Physical Basis for Membrane-Charge Selectivity of Cationic Antimicrobial Peptides

Taheri-Araghi, Sattar; Ha, Bae‐Yeun

doi:10.1103/physrevlett.98.168101

Cited by 27 publications

(34 citation statements)

References 15 publications

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“…suis LTA D-alanylation contributes to antimicrobial peptide resistance and decreases susceptibility to neutrophil killing. CAMPs kill bacteria by forming pores in the cytoplasmic membrane (30). Introduction of positively charged D-alanine residues into the LTA would reduce the global negative charge of the S. suis envelope, thus providing the bacterium with a physical mechanism for resistance to the action of CAMPs (23).…”

Section: Resultsmentioning

confidence: 99%

d -Alanylation of Lipoteichoic Acid Contributes to the Virulence of Streptococcus suis

et al. 2008

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We generated by allelic replacement a ⌬dltA mutant of a virulent Streptococcus suis serotype 2 field strain and evaluated the contribution of lipoteichoic acid (LTA) D-alanylation to the virulence traits of this swine pathogen and zoonotic agent. The absence of LTA D-alanylation resulted in increased susceptibility to the action of cationic antimicrobial peptides. In addition, and in contrast to the wild-type strain, the ⌬dltA mutant was efficiently killed by porcine neutrophils and showed diminished adherence to and invasion of porcine brain microvascular endothelial cells. Finally, the ⌬dltA mutant was attenuated in both the CD1 mouse and porcine models of infection, probably reflecting a decreased ability to escape immune clearance mechanisms and an impaired capacity to move across host barriers. The results of this study suggest that LTA D-alanylation is an important factor in S. suis virulence.

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Section: Resultsmentioning

confidence: 99%

d -Alanylation of Lipoteichoic Acid Contributes to the Virulence of Streptococcus suis

et al. 2008

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“…Recent studies have attempted to develop a theoretical and experimental understanding of this chemical equilibrium in the context of antimicrobial peptides using simplified parameters such as hydrophobicity and charge (Lad et al, 2007;Taheri-Araghi and Ha, 2007). Among all the peptides examined in this study, the Tat peptide was the least hydrophobic and least likely to insert into the bilayers.…”

Section: At Low Peptide Concentration All Cationic Peptides Reduced mentioning

confidence: 95%

Cationic peptide-induced remodelling of model membranes: Direct visualization by in situ atomic force microscopy

Shaw

Epand

Hsu

et al. 2008

Journal of Structural Biology

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“…Taking into account the zwitterionic nature of the lipids is the main objective of the present work. Previous studies on various aspects concerning the adsorption of macroions onto a mixed membrane 10,11,[27][28][29][30] have treated the zwitterionic lipids simply as being neutral, i.e., without bearing any electrostatic moments. In the present work we adopt a recently suggested model 26 where the head-FIG.…”

Section: Theorymentioning

confidence: 99%

The influence of zwitterionic lipids on the electrostatic adsorption of macroions onto mixed lipid membranes

Haugen

May

2007

The Journal of Chemical Physics

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Charged lipid membranes commonly consist of a mixture of charged and zwitterionic lipids. We suggest a model that characterizes the influence of the dipolar nature of the zwitterionic lipid species on the electrostatic adsorption of macroions onto mixed membranes in the fluid state. The model is based on Poisson-Boltzmann theory which we have modified so as to account for the dipolar character of the zwitterionic lipids. In addition the membrane lipids are allowed to adjust their lateral distribution upon macroion adsorption. We consider and compare two experimentally relevant scenarios: cationic macroions adsorbed onto anionic membranes and anionic macroions adsorbed onto cationic membranes. We show that in the former case the adsorption strength is slightly weakened by the presence of the headgroup dipoles of the zwitterionic lipids. Here, macroion-induced lipid demixing is more pronounced and the lipid headgroups tilt away from a cationic macroion upon adsorption. In contrast, for the adsorption of anionic macroions onto a cationic membrane the zwitterionic lipids strongly participate in the electrostatic interaction between membrane and macroion, thus enhancing the adsorption strength significantly (we predict up to 20%). Consistent with that we find less lateral demixing of the charged lipids and a reorientation of the dipoles of the zwitterionic headgroups towards the anionic macroions. Our results may be of importance to understand the differences in the electrostatic adsorption of proteins/peptides onto cellular membranes versus complex formation between cationic membranes and DNA.

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Physical Basis for Membrane-Charge Selectivity of Cationic Antimicrobial Peptides

Cited by 27 publications

References 15 publications

d -Alanylation of Lipoteichoic Acid Contributes to the Virulence of Streptococcus suis

d -Alanylation of Lipoteichoic Acid Contributes to the Virulence of Streptococcus suis

Cationic peptide-induced remodelling of model membranes: Direct visualization by in situ atomic force microscopy

The influence of zwitterionic lipids on the electrostatic adsorption of macroions onto mixed lipid membranes

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