Physical and Natural Crosslinking Approaches on Three-Dimensional Gelatin Microspheres for Cartilage Regeneration

Sulaiman, Shamsul; Rani, Rizal Bin Abdul; Yahaya, Nor Hamdan Bin Mohamad; Tabata, Yasuhiko; Hiraoka, Yosuke; Seet, Wan Tai; Ng, Angela Min Hwei

doi:10.1089/ten.tec.2022.0073

Cited by 3 publications

(1 citation statement)

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“…3D cell aggregates have been widely used as a tool to mimic the in vivo environment. ,− However, due to the lack of oxygen and nutrition, the cells located in the center of aggregates tend to die. To break through this problem, GMS are effective in functioning as a cell scaffold, which allows cells to supply oxygen and nutrition. ,− On the other hand, GMS are a good carrier for biomolecules, such as low molecular-weight drug, protein, and siRNA, to achieve the controlled release. − Increasing biological functions in cell aggregates would be achieved by preparing cell aggregate with biomolecules loaded GMS.…”

Section: Discussionmentioning

confidence: 99%

A Reverse Transfection System with Cationized Gelatin Nanospheres Incorporating Molecular Beacon as a Tool to Visualize Cell Function

Yang

Tabata

2023

ACS Appl. Bio Mater.

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The objective of this research is to design a reverse transfection system with cationized gelatin nanospheres (cGNS) incorporating a molecular beacon (MB) to visualize a cell function. The cGNS were prepared by the conventional coacervation method. The MB as an imaging probe was incorporated into the cGNS to prepare imaging complexes (cGNS MB ). The conventional transfection of 2D culture was performed by incubating MC3T3 cells in the medium containing cGNS MB . The reverse transfection was done by incubating cells on the substrate which had been precoated with both gelatin and cGNS MB . Significantly higher internalization efficiency and fluorescence intensity of cGNS MB were observed in the reverse transfection system than in the conventional one. To apply this system for visualization of 3D cell aggregate, gelatin microspheres (GMS) were prepared, while cGNS MB were bound on the GMS to prepare the GMS-cGNS MB of a cell scaffold. Then the cells were incubated with GMS-cGNS MB to form 3D cell aggregates. On the other hand, as a control, the conventional transfection of 3D culture was performed by incubating the cell aggregates formed with the medium containing cGNS MB . Homogeneous fluorescence of MB from the inside to the outside of aggregates was observed for the reverse transfection group. However, for the conventional transfection, the fluorescence was observed only around the surface of cell aggregates. It is concluded that the reverse transfection system with cGNS incorporating MB is promising to visualize the cell function of a higher transfection efficiency for the 2D culture and in a homogeneous manner for the 3D culture.

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Section: Discussionmentioning

confidence: 99%

A Reverse Transfection System with Cationized Gelatin Nanospheres Incorporating Molecular Beacon as a Tool to Visualize Cell Function

Yang

Tabata

2023

ACS Appl. Bio Mater.

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Engineered hydrogel microspheres for spheroids and organoids construction

Gai,

Zhang,

et al. 2024

Chemical Engineering Journal

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Articular Cartilage Regeneration through Bioassembling Spherical Micro-Cartilage Building Blocks

Grottkau¹,

Hui²,

Pang³

2022

Cells

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Articular cartilage lesions are prevalent and affect one out of seven American adults and many young patients. Cartilage is not capable of regeneration on its own. Existing therapeutic approaches for articular cartilage lesions have limitations. Cartilage tissue engineering is a promising approach for regenerating articular neocartilage. Bioassembly is an emerging technology that uses microtissues or micro-precursor tissues as building blocks to construct a macro-tissue. We summarize and highlight the application of bioassembly technology in regenerating articular cartilage. We discuss the advantages of bioassembly and present two types of building blocks: multiple cellular scaffold-free spheroids and cell-laden polymer or hydrogel microspheres. We present techniques for generating building blocks and bioassembly methods, including bioprinting and non-bioprinting techniques. Using a data set of 5069 articles from the last 28 years of literature, we analyzed seven categories of related research, and the year trends are presented. The limitations and future directions of this technology are also discussed.

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Physical and Natural Crosslinking Approaches on Three-Dimensional Gelatin Microspheres for Cartilage Regeneration

Cited by 3 publications

References 50 publications

A Reverse Transfection System with Cationized Gelatin Nanospheres Incorporating Molecular Beacon as a Tool to Visualize Cell Function

A Reverse Transfection System with Cationized Gelatin Nanospheres Incorporating Molecular Beacon as a Tool to Visualize Cell Function

Engineered hydrogel microspheres for spheroids and organoids construction

Articular Cartilage Regeneration through Bioassembling Spherical Micro-Cartilage Building Blocks

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