Physical and genetic map of the Bacteroides fragilis YCH46 chromosome

Abstract

The chromosome of Bacteroides fragilis strain YCH46 was shown to be a single circular DNA molecule of about 5.3 Mb having 16 NotI, seven AscI, and six I-CeuI sites. A physical map of the chromosome was constructed by four independent experimental approaches: linking clone analysis, cross-Southern hybridization, partial restriction digestion, and two-dimensional pulsed-field gel electrophoresis. Six rRNA operons and 10 known genes were localized on the physical map. ß

The Medically Important Bacteroides spp. in Health and Disease

“…Overall the average size range of the Bacteroides genomes is similar to the median size of the known eubacterial genomes that are already or in the process of being sequenced (see TIGR in progress and TIGR complete websites). A chromosome map of one B. fragilis strain (homology group II strain YCH46) has been constructed for the enzymes NotI (16 sites), AscI (7 sites), and CeuI (6 sites; Kuwahara et al, 2002). The chromosome was circular and approximately 5.3 Mb.…”

Development of a physical map for three Frankia strains and a partial genetic map for Frankia EuI1c

“…The intron‐encoded restriction endonuclease, I‐ Ceu I, recognizes a highly conserved 19‐bp sequence within rrl genes for the large rRNA subunit (23S) gene (Liu et al 1993) and has been used as an indicator for the number of rRNA ( rrn ) operons in a genome (Dudez et al 2002, Kuwahara et al 2002). Digestion of the Frankia EuI1c genome with I‐ Ceu I produced three DNA fragments, which suggests the presence of three rRNA operons in Frankia EuI1c chromosome.…”