Physical exercise prevents behavioral alterations in a reserpine-treated zebrafish: A putative depression model

Martins, Gabriela Madalena de Melo; Pettersen, Bárbara; Rübensam, Gabriel; Silva, Júlia Maria Kuhl da; Gaspary, Karina Vidarte; Wiprich, Melissa Talita; Altenhofen, Stefani; Bonan, Carla Denise

doi:10.1016/j.pbb.2022.173455

Cited by 8 publications

(7 citation statements)

References 40 publications

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“…All STMs are available via data repository (Section 0). We observed, but did not quantify, a reduction in reserpine‐treated larvae swimming behavior that was consistent with other reports of zebrafish hypoactivity 47–54 …”

Section: Resultssupporting

confidence: 90%

“…These findings suggest the gut may have been tonically contracted, which may explain for the observed decreases in contraction events. Increased skeletal muscle rigidity is a hallmark feature in reserpinized murine models, 61 but reserpine‐induced rigidity of smooth and/or skeletal muscles has not been directly explored in studies with reserpine‐treated zebrafish 47–54,60 . We did not investigate further, so we cannot conclude with certainty that the gut was tonically contracted or speak to the underlying molecular mechanisms.…”

Section: Discussionmentioning

confidence: 97%

“…Increased skeletal muscle rigidity is a hallmark feature in reserpinized murine models, 61 but reserpineinduced rigidity of smooth and/or skeletal muscles has not been directly explored in studies with reserpine-treated zebrafish. [47][48][49][50][51][52][53][54]60 We did not investigate further, so we cannot conclude with certainty that the gut was tonically contracted or speak to the underlying molecular mechanisms. Another potential explanation that we cannot rule out is that the reduction in the dynamic range of the fluorescence signal could have impeded detection of periodic fluctuations caused by propagating contraction events.…”

Section: Reserpine Treatment Suggests Involvement Of Monoamines In Ea...mentioning

confidence: 99%

“…We observed, but did not quantify, a reduction in reserpine-treated larvae swimming behavior that was consistent with other reports of zebrafish hypoactivity. [47][48][49][50][51][52][53][54]…”

Section: Zebrafish Larvae Treated With Reserpine Exhibit Reduced Gut ...mentioning

confidence: 99%

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Systematic analysis of proximal midgut‐ and anorectal‐originating contractions in larval zebrafish using event feature detection and supervised machine learning algorithms

Cassidy,

Flores,

Trinh Nguyen

et al. 2023

Neurogastroenterology Motil

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BackgroundZebrafish larvae are translucent, allowing in vivo analysis of gut development and physiology, including gut motility. While recent progress has been made in measuring gut motility in larvae, challenges remain which can influence results, such as how data are interpreted, opportunities for technical user error, and inconsistencies in methods.MethodsTo overcome these challenges, we noninvasively introduced Nile Red fluorescent dye to fill the intraluminal gut space in zebrafish larvae and collected serial confocal microscopic images of gut fluorescence. We automated the detection of fluorescent‐contrasted contraction events against the median‐subtracted signal and compared it to manually annotated gut contraction events across anatomically defined gut regions. Supervised machine learning (multiple logistic regression) was then used to discriminate between true contraction events and noise. To demonstrate, we analyzed motility in larvae under control and reserpine‐treated conditions. We also used automated event detection analysis to compare unfed and fed larvae.Key ResultsAutomated analysis retained event features for proximal midgut‐originating retrograde and anterograde contractions and anorectal‐originating retrograde contractions. While manual annotation showed reserpine disrupted gut motility, machine learning only achieved equivalent contraction discrimination in controls and failed to accurately identify contractions after reserpine due to insufficient intraluminal fluorescence. Automated analysis also showed feeding had no effect on the frequency of anorectal‐originating contractions.Conclusions & InferencesAutomated event detection analysis rapidly and accurately annotated contraction events, including the previously neglected phenomenon of anorectal contractions. However, challenges remain to discriminate contraction events based on intraluminal fluorescence under treatment conditions that disrupt functional motility.

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Section: Resultssupporting

confidence: 90%

Section: Discussionmentioning

confidence: 97%

Section: Reserpine Treatment Suggests Involvement Of Monoamines In Ea...mentioning

confidence: 99%

Section: Zebrafish Larvae Treated With Reserpine Exhibit Reduced Gut ...mentioning

confidence: 99%

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Systematic analysis of proximal midgut‐ and anorectal‐originating contractions in larval zebrafish using event feature detection and supervised machine learning algorithms

Cassidy,

Flores,

Trinh Nguyen

et al. 2023

Neurogastroenterology Motil

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“…Indeed, we measured only transitions from different compartments (vertical movements from bottom to top in the Novel Tank Diving test or horizontal movements towards the empty or enriched compartment in the Compartment Preference test) and not general (not motivated) vertical or horizontal movements. In any case, we did not observe a reduced motor activity after reserpine exposure at the difference of de Melo Martins et al (2022) who found a reduced swimming activity. The different ages of zebrafish used in our study (6 vs 12 months) and the different exposure methods (two animals at a time vs all together) could account for the conflicting data.…”

Section: Discussioncontrasting

confidence: 69%

Fluoxetine rescues the depressive-like behaviour induced by reserpine and the altered emotional behaviour induced by nicotine withdrawal in zebrafish: Involvement of tyrosine hydroxylase

Braida,

Ponzoni,

Dellarole

et al. 2023

J Psychopharmacol

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Background: Nicotine cessation leads to anxiety and depression. Aims: The suitability of the zebrafish model of anhedonia using reserpine and fluoxetine was evaluated. Fluoxetine was also used to reduce nicotine withdrawal-induced anhedonic state. Methods: Zebrafish were exposed to reserpine (40 mg/l) and then to fluoxetine (0.1 mg/l) for 1 week. Anhedonia was evaluated in the Novel Tank Diving and Compartment Preference tests. Another group was exposed to nicotine (1 mg/l/2 weeks) and then exposed to fluoxetine. Anxiety and anhedonia were evaluated 2–60 days after. Tyrosine hydroxylase (TH) immunoreactivity and microglial morphology (labelled by 4C4 monoclonal antibody) in the parvocellular pretectal nucleus (PPN), dorsal part, and of calcitonin gene-related peptide (CGRP) in the hypothalamus were also analysed. Results: Less time in the top and increased latency to the top in reserpine compared to a drug-free group was found. Fluoxetine rescued reserpine-induced the reduced time in the top. Seven and 30 days after nicotine withdrawal more time in the bottom and similar time in the Compartment Preference test, rescued by fluoxetine, were shown. In the PPN, 30-day withdrawal induced an increase in TH immunoreactivity, but fluoxetine induced a further significant increase. No changes in PPN microglia morphology and hypothalamic CGRP were detected. Conclusions: Our findings validate the suitability of the zebrafish model of anhedonia using the reserpine-induced depression-like behaviour and the predictivity using fluoxetine. Fluoxetine rescued nicotine withdrawal-induced anhedonic state, opening the possibility to screen new drugs to alleviate anxiety and depression in smokers during abstinence.

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Noscapine modulates hypoxia‐induced angiogenesis and hemodynamics: Insights from a zebrafish model investigation

Nathan,

Shameera,

Sivakumar

et al. 2024

Drug Development Research

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We investigated the angiogenesis‐modulating ability of noscapine in vitro using osteosarcoma cell line (MG‐63) and in vivo using a zebrafish model. MTT assay and the scratch wound healing assay were performed on the osteosarcoma cell line (MG‐63) to analyze the cytotoxic effect and antimigrative ability of noscapine, respectively. We also observed the antiangiogenic ability of noscapine on zebrafish embryos by analyzing the blood vessels namely the dorsal aorta, and intersegmental vessels development at 24, 48, and 72 h postfertilization. Real‐time polymerase chain reaction was used to analyze the hypoxia signaling molecules' gene expression in MG‐63 cells and zebrafish embryos. The findings from the scratch wound healing demonstrated that noscapine stopped MG‐63 cancer cells from migrating under both hypoxia and normoxia. Blood vessel development and the heart rate in zebrafish embryos were significantly reduced by noscapine under both hypoxia and normoxia which showed the hemodynamics impact of noscapine. Noscapine also downregulated the cobalt chloride (CoCl2) induced hypoxic signaling molecules' gene expression in MG‐63 cells and zebrafish embryos. Therefore, noscapine may prevent MG‐63 cancer cells from proliferating and migrating, as well as decrease the formation of new vessels and the production of growth factors linked to angiogenesis in vivo under both normoxic and hypoxic conditions.

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Physical exercise prevents behavioral alterations in a reserpine-treated zebrafish: A putative depression model

Cited by 8 publications

References 40 publications

Systematic analysis of proximal midgut‐ and anorectal‐originating contractions in larval zebrafish using event feature detection and supervised machine learning algorithms

Systematic analysis of proximal midgut‐ and anorectal‐originating contractions in larval zebrafish using event feature detection and supervised machine learning algorithms

Fluoxetine rescues the depressive-like behaviour induced by reserpine and the altered emotional behaviour induced by nicotine withdrawal in zebrafish: Involvement of tyrosine hydroxylase

Noscapine modulates hypoxia‐induced angiogenesis and hemodynamics: Insights from a zebrafish model investigation

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