Physical and gene organization of mitochondrial DNA from the fertile cytoplasm of sugarbeet (Beta vulgaris L.)

Kubo, Tomohiko; Satoh, Yuka; Muro, Takato; Kinoshita, Toshiro; Mikami, Tetsuo

doi:10.1007/bf00309782

Cited by 20 publications

(17 citation statements)

References 48 publications

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“…orientalis acc. BGRC56777 (seq01 haplotype) and compared it with the other known sugar beet mtDNA maps (Kubo et al 1995(Kubo et al , 1999. BGRC56777 was chosen for mapping because this accession was found to be similar but not identical to cv.…”

Section: Resultsmentioning

confidence: 99%

“…TK81-O and cv. TK81-MS (Kubo et al 1995(Kubo et al , 1999, respectively, were sequentially used as probes against Southern blots prepared with total genomic DNA from BGRC56777. This enabled us to identify a number of regions that were unchanged in hybridization patterns either between BGRC56777 and TK81-O or between BGRC56777 and TK81-MS, but that were delimited by rearrangement breakpoints.…”

Section: Resultsmentioning

confidence: 99%

“…DNA fragments of 18 kbp were fractionated by centrifugation and cloned into the Lambda DASH II vector (Stratagene) according to standard procedures. All other library manipulations were as described by Kubo et al (1995Kubo et al ( , 1999. DNA fragments were labeled using Gene Images (GE Healthcare Bio-Sciences, Piscataway, NJ).…”

Section: Methodsmentioning

confidence: 99%

“…Families of overlapping phages were identified by screening the acc. BGRC56777 mtDNA library with the subfragments of TK81-O mtDNA (Kubo et al 1995) or TK81-MS mtDNA (Kubo et al 1999). The restriction maps of the phage clones identified were determined and further extended by genome walking from the extremities of the isolated groups of phages.…”

Section: Methodsmentioning

confidence: 99%

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Mitochondrial DNA Phylogeny of Cultivated and Wild Beets: Relationships Among Cytoplasmic Male-Sterility-Inducing and Nonsterilizing Cytoplasms

Nishizawa¹,

Mikami

Kubo

2007

Genetics

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Cytoplasmic male sterility (CMS), the maternally inherited failure to produce functional pollen, has been used in the breeding of sugar beet (Beta vulgaris ssp. vulgaris). At least three different sources of CMS can be distinguished from one another as well as from normal fertile cytoplasm by polymorphisms in their mitochondrial genomes. Here we analyzed 50 accessions of cultivated and wild beets to investigate the phylogenetic relationships among male-sterility-inducing and normal cytoplasms. The haplotypes were characterized by the nucleotide sequence of the mitochondrial cox2-cox1 spacer region and mitochondrial minisatellite loci. The results indicated that (1) a normal cytoplasm line, cv. TK81-O, was situated at the major core node of the haplotype network, and (2) the three sterilizing cytoplasms in question derived independently from the core haplotype. The evolutionary pathway was investigated by physical mapping study of the mitochondrial genome of a wild beet (B. vulgaris ssp. orientalis) accession BGRC56777 which shared the same mitochondrial haplotype with TK81-O, but was not identical to TK81-O for the RFLP profiles of mitochondrial DNA. Interestingly, three sets of inverted repeated sequences appeared to have been involved in a series of recombination events during the course of evolution between the BGRC56777 and the TK81-O mitochondrial genomes.T HE involvement of cytoplasmic factors has been reported in .150 plant species in which male sterility occurs (Laser and Lersten 1972). This type of male sterility, called cytoplasmic male sterility (CMS), is usually attributed to chimeric ORFs in the mitochondrial genome (Chase 2007). In some plant species, multiple forms of CMS are found (Fauron and Casper 1994;Schnable and Wise 1998). These forms can be distinguished by their unusual associated mitochondrial ORFs and by their response to nuclear-encoded, restorer-of-fertility (Rf ) genes that suppress the malesterile phenotype.Several different sources of male-sterile cytoplasms have been described in cultivated beets (Beta vulgaris ssp. vulgaris) and their wild progenitor, all of which are assigned into the section Beta (syn. Vulgares) of the genus Beta (Mikami et al. 1985;Saumitou-Laprade et al. 1993). The first identified was the Owen cytoplasm, discovered in the cultivar ''US-1'' (Owen 1945). Up to now hybrid seed production in the sugar beet has exclusively relied on Owen CMS (Bosemark 1993).We have recently found that a mitochondrial 35-kDa protein is most likely responsible for Owen CMS (Satoh et al. 2004; Yamamotoet al. 2005). The protein proved to be the product of a 387-codon mitochondrial ORF (designated preSatp6), which is fused in frame with the downstream atp6 ORF (Yamamoto et al. 2005). Interestingly, this protein was not detectable in the anther tissue of two different sources of CMS ½I-12CMS(2) and I-12CMS (3) from wild Beta beets collected in Turkey and Pakistan; instead, a CMS-associated protein of 12 kDa was identified (Hallden et al. 1992; M. P. Yamamoto, Y. Onodera, T. Ku...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Mitochondrial DNA Phylogeny of Cultivated and Wild Beets: Relationships Among Cytoplasmic Male-Sterility-Inducing and Nonsterilizing Cytoplasms

Nishizawa¹,

Mikami

Kubo

2007

Genetics

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“…One involved a small fragment, sites 840-843, similar to recombination in organelle DNA of other plants (Lonsdale et al 1988;Kubo et al 1995). A second large fragment crossover between sites 539-840 was detected in Hygrophila pogonocalyx.…”

Section: Genetic Diversity and Cpdna Phylogeny Of Hygrophila Pogonocalyxmentioning

confidence: 94%

Phylogeography and conservation genetics of Hygrophila pogonocalyx (Acanthaceae) based on atpB?rbcL noncoding spacer cpDNA

Huang¹,

Wang

Peng

et al. 2005

J Plant Res

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Genetic variation in the atpB-rbcL intergenic spacer region of chloroplast DNA (cpDNA) was investigated in Hygrophila pogonocalyx Hayata (Acanthaceae), an endangered and endemic species in Taiwan. In this aquatic species, seed dispersal from capsules via elasticity is constrained by gravity and is thereby confined within populations, resulting in limited gene flow between populations. In this study, a total of 849 bp of the cpDNA atpB-rbcL spacer were sequenced from eight populations of H. pogonocalyx. Nucleotide diversity in the cpDNA is low (theta = 0.00343+/-0.00041). The distribution of genetic variation among populations agrees with an "isolation-by-distance" model. Two geographically correlated groups, the western and eastern regions, were identified in a neighbor-joining tree and a minimum-spanning network. Phylogeographical analyses based on the cpDNA network suggest that the present-day differentiation between western and eastern groups of H. pogonocalyx resulted from past fragmentation. The differentiation between eastern and western populations may be ascribed to isolation since the formation of the Central Mountain Range about 5 million years ago, which is consistent with the rate estimates based on a molecular clock of cpDNA.

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Extranuclear Inheritance: Genetics and Biogenesis of Mitochondria

et al. 1997

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Physical and gene organization of mitochondrial DNA from the fertile cytoplasm of sugarbeet (Beta vulgaris L.)

Cited by 20 publications

References 48 publications

Mitochondrial DNA Phylogeny of Cultivated and Wild Beets: Relationships Among Cytoplasmic Male-Sterility-Inducing and Nonsterilizing Cytoplasms

Mitochondrial DNA Phylogeny of Cultivated and Wild Beets: Relationships Among Cytoplasmic Male-Sterility-Inducing and Nonsterilizing Cytoplasms

Phylogeography and conservation genetics of Hygrophila pogonocalyx (Acanthaceae) based on atpB?rbcL noncoding spacer cpDNA

Extranuclear Inheritance: Genetics and Biogenesis of Mitochondria

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