Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain

Zhu, Wenjing; Wang, Yuzhen; Fang, Yan; Song, Ruiteng; Z, Li; Li, Yiqing; Song, Bo

doi:10.1016/j.carbpol.2018.03.063

Cited by 23 publications

(13 citation statements)

References 32 publications

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“…It has been reported that EPS can hydrate the stratum corneum of the skin, thereby changing the stratum corneum of the epidermis and enlarging the pores to increase the percutaneous penetration of the drug. This mechanism for the enhancement of its penetration is relatively safer than the use of a penetration enhancer, such as dimethyl sulfoxide [27]. EPS is a natural non-toxic and non-irritating polysaccharide with good biocompatibility.…”

Section: Discussionmentioning

confidence: 99%

Preparation of Calcipotriol Emulsion Using Bacterial Exopolysaccharides as Emulsifier for Percutaneous Treatment of Psoriasis Vulgaris

Song

Cheng

et al. 2019

IJMS

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An exopolysaccharides/calcipotriol (EPS/CPT) emulsion was prepared using bacterial EPS as emulsifier, sunflower oil as an oil phase and CPT as the loaded drug, and the effect of this emulsion on psoriasis vulgaris treatment was evaluated. An EPS composed of mannose (70.56%) and glucose (29.44%) was obtained from the marine mangrove bacteria Bacillus amyloliquefaciens ZWJ (Zhu Wenjing) strain. The EPS has significant emulsifying activity at the concentration of 1.5%. The prepared EPS/CPT emulsion has small and stable particle size, with a drug content of 0.00492%, and good spreading properties. The in vitro drug release results revealed that the emulsion showed a certain sustained release effect. In vitro and in vivo animal experiments show that the EPS/CPT emulsion can effectively treat psoriasis vulgaris by increasing the accumulation of CPT in psoriatic skin lesions and reducing the levels of inflammatory cells and inflammatory factors (TNF and IL6). Additionally, it has a certain effect on reducing the side effects associated with CPT. This study lays a foundation for the research of EPS in the topical application of medical materials and treatment of psoriasis.

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Section: Discussionmentioning

confidence: 99%

Preparation of Calcipotriol Emulsion Using Bacterial Exopolysaccharides as Emulsifier for Percutaneous Treatment of Psoriasis Vulgaris

Song

Cheng

et al. 2019

IJMS

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“…The viscous substance was presumed to be exopolysaccharides (EPS) or polyglutamic acid (PGA) according to previous research [24][25][26][27], and it was identified via gas chromatography-mass spectrometry (GC/MS) (Agilent, USA). Before GC/MS detection, the samples were treated as follows: The supernatant of the fermentation broth was successively collected, diluted, monomeric sugars removed by ultrafiltration, and the interception was combined with a triple volume of 70% ethanol for 30 min.…”

Section: Analytic Methodsmentioning

confidence: 99%

Optimized expression and enhanced production of alkaline protease by genetically modified Bacillus licheniformis 2709

Zhou

et al. 2020

Microb Cell Fact

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Background: Bacillus licheniformis 2709 is extensively applied as a host for the high-level production of heterologous proteins, but Bacillus cells often possess unfavorable wild-type properties, such as production of viscous materials and foam during fermentation, which seriously influenced the application in industrial fermentation. How to develop it from a soil bacterium to a super-secreting cell factory harboring less undomesticated properties always plays vital role in industrial production. Besides, the optimal expression pattern of the inducible enzymes like alkaline protease has not been optimized by comparing the transcriptional efficiency of different plasmids and genomic integration sites in B. licheniformis. Result: Bacillus licheniformis 2709 was genetically modified by disrupting the native lchAC genes related to foaming and the eps cluster encoding the extracellular mucopolysaccharide via a markerless genome-editing method. We further optimized the expression of the alkaline protease gene (aprE) by screening the most efficient expression system among different modular plasmids and genomic loci. The results indicated that genomic expression of aprE was superior to plasmid expression and finally the transcriptional level of aprE greatly increased 1.67-fold through host optimization and chromosomal integration in the vicinity of the origin of replication, while the enzyme activity significantly improved 62.19% compared with the wild-type alkaline protease-producing strain B. licheniformis. Conclusion: We successfully engineered an AprE high-yielding strain free of undesirable properties and its fermentation traits could be applied to bulk-production by host genetic modification and expression optimization. In summary, host optimization is an enabling technology for improving enzyme production by eliminating the harmful traits of the host and optimizing expression patterns. We believe that these strategies can be applied to improve heterologous protein expression in other Bacillus species.

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“…It is a Gram-positive bacterium with elongated rod-shaped cells that interact with one another to generate a significant quantity of biofilm as an aggregate. Based on morphological (Appendix A), physico-chemical (Appendix B), and biochemical (Appendix C) tests, the bacterium, which had spore-forming ability and facultative anaerobic metabolism, was classified within the genus Bacillus, and it may further be catalogued as either B. subtilis or B. atrophaeus [32,33]. Next-generation sequencing will be conducted to complete the identification.…”

Section: Microorganism Isolation and Culture Conditionsmentioning

confidence: 99%

Exopolysaccharides Production by Cultivating a Bacterial Isolate from the Hypersaline Environment of Salar de Uyuni (Bolivia) in Pretreatment Liquids of Steam-Exploded Quinoa Stalks and Enzymatic Hydrolysates of Curupaú Sawdust

et al. 2021

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The halotolerant bacterial strain BU-4, isolated from a hypersaline environment, was identified as an exopolysaccharide (EPS) producer. Pretreatment liquids of steam-exploded quinoa stalks and enzymatic hydrolysates of Curupaú sawdust were evaluated as carbon sources for EPS production with the BU-4 strain, and the produced EPS was characterized using FTIR, TGA, and SEM. Cultivation was performed at 30 °C for 48 h, and the cells were separated from the culture broth by centrifugation. EPS was isolated from the cell pellets by ethanol precipitation, and purified by trichloroacetic acid treatment, followed by centrifugation, dialysis, and freeze-drying. EPS production from quinoa stalks- and Curupaú sawdust-based substrates was 2.73 and 0.89 g L−1, respectively, while 2.34 g L−1 was produced when cultivation was performed on glucose. FTIR analysis of the EPS revealed signals typical for polysaccharides, as well as ester carbonyl groups and sulfate groups. High thermal stability, water retention capacity and gel-forming ability were inferred from SEM and TGA. The capability of the halotolerant isolate for producing EPS from pretreatment liquids and hydrolysates was demonstrated, and characterization of the EPS revealed their broad application potential. The study shows a way for producing value-added products from waste materials using a bacterium from a unique Bolivian ecosystem.

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Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain

Cited by 23 publications

References 32 publications

Preparation of Calcipotriol Emulsion Using Bacterial Exopolysaccharides as Emulsifier for Percutaneous Treatment of Psoriasis Vulgaris

Preparation of Calcipotriol Emulsion Using Bacterial Exopolysaccharides as Emulsifier for Percutaneous Treatment of Psoriasis Vulgaris

Optimized expression and enhanced production of alkaline protease by genetically modified Bacillus licheniformis 2709

Exopolysaccharides Production by Cultivating a Bacterial Isolate from the Hypersaline Environment of Salar de Uyuni (Bolivia) in Pretreatment Liquids of Steam-Exploded Quinoa Stalks and Enzymatic Hydrolysates of Curupaú Sawdust

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