensi penyakit dan penyusutan tinggi tanaman. Varietas TN1 merupakan varietas pembanding dengan reaksi rentan, sedangkan varietas padi lokal dibedakan atas tahan (‘Utri Merah’), moderat (‘Karau’, ‘Bekongan’, ‘Bujang Berinai’, ‘Srogel Abang’, ‘Pulut Garu’, ‘Randu Kisaran’, ‘Siredep’, ‘Katimpung’, ‘Pulut Sawijan’, ‘Dube’), moderat cenderung rentan (‘Blumbungan’, ‘Tjere bandung’, ‘Sempor’) dan rentan (‘Rumbai’). Semua varietas dapat terinfeksi (insidensi penyakit 27–76%), tetapi indeks keparahan penyakit sangat bervariasi (3–7). Semakin berat keparahan penyakit menyebabkan penyusutan tinggi tanaman semakin tinggi. ‘Srogel Abang’ merupakan varietas yang bermanfaat untuk perakitan varietas padi unggul tahan penyakit tungro karena bersifat moderat dengan persentase penyusutan tinggi tanaman yang rendah (36%) sehingga produktivitas tanaman masih dapat dipertahankan.

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“…Rice leaf tissues were collected from healthy and tungro infected plants grown in controlled conditions at ICAR-IIRR, Hyderabad. To obtain tungro infected rice tissue, 15 days old plants of susceptible cultivar, Taichung Native 1 (TN1) were inoculated with Hyderabad isolate of tungro virus using insect mediated virus transmission (Srilatha et al, 2019). The insect vectors, green leafhoppers (GLH), were fed on tungro disease infected plants for 24h (to acquire the virus particles) before releasing them onto healthy plants for 6h.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Though RTBV is the main determinant of disease symptoms, the presence of RTSV accentuates the symptoms (Hull, 1996;Borah et al, 2013). Stunting, yellow orange discoloration of leaves and twisting of leaf tips are the major symptoms in plants infected with Tungro virus (Hibino et al, 1978;Dasgupta et al, 1991;Srilatha et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Coordinated action of RTBV and RTSV proteins suppress host RNA silencing machinery

Anand

Pinninti

Tripathi

et al. 2021

Preprint

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RNA silencing is as an adaptive immune response in plants that limits accumulation or spread of invading viruses. Successful virus infection entails countering the RNA silencing for efficient replication and systemic spread in the host. The viruses encode proteins having the ability to suppress or block the host silencing mechanism, resulting in severe pathogenic symptoms and diseases. Tungro virus disease caused by a complex of two viruses provides an excellent system to understand these host and virus interactions during infection. It is known that Rice tungro bacilliform virus (RTBV) is the major determinant of the disease while Rice tungro spherical virus (RTSV) accentuates the symptoms. This study brings to focus the important role of RTBV ORF-IV in Tungro disease manifestation, by acting as both the victim and silencer of the RNA silencing pathway. The ORF-IV is a weak suppressor of the S-PTGS or pre-established stable silencing but its suppression activity is augmented in the presence of RTSV proteins. The RTBV and RTSV proteins interact to suppress localized silencing as well as spread of silencing, in the host plants.

show abstract

Physical interaction of RTBV ORFI with D1 protein of Oryza sativa and Fe/Zn homeostasis play a key role in symptoms development during rice tungro disease to facilitate the insect mediated virus transmission

Cited by 18 publications

References 47 publications

Coordinated Action of RTBV and RTSV Proteins Suppress Host RNA Silencing Machinery

Coordinated Action of RTBV and RTSV Proteins Suppress Host RNA Silencing Machinery

Resistance of Several Local Rice Varieties to Isolate Tungro Virus from Muara

Coordinated action of RTBV and RTSV proteins suppress host RNA silencing machinery

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