Physical-chemical properties of C-phycocyanin isolated from an acido-thermophilic eukaryote, <i>Cyanidium caldarium</i>

Kao, Oranda H. W.; Edwards, Mercedes R.; Berns, Donald S.

doi:10.1042/bj1470063

Cited by 42 publications

(22 citation statements)

References 38 publications

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“…was found to degrade more than 80 % at higher temperatures ranging from 26 to 74°C (Chaiklahan et al 2012). Structurally, the conformation of PC has been reported to change into 23 % hexameric form at pH 5.5-6.0, while it persists up to 82 % in trimeric form at pH 7.0 (Kao et al 1975;Adams et al 1979;Chaiklahan et al 2012). This indicates that the trimeric form of PC is more stable at neutral pH which is mostly preferred for extraction of PBPs.…”

Section: Discussionmentioning

confidence: 89%

Thermokinetic stability of phycocyanin and phycoerythrin in food-grade preservatives

Kannaujiya

Sinha

2015

J Appl Phycol

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Phycocyanin (PC) and phycoerythrin (PE) are the main phycobiliproteins (PBPs) with application as colorants in food industries. In the present study, the thermokinetic stability of PBPs extracted and purified from a hot spring cyanobacterium, Nostoc sp. strain HKAR-2, was investigated. The individual components of PC and PE were isolated with a high purity ratio of 3.18 (A 615 /A 280 ) and 7.2 (A 563 /A 280 ), respectively. The thermokinetic stability of purified PC and PE was studied in the presence of edible preservatives such as benzoic acid, citric acid, sucrose, ascorbic acid, and calcium chloride over 30 days of incubation at 4, 25, and 40°C. The rate of degradation (k value) of PC/PE was increased in the control (without preservative), while it declined in the presence of preservatives. The k value (day −1 ) was found to be the lowest for benzoic acid-treated PC/PE in comparison to the control at 4°C. The rate of degradation also declined in benzoic acid-treated PC (0.008 day −1 ) and PE (0.012 day −1 ) at 40°C in comparison with the control. Citric acid and sucrose were also found to maintain the stability of both PC and PE at the same temperature. Calcium chloride and ascorbic acid were shown to be the preservatives that support the least stability of PC and PE in comparison to the other preservatives studied. Overall, benzoic acid was found to be the best preservative for both PC and PE at 4°C.

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Section: Discussionmentioning

confidence: 89%

Thermokinetic stability of phycocyanin and phycoerythrin in food-grade preservatives

Kannaujiya

Sinha

2015

J Appl Phycol

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“…Kao et al (1975) have demonstrated that this C-phycocyanin is not colddissociated. Its properties remain unchanged from 10 to 50°C (Eisele et al 2000), and it can be considered a temperature-resistant protein.…”

Section: Cyanidium Caldariummentioning

confidence: 96%

Biliproteins and phycobilisomes from cyanobacteria and red algae at the extremes of habitat

Samsonoff

MacColl

2001

Archives of Microbiology

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This review considers the properties of biliproteins from cyanobacteria and red algae that grow in extreme habitats. Three situations are presented: cyanobacteria that grow at high temperatures; a red alga that grows in acidic conditions at high temperature; and an Antarctic red alga that grows in the cold in dim light conditions. In particular, the properties of their biliproteins are compared to those from organisms from more usual environments. C-phycocyanins from two cyanobacteria able to grow at high temperatures are found to differ in their stabilities when compared to C-phycocyanin from mesophilic algae. They differ in opposite ways, however. One is more stable to dissociation than the mesophilic protein, and the other is more easily dissociated at low temperatures. The thermophilic proteins resist thermal denaturation much better than the mesophilic proteins. The most thermophilic cyanobacterium has a C-phycocyanin with a unique blue-shifted absorption maximum which does not appear to be part of the adaptation of the cyanobacterium to high temperature. The C-phycocyanin from the high-temperature red alga is able to resist dissociation better than mesophilic C-phycocyanins. Electron micrographs show the phycobilisomes of these algae. The Antarctic alga grows under ice at some distance down the water column. Its R-phycoerythrin has a novel absorption spectrum that gives the alga an improved ability to harvest blue light. This may enhance its survival in its light-deprived habitat.

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“…Freeze thawing in distilled water (Padgett and Krogmann 1987) 100 mM phosphate buffer, 200 mM NaCl,10 mM EDTA, 0.02% Triton X-100 & 1 mg/ml lysozyme (Vernet et al 1990) DEAE -Cellulose (Bennett and Bogarad 1971) Using mechanical tissue homogenizers (Kao et al 1975) 1 M Tris (pH 8.0), 0.5 M EDTA, 20% sucrose (w/v) with 5 mg/ml lysozyme (Bhaskar et al 2005) DEAE-sepharose CL-6B (Zhang and Chen 1999) Extraction of dried bio mass at 4°C (Doker 2005) 1% rivanol (Minkova et al 2003) Streamline -DEAE (Bremjo 2006) Q-Sepharose (Sajilata and Singhal 2006) products to be manufactured as well as improvements in bioprocess engineering.…”

Section: Resultsmentioning

confidence: 99%

Phycobiliproteins as a commodity: trends in applied research, patents and commercialization

2007

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Phycobiliproteins are a group of colored proteins commonly present in cyanobacteria and red algae possessing a spectrum of applications. They are extensively commercialized for fluorescent applications in clinical and immunological analysis. They are also used as a colorant, and their therapeutic value has also been categorically demonstrated. However, a comprehensive knowledge and technological base for augmenting their commercial utilities is lacking. Hence, this work is focused towards this objective by means of analyzing global patents and commercial activities with application oriented research. Strategic mining of patents was performed from global patent databases resulting in the identification of 297 patents on phycobiliproteins. The majority of the patents are from USA, Japan and Europe. Patents are grouped into fluorescent applications, general applications and production aspects of phycobiliproteins and the features of each group are discussed. Commercial and applied research activities are compared in parallel. It revealed that US patents are mostly related to fluorescent applications while Japanese are on the production, purification and application for therapeutic and diagnostic purposes. Fluorescent applications are well represented in research, patents and commercial sectors. Biomedical properties documented in research and patents are not ventured commercially. Several novel applications are reported only in patents. The paper further pinpoints the plethora of techniques used for cell breakage and for extraction and purification of phycobiliproteins. The analysis identifies the lacuna and suggests means for improvements in the application and production of phycobiliproteins.

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Physical-chemical properties of C-phycocyanin isolated from an acido-thermophilic eukaryote, Cyanidium caldarium

Cited by 42 publications

References 38 publications

Thermokinetic stability of phycocyanin and phycoerythrin in food-grade preservatives

Thermokinetic stability of phycocyanin and phycoerythrin in food-grade preservatives

Biliproteins and phycobilisomes from cyanobacteria and red algae at the extremes of habitat

Phycobiliproteins as a commodity: trends in applied research, patents and commercialization

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