Physical and chemical properties of a protein isolated from red cell membranes

Isolation and identification of an actin from rat liver

“…The membrane fraction of 8 rat livers (40 g wet weight) yielded by extraction with EDTA and lyophflization [8] 5 mg proteins which gave 1 mg of a protein fraction by affinity chromatography on elution in presence of 3 M guanidine-HCl (peak V in , '7 ..... , "". fig.l) and subsequent dialysis against twice-distilled water.…”

“…After washing the light membrane free of sucrose with twice-distilled water, proteins were extracted with EDTA according to Marchesi et al [8]. The extract was lyophilized and the protein purified on a column of DNase-agarose according to the method of Lazarides and Lindberg [5].…”

“…32/aCi/ mg) 20 min before sacrifice. After dialysis of this extract against twice-distilled water and lyophilization, it was chromatographed on a Sephadex G-200 column as described by Marchesi et al [8]. Radioactivity was measured in a Packard Tricarb Scintillation Counter.…”

Rotational dynamics of spectrin in solution and ankyrin bound in human erythrocyte membrane

“…Spectrin concentration was calculated on a basis of MW 460 000 and E.$$o = 10.1 [9,10]. The maleimide spin-labeled spectrin (MSL-spectrin) was prepared with 3-maleimido-2,2',5,5' tetramethyl-l-pyrrolidinyloxyl from Syva as in [5].…”

Extraction of erythrocyte membrane proteins by sulfhydryl inhibitors.

“…The components 1 and 2 have been originally called spectrin because they are obtained from ghosts (Marchesi and Steers Jr. 1968) and amount to about 20 to 25% of the total membrane proteins (Singer 1974). Spectrin is considered to form a fibrous structure on the inner surface of the membrane and has been implicated in mainta ining the shape and integrity of the red cell (Marchesi and Steers Jr. 1968; Marchesi et al 1969). Recent observations have indicated an intimate association betweenn spectrin and the intramembranous particles (Elgsaeter and Branton 1974;Tilney and Detmers 1975;Elgsaeter et al 1976).…”