Physical and <i>in vitro</i> biological evaluation of a PA 6/MWCNT electrospun composite for biomedical applications

Volpato, Fabio Zomer; Ramos, S.; Motta, Antonella; Migliaresi, Claudio

doi:10.1177/0883911510391449

Cited by 31 publications

(32 citation statements)

References 37 publications

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“…The CNT was expected to form hydrogen bonds between the polymer and filler or form amide bonds among free amines on the polymer and the CNT’s carboxyl groups [54]. The physiochemical properties of PU have been improved by the inclusion of CNTs.…”

Section: Nanocomposites For Improving the Mechanical Strength Of Prosmentioning

confidence: 99%

Tangible nanocomposites with diverse properties for heart valve application

Vellayappan

Balaji

Subramanian

et al. 2015

Science and Technology of Advanced Materials

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Cardiovascular disease claims millions of lives every year throughout the world. Biomaterials are used widely for the treatment of this fatal disease. With the advent of nanotechnology, the use of nanocomposites has become almost inevitable in the field of biomaterials. The versatile properties of nanocomposites, such as improved durability and biocompatibility, make them an ideal choice for various biomedical applications. Among the various nanocomposites, polyhedral oligomeric silsesquioxane-poly(carbonate-urea)urethane, bacterial cellulose with polyvinyl alcohol, carbon nanotubes, graphene oxide and nano-hydroxyapatite nanocomposites have gained popularity as putative choices for biomaterials in cardiovascular applications owing to their superior properties. In this review, various studies performed utilizing these nanocomposites for improving the mechanical strength, anti-calcification potential and hemocompatibility of heart valves are reviewed and summarized. The primary motive of this work is to shed light on the emerging nanocomposites for heart valve applications. Furthermore, we aim to promote the prospects of these nanocomposites in the campaign against cardiovascular diseases.

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Section: Nanocomposites For Improving the Mechanical Strength Of Prosmentioning

confidence: 99%

Tangible nanocomposites with diverse properties for heart valve application

Vellayappan

Balaji

Subramanian

et al. 2015

Science and Technology of Advanced Materials

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“…We used FG-coated PEA fibers obtained via electrospinning to demonstrate the potential of cellular interaction in 3D of these scaffolds for tissue engineering applications. 26,27 Materials and methods Preparation of ethyl acrylate (EA) and hydroxyethyl acrylate (HEA) copolymers…”

Section: Introductionmentioning

confidence: 99%

Fibrinogen organization at the cell-material interface directs endothelial cell behavior

Gugutkov

González-García

Altankov

et al. 2011

Journal of Bioactive and Compatible Polymers

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Fibrinogen (FG) adsorption on surfaces with controlled fraction of ÀOH groups was investigated with AFM and correlated to the initial interaction of primary endothelial cells (HUVEC). The ÀOH content was tailored making use of a family of copolymers consisting of ethyl acrylate (EA) and hydroxyl ethyl acrylate (HEA) in different ratios. The supramolecular distribution of FG changed from an organized network-like structure on the most hydrophobic surface (ÀOH 0 ) to dispersed molecular aggregate one as the fraction of ÀOH groups increases, indicating a different conformation by the adsorbed protein. The best cellular interaction was observed on the most hydrophobic (ÀOH 0 ) surface where FG assembled in a fibrin-like appearance in the absence of any thrombin. Likewise, focal adhesion formation and actin cytoskeleton development was poorer as the fraction of hydroxy groups on the surface was increased. The biological activity of the surfaceinduced FG network to provide 3D cues in a potential tissue engineered scaffold, making use of electrospun PEA fibers (ÀOH 0 ), seeded with human umbilical vein endothelial cells was investigated. The FG assembled on the polymer fibers gave rise to a biologically active network able to direct cell orientation along the fibers (random or aligned), promote cytoskeleton organization and focal adhesion formation.Keywords fibrinogen, cell-material interactions, HUVEC, electrospun fibers, fibrinogen organization, cell-material interface, endothelial cell behavior, ethyl acrylate, hydroxyl ethyl acrylate Journal of Bioactive and Compatible Polymers 26(4) 375-387

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“…The second one underlies the epidermis, and is composed of the predominating extracellular matrix (ECM) proteins such as collagen, elastin, and glycosaminoglycans, and the cellular constituents of mainly fibroblasts. [6][7][8][9][10][11] This technique produces nonwoven membranes with individual fiber diameters ranging from a few nanometers to hundreds of nanometers, which mimics the structure and the function of natural ECM. 2 Dermal wounds caused by mechanical trauma, surgical procedures, burns, chemicals, or diseases are conventionally treated using autografts, allografts, and xenografts.…”

Section: Introductionmentioning

confidence: 99%

Fibrous star poly(ε-caprolactone) melt-electrospun scaffolds for wound healing applications

Gazzarri

Bartoli

Mota

et al. 2013

Journal of Bioactive and Compatible Polymers

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Polymeric fibrous scaffolds based on the biocompatible and biodegradable three-arm-branched star poly(ε-caprolactone) (Mw = 189,000 g/mol) were prepared by a melt electrospinning technique. The possibility of processing polymers without the use of organic solvents is one of the main advantages over solution electrospinning. Scaffolds were biologically tested for their ability of supporting skin tissue regeneration. For this purpose, mouse embryo fibroblast (BALB/3T3 clone A31) and human keratinocyte (HaCaT) cell lines were selected as models, and seeded onto the polymeric supports both as single and co-culture. Cell viability, proliferation, and collagen production were assessed by WST-1 assay and Direct Red 80 dye, respectively. Cell morphology and colonization of the supports were evaluated by scanning electron microscopy and confocal laser scanning microscopy. Results highlighted that the star poly(ε-caprolactone) scaffolds were able to promote collagen production by fibroblasts. In co-culture studies, scaffolds supported adhesion, proliferation, and spatial organization of both cell lines. By virtue of the observed results, the developed polymeric scaffolds appeared suitable as biodegradable and biocompatible three-dimensional supports for skin tissue regeneration in wound healing dressing.

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Physical and in vitro biological evaluation of a PA 6/MWCNT electrospun composite for biomedical applications

Cited by 31 publications

References 37 publications

Tangible nanocomposites with diverse properties for heart valve application

Tangible nanocomposites with diverse properties for heart valve application

Fibrinogen organization at the cell-material interface directs endothelial cell behavior

Fibrous star poly(ε-caprolactone) melt-electrospun scaffolds for wound healing applications

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