Physical Cytometry: Detecting Mass-Related Properties of Single Cells

Zhao, Yuliang; Gu, Long; Sun, Hui; Sha, Xiaopeng; Li, Wen J.

doi:10.1021/acssensors.1c01787

Cited by 5 publications

(3 citation statements)

References 96 publications

(153 reference statements)

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“…These methods are versatile, not limited by cell type, and can be complemented with other imaging techniques, although they are not the primary focus of this review. For more comprehensive information on detecting single‐cell mass, including techniques such as single‐cell mass cytometry, suspended image cytometry, suspended microchannel resonator, phase‐shift interferometry, and optoelectric cell manipulation, readers are encouraged to refer to recent reviews by other research groups [133].…”

Section: Single‐cell Biophysical Property Detection Techniquesmentioning

confidence: 99%

Microfluidic characterization of single‐cell biophysical properties and the applications in cancer diagnosis

Li,

Xue,

et al. 2023

Electrophoresis

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Single‐cell biophysical properties play a crucial role in regulating cellular physiological states and functions, demonstrating significant potential in the fields of life sciences and clinical diagnostics. Therefore, over the last few decades, researchers have developed various detection tools to explore the relationship between the biophysical changes of biological cells and human diseases. With the rapid advancement of modern microfabrication technology, microfluidic devices have quickly emerged as a promising platform for single‐cell analysis offering advantages including high‐throughput, exceptional precision, and ease of manipulation. Consequently, this paper provides an overview of the recent advances in microfluidic analysis and detection systems for single‐cell biophysical properties and their applications in the field of cancer. The working principles and latest research progress of single‐cell biophysical property detection are first analyzed, highlighting the significance of electrical and mechanical properties. The development of data acquisition and processing methods for real‐time, high‐throughput, and practical applications are then discussed. Furthermore, the differences in biophysical properties between tumor and normal cells are outlined, illustrating the potential for utilizing single‐cell biophysical properties for tumor cell identification, classification, and drug response assessment. Lastly, we summarize the limitations of existing microfluidic analysis and detection systems in single‐cell biophysical properties, while also pointing out the prospects and future directions of their applications in cancer diagnosis and treatment.

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Section: Single‐cell Biophysical Property Detection Techniquesmentioning

confidence: 99%

Microfluidic characterization of single‐cell biophysical properties and the applications in cancer diagnosis

Li,

Xue,

et al. 2023

Electrophoresis

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“…M ICROELECTROMECHANICAL systems (MEMSs) have attracted great attention in the field of physical and chemical sensors [1], [2]. In addition, suspended microchannel resonators (SMRs) have been developed for sensing biomaterials because their resonance frequency is highly sensitive, thereby enabling the detection of nano/microscale materials [3], [4], [5].…”

Section: Introductionmentioning

confidence: 99%

Silicon-Based Suspended Microchannel Resonator Developed Using Au Thermal Diffusion Bonding for Mass Sensing of Biomaterials

Funayama,

Miura,

Arai

et al. 2024

J. Microelectromech. Syst.

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Suspended microchannels are of great interest in applications such as physical and chemical sensor systems. In this study, we developed a suspended microchannel resonator (SMR) by bonding two separate Au-coated silicon-insulatorsilicon substrates via thermal diffusion bonding. To obtain a secure bond between Au films, we investigated different bonding temperatures and Au film thicknesses. As a result, we successfully fabricated an SMR. We show that the developed resonator has a resonance frequency of 229.55 kHz and a quality factor of 171 for the empty channel. The response of the channel to absolute mass was 18.7 pg/Hz. The measurement results were in good agreement with the results of numerical simulations. In addition, we estimated the practical mass detectability of the developed SMR via statistical analysis. The developed SMR enabled mass detection with a resolution of 710.6 pg. Our SMR can be produced via typical semiconductor fabrication technology, which is advantageous in terms of mass production.[

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“…The processes of cell differentiation, adhesion, and migration are regulated by the mechanical properties of cells, which are closely related to cell type, state, and function. , The assay of single-cell mechanical properties has received increasing attention due to cellular heterogeneity. Studies have shown that single-cell mechanical properties can provide crucial insights into cell isolation, disease diagnosis, immunological analysis, etc. , Therefore, it is necessary to study the effects of cryopreservation on cell mechanical properties from the single-cell perspective, which will in-depth comprehend the mechanism of cellular cryoinjury.…”

Section: Introductionmentioning

confidence: 99%

Label-Free and Noninvasive Single-Cell Characterization for the Viscoelastic Properties of Cryopreserved Human Red Blood Cells Using a Dielectrophoresis-On-a-Chip Approach

Wang

Shen

et al. 2022

Anal. Chem.

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Successful human red blood cell cryopreservation techniques have been gradually developed in recent decades, with great potential for use in clinical medicine and basic research. The mechanical properties of a single cell are important clues to reveal the physiological and pathological state of the red blood cell, but they have not been used to assess the physiological state of the cell after cryopreservation. Herein, we investigated the effects of cryopreservation processes on human red blood cell biomechanics by means of a microfluidic, label-free, synchronous, and nondestructive electrodeformation assay. We found that the effects of viscoelasticity of the red blood cell membrane between permeable and impermeable cryoprotectants were different. Our findings showed that high freeze−thaw recovery did not mean that the recovered cells had excellent viscoelasticity. The results demonstrate that single-cell viscoelasticity is an irreplaceable indicator for assessing the quality of the recovered cells and help us to deepen understanding of the cryoinjury mechanism. Assessment of the single cell viscoelasticity offers significant potential for application in optimizing the cryopreservation process and screening optimal cryoprotectants.

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Physical Cytometry: Detecting Mass-Related Properties of Single Cells

Cited by 5 publications

References 96 publications

Microfluidic characterization of single‐cell biophysical properties and the applications in cancer diagnosis

Microfluidic characterization of single‐cell biophysical properties and the applications in cancer diagnosis

Silicon-Based Suspended Microchannel Resonator Developed Using Au Thermal Diffusion Bonding for Mass Sensing of Biomaterials

Label-Free and Noninvasive Single-Cell Characterization for the Viscoelastic Properties of Cryopreserved Human Red Blood Cells Using a Dielectrophoresis-On-a-Chip Approach

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