Physical and chemical characterization of adsorbed protein onto gold electrode functionalized with Tunisian coral and nacre

Hamza, Samir; Bouchemi, Meryem; Slimane, N.; Azari, Z.

doi:10.1016/j.msec.2012.09.028

Cited by 4 publications

(9 citation statements)

References 41 publications

(40 reference statements)

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“…A previous study has shown that nacre was able to form a tight bond to bone without soft or fibrous tissue intervening, and the material slowly dissolved away [4]. Human in vitro studies on osteoblasts have demonstrated the ability of nacre to induce mineralised tissue formation [5, 6]. The potential use of coral minerals was reported in scaffold formation by the system of bone forming and desorbing cells, which remodelled over a long period of time [7].…”

Section: Introductionmentioning

confidence: 99%

“…The potential use of coral minerals was reported in scaffold formation by the system of bone forming and desorbing cells, which remodelled over a long period of time [7]. Coralline minerals are biocompatible and have been used as bone substitutes due to their unique and excellent properties, such as osteoconductive quality and high-speed in bone resorption, and since they do not transmit infectious diseases [5]. Furthermore, coral mineral implants have shown an excellent tolerance, osteointegration, osteoconduction, and progressive resorption with replacement by neoformed bone tissue [5, 6].…”

Section: Introductionmentioning

confidence: 99%

“…No single type of material is able to mimic the composition, structure, and properties of native bone. However, consider the role played by biomaterials derived from nature, such as nacre, pearl, and coral, in development and their uses in bone scaffold and the repair of bone defects as cited in the literature [5, 7]. Therefore, the synthesis of nanocomposites from these biomaterials may be the best choice for bone tissue regeneration since they can provide the appropriate matrix environment, integrate desirable biological properties, and provide controlled, sequential delivery of multiple growth factors for different stages of bone tissue regeneration [8].…”

Section: Introductionmentioning

confidence: 99%

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Osteoblasts Growth Behaviour on Bio-Based Calcium Carbonate Aragonite Nanocrystal

Kamba

Zakaria

2014

BioMed Research International

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Calcium carbonate (CaCO3) nanocrystals derived from cockle shells emerge to present a good concert in bone tissue engineering because of their potential to mimic the composition, structure, and properties of native bone. The aim of this study was to evaluate the biological response of CaCO3 nanocrystals on hFOB 1.19 and MC3T3 E-1 osteoblast cells in vitro. Cell viability and proliferation were assessed by MTT and BrdU assays, and LDH was measured to determine the effect of CaCO3 nanocrystals on cell membrane integrity. Cellular morphology was examined by SEM and fluorescence microscopy. The results showed that CaCO3 nanocrystals had no toxic effects to some extent. Cell proliferation, alkaline phosphatase activity, and protein synthesis were enhanced by the nanocrystals when compared to the control. Cellular interactions were improved, as indicated by SEM and fluorescent microscopy. The production of VEGF and TGF-1 was also affected by the CaCO3 nanocrystals. Therefore, bio-based CaCO3 nanocrystals were shown to stimulate osteoblast differentiation and improve the osteointegration process.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Osteoblasts Growth Behaviour on Bio-Based Calcium Carbonate Aragonite Nanocrystal

Kamba

Zakaria

2014

BioMed Research International

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“…It has been used as bone substitutes for high-speed bone resorption and for osteoconductive quality. (14) Effect of calcium carbonate coating Mechanical testing:…”

Section: Discussionmentioning

confidence: 99%

Assessment of Calcium Carbonate Coating on Osseointegration of Commercially Pure Titanium Implant by Torque Removal Test and Histomorphometric Analysis

Mahmood¹,

Al-Ameer²

2017

JBCD

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Background: One of the most important methods to replace lost teeth is dental implants. In order to increase the strength of connection of the implant with the jaw bone to provide early loading after placement, implant is coated by different coating materials that achieved that purpose. The aim of this study was to evaluate the influence of coating CP Ti implant with calcium carbonate on the strength of bone-implant interface after two and six weeks of implantation in rabbit femur bone by torque removal test, histological and histomorphometric analysis. Materials and methods: Coating the surface of commercially pure titanium screws with extra pure synthetic calcium carbonate via electrophoretic deposition method (EPD) was done. The surface of disc samples after coating was checked by optical microscopy, X-ray diffraction examination and measurement of coating thickness. Ten male white French rabbits were prepared for implantation. Forty screws were implanted in the femur bone, two implant screws in each femur bone. The first screw is coated with calcium carbonate and compared with the second uncoated screw. Rabbits are divided into two groups according to the healing periods 2 and 6 weeks. By torque removal, the osseointegration is measured. Single screw from each group was used for histological and Histomorphometric analysis. Results: There was significant increased mean torque removal for screws coated with calcium carbonate compared with uncoated screws. Histological examination showed an increase in the growth of bone cells for coated screws, and the histomorphometric analysis showed an increase in new bone formation percent (NBFP). Conclusion: Coating the surface of the CP Ti implant with calcium carbonate via electrophoretic deposition method had great effect in increasing the osseointegration than uncoated surface.

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“…It is a natural composite material consisting of an inorganic mineral phase and an organic matrix, similar to the structure of human bone. This organized mineral structure is related to its remarkable mechanical strength, while the presence of the organic matrix imparts improved osteoconductivity [ 23 ]. The organic matrix has also been found to contain biological molecules practically identical to those found in humans, the BMPs (bone morphogenetic proteins) [ 27 ], and other molecules capable of activating osteoblasts through chemical signaling.…”

Section: Introductionmentioning

confidence: 99%

Bone substitute made from a Brazilian oyster shell functions as a fast stimulator for bone-forming cells in an animal model

et al. 2018

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Despite their demonstrated biocompatibility and osteogenic properties, oyster shells have been reported as a potential alternative to other commonly used materials for bone substitution. This study evaluated whether an experimental bone substitute (EBS) made from a typical oyster shell of Northeastern Brazil (Crassostrea rhizophora) has effects on bone development using an animal model. Oysters were collected from a biologically assisted vivarium, and their inner layer was used for preparing an EBS. Chemical and surface characterization of EBS was performed using Individually Coupled Plasma Optical Emission Spectrometry (ICP-OES) and Scanning Electron Microscope (SEM), respectively. Seventy-two rats were randomly assigned to groups according to the treatment of bone defects created in the submandibular area: Negative Control (-C), Positive Control (+C; Bio-Oss®) and EBS. Euthanasia occurred at 7, 21, 42 and 56 days postoperatively. The bone pieces were stained with hematoxylin and eosin (H&E). The formation of bone tissue was evaluated histologically and histomorphometrically. Data were analyzed through the Kruskal-Wallis test and ANOVA considering a significant level of 5%. The main element found in EBS was calcium (71.68%), and it presented heterogeneity in the particle size and a porosity aspect at SEM analysis. Histological results revealed the absence of inflammatory cells in all groups, being that EBS presented the most accelerated process of bone formation with a statistically significant difference between this group and the +C and -C groups in the 21-day time-point (p < 0.05). After 21 days, the bone formation process was similar between all groups (p > 0.05), showing an immature lamellar bone pattern after 56 days of experimentation (p > 0.05). Within the limitations of this study, it was possible to conclude that EBS presented good biocompatibility and promoted fast stimulation for bone-forming cells in an animal model.

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Physical and chemical characterization of adsorbed protein onto gold electrode functionalized with Tunisian coral and nacre

Cited by 4 publications

References 41 publications

Osteoblasts Growth Behaviour on Bio-Based Calcium Carbonate Aragonite Nanocrystal

Osteoblasts Growth Behaviour on Bio-Based Calcium Carbonate Aragonite Nanocrystal

Assessment of Calcium Carbonate Coating on Osseointegration of Commercially Pure Titanium Implant by Torque Removal Test and Histomorphometric Analysis

Bone substitute made from a Brazilian oyster shell functions as a fast stimulator for bone-forming cells in an animal model

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