Physical discrimination between human T‐lymphocyte subpopulations by means of light scattering, revealing two populations of T8‐positive cells

Terstappen, Leonardus Wendelinus Mathias Marie; Grooth, B.G. de; Nolten, G.M.J.; Napel, C.H.H. ten; Berkel, Willem J.H. van; Greve, Jan

doi:10.1002/cyto.990070209

Cited by 36 publications

(23 citation statements)

References 19 publications

(14 reference statements)

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“…It was demonstrated that these subpopulations consist of regulatory and Blymphocytes (low orthogonal light scattering signal) and cytotoxic lymphocytes (high orthogonal light scattering signal). In another report we have shown that the CD8 + lymphocyte population could be divided in a suppressor-and a cytotoxic lymphocyte CD8 + subpopulation [17].…”

Section: Introductionmentioning

confidence: 85%

“…A large variation in the occurrence of CD8 + subpopulations of healthy donors is observed [17]. CD8b+/CD8 + ratios varied from 17 to 69 (mean 39).…”

Section: Cd8a and Cd8b Determinationmentioning

confidence: 99%

“…Lymphocytes were obtained after density separation as described in detail elsewhere [17]. Lymphocytes were adjusted to a concentration of 1.107 lymphocytes per ml with a standard buffer (PBS containing 0.05% sodium azide and 1% bovine serum albumin (BSA)).…”

Section: Preparation Of Lymphocytesmentioning

confidence: 99%

“…The FCM was described in detail elsewhere [17]. A 3-W argon ion laser tuned at 488 nm operating at a light intensity of 100mW was used.…”

Section: Flow Cytometry Measurementsmentioning

confidence: 99%

“…Determination of CD8 + subpopulations was performed by dividing the orthogonal light scatter histograms into two components with a low (CD8a) and a large (CD8b) orthogonal light scattering signal as described in detail in earlier reports [16,17]. In B-CLL patients the occurrence of both populations strongly differs from that of healthy donors as is shown in Fig.…”

Section: Cd8a and Cd8b Determinationmentioning

confidence: 99%

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Abnormal distribution of CD8 subpopulation in B-chronic lymphocytic leukemia identified by flow cytometry

Terstappen

Grooth

Berkel³

et al. 1988

Leukemia Research

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Abstract--We studied the occurrence of T-cell subpopulations for patients with B-cell chronic lymphocytic leukemia. The CD8 ÷ population was divided into CD8 ÷ suppressor (CD8a ÷) and CD8 ÷ cytotoxic (CD8b ÷) lymphocytes using difference in orthogonal light scattering.Average CD4÷/CD8+ratios determined for all patients were decreased. For individual patients this sometimes was not true. In contrast CD4÷/CD8a + ratios were markedly increased in all individual patients. The CD8 ÷ lymphocytes appeared to consist mainly of CD8b+lymphocytes. Moreover the CD8b+/CD8 ÷ ratio correlated with clinical stage: untreated patients (stage 0 of Rai) have smaller CD8b+/CD8 + ratios than patients with advanced stages of Rai.

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Section: Introductionmentioning

confidence: 85%

“…A large variation in the occurrence of CD8 + subpopulations of healthy donors is observed [17]. CD8b+/CD8 + ratios varied from 17 to 69 (mean 39).…”

Section: Cd8a and Cd8b Determinationmentioning

confidence: 99%

Section: Preparation Of Lymphocytesmentioning

confidence: 99%

“…The FCM was described in detail elsewhere [17]. A 3-W argon ion laser tuned at 488 nm operating at a light intensity of 100mW was used.…”

Section: Flow Cytometry Measurementsmentioning

confidence: 99%

Section: Cd8a and Cd8b Determinationmentioning

confidence: 99%

See 3 more Smart Citations

Abnormal distribution of CD8 subpopulation in B-chronic lymphocytic leukemia identified by flow cytometry

Terstappen

Grooth

Berkel³

et al. 1988

Leukemia Research

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Increase in okm1 + granular lymphocytes in patients with rheumatoid arthritis

Cooper¹,

Roessner²,

Ferriss³

et al. 1987

Arthritis & Rheumatism

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Patients with very active rheumatoid arthritis that was being treated only with nonsteroidal antiinflammatory drugs had increased numbers of peripheral blood OKMl+ lymphocytes. In 3 patients, 90" light scatter analysis revealed a double lymphocyte peak. When sorted, the high scatter peak contained a large percentage of granular lymphocytes. Patients with mildto-moderately active rheumatoid arthritis had normal levels of OKM1+ lymphocytes, but when the drugs were discontinued, the activity of the disease and the numbers of OKMl+ cells increased. Administration of piroxicam was associated with clinical improvement and a decrease in levels of OKM1+ cells. OKM1+ granular lymphocytes are increased in some rheumatoid arthritis patients, and their numbers may correlate with clinical disease activity and/or therapy.Rheumatoid arthritis (RA) is characterized as an autoimmune disorder, with components of both cellular and humoral immune dysfunction (1,2). The

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Light‐scattering polarization measurements as a new parameter in flow cytometry

et al. 1987

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Polarization measurement of orthogonal light scattering is introduced as a new optical parameter in flow cytometry.In the experimental setup, the electrical field of the incident laser beam is polarized in the direction of the sample flow. The intensity of the orthogonal light scattering polarized along the direction of the incoming laser beam is called depolarized orthogonal light scattering. Theoretical analysis shows that for small values of the detection aperture, the measured depolarization is caused by anisotropic cell structures and multiple scattering processes inside the cell.Measurements of the orthogonal depolarized light scattering in combination with the normal orthogonal light scattering of human leucocytes revealed two populations of granulocytes. By means of cell sorting it was shown that the granulocytes with a relatively high depolarization are eosinophilic granulocytes. Similar experiments with human lymphocytes revealed a minor subpopulation of yet-unidentified lymphocytes with a relative large orthogonal light-scattering depolarization. The results were obtained with an argon ion laser tuned at different wavelengths as well as with a 630-nm helium neon laser.These results show that measurement of depolarized orthogonal light scattering is a useful new parameter for flow-cytometric cell differentiation.

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Physical discrimination between human T‐lymphocyte subpopulations by means of light scattering, revealing two populations of T8‐positive cells

Cited by 36 publications

References 19 publications

Abnormal distribution of CD8 subpopulation in B-chronic lymphocytic leukemia identified by flow cytometry

Abnormal distribution of CD8 subpopulation in B-chronic lymphocytic leukemia identified by flow cytometry

Increase in okm1 + granular lymphocytes in patients with rheumatoid arthritis

Light‐scattering polarization measurements as a new parameter in flow cytometry

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