Physical characterization of polycaprolactone scaffolds

Estellés, Jorge Más; Vidaurre, Ana; Dueñas, José María Meseguer; Cortázar, Isabel Castilla

doi:10.1007/s10856-006-0101-2

Cited by 87 publications

(68 citation statements)

References 32 publications

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“…Results obtained for the Young's modulus corresponding to the third scan (E 3 ) followed a similar trend to that of the first one, although with higher values. As has been said in previous papers [33], moduli for the second and third scans are higher than that for the first scan, due to pore collapse and the densification effect. This effect is related to the increase in the deformation measured between the first and third scans.…”

Section: Mechanical Testingsupporting

confidence: 60%

Hydrolytic and enzymatic degradation of a poly(ε-caprolactone) network

Castilla-Cortázar¹,

Más-Estellés²,

Dueñas³

et al. 2012

Polymer Degradation and Stability

144

116

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Long-term hydrolytic and enzymatic degradation profiles of poly(ε-caprolactone) (PCL) networks were obtained. The hydrolytic degradation studies were performed in water and phosphate buffer solution (PBS) for 65 weeks. In this case, the degradation rate of PCL networks was faster than previous results in the literature on linear PCL, reaching a weight loss of around 20% in 60 weeks after immersing the samples either in water or PBS conditions. The enzymatic degradation rate in Pseudomonas Lipase for 14 weeks was also studied, with the conclusion being that the degradation profile of PCL networks is lower than for linear PCL, also reaching a 20% weight loss. The weight lost, degree of swelling, and calorimetric and mechanical properties as a function of degradation time were obtained. Furthermore, the morphological changes in the samples were studied carefully through electron microscopy and crystal size through X-ray diffraction. The changes in some properties over the degradation period such as crystallinity, crystal size and Young's modulus were smaller in the case of enzymatic studies, highlighting differences in the degradation mechanism in the two studies, hydrolytic and enzymatic.

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Section: Mechanical Testingsupporting

confidence: 60%

Hydrolytic and enzymatic degradation of a poly(ε-caprolactone) network

Castilla-Cortázar¹,

Más-Estellés²,

Dueñas³

et al. 2012

Polymer Degradation and Stability

144

116

View full text Add to dashboard Cite

show abstract

“…The grooves were embossed into the biodegradable polymer polycaprolactone (PCL, an FDA-approved biomaterial with good biocompatibility and excellent micro-and nanoreplication characteristics [17,18]). In order to facilitate improved cell growth to obtain higher protein yields, a flow system bioreactor was used to perfuse the basal medium [19].…”

Section: Introductionmentioning

confidence: 99%

Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance

et al. 2009

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“…The latter is more susceptible to hydrolytic degradation due to its ester bonds [-C-O-(C O)-]. 50 Interestingly, the introduction of HAP into the scaffold did not modify the degradation rate. Many authors have studied the degradation of polymeric materials under different conditions.…”

Section: Fernández Et Almentioning

confidence: 99%

Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility

Fernández¹,

Cortizo²,

Cortizo³

2014

j biomater tissue eng

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The present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly--caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco's modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL + HAP or Blend + HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer-and HAP-based scaffolds could potentially be used as bone substitute materials.

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Physical characterization of polycaprolactone scaffolds

Cited by 87 publications

References 32 publications

Hydrolytic and enzymatic degradation of a poly(ε-caprolactone) network

Hydrolytic and enzymatic degradation of a poly(ε-caprolactone) network

Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance

Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility

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