Physical and chemical characterization of RNA incorporated by rabbit spleen cells

Wang, S.R.; Giacomoni, Dario; Dray, Sheldon

doi:10.1016/0014-4827(73)90032-3

Cited by 17 publications

(6 citation statements)

References 18 publications

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“…Незначительное снижение удельной радиоактивности клеток после многократного отмывания и обработки панкреатической рибонуклеазой свидетельствовало о том, что экзогенная РНК действительно поглощалась лимфоцитами, а не адсорбировалась на их мембране. Так же быстро в лимфоидные клетки селезенки проникала гомологичная суммарная РНК, стимулируя при этом их синтетическую активность [11]. Современные данные мировой литературы свидетельствуют о том, что главными участниками лимфоидной регуляции миогенеза являются Т-хелперы, а именно регуляторные Т-клетки (Treg), которые в большом количестве обнаруживаются вокруг регенерирующих мышечных волокон [12,13].…”

Section: Discussionunclassified

Xenogeneic lymphocytic RNA stimulates skeletal muscle regeneration

Tishevskaya

Головнева

Gallyamutdinov³

et al. 2021

RJTAO

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Objective: to find evidence of the existence of distant lymphocytic RNA control of physiological myogenesis as a way to control the muscle tissue regeneration process.Materials and methods. The study was conducted on male Wistar rats, n=33. In the first part of the experiment, 12 rats were subjected to regular 40-day physical activity (swimming), half of them were intraperitoneally injected 4 times with total RNA isolated from pig spleen lymphocytes at 30 days of age; 6 rats made up the intact control group. In histological preparations of different skeletal muscle groups, the width and cross-sectional area of muscle fibers, the area of nuclei, and the number of myocytes and myosatellite cells were evaluated. In the second part of the experiment, 15 intact rats were injected with the studied xenogeneic RNA and the amounts of ribonucleic acids in peripheral blood lymphocytes, spleen lymphocytes, and skeletal muscles were determined 2 hours and 24 hours after injection.Results. After the 40- day physical activity, the width of the fibers and the area of myocyte nuclei in the skeletal muscles increased; the absolute number of myosatellite cells and the area of their nuclei did not change. After administration of xenogenic RNA in the trained rats, in addition to an increase in the thickness and cross-sectional area of muscle fibers, the absolute number of myosatellite cells in m. biceps femoris, in m. triceps brachii, and in m. pectoralis major increased 1.4-fold, 1.3-fold, and 1.4-fold, respectively; the area of myosatellite nuclei increased on average by 7%. In intact rats, two hours after xenogeneic RNA injection, the amount of RNA in skeletal muscles remained unchanged, it increased by 19% in spleen lymphocytes, and by 16% in peripheral blood lymphocytes. At 24 hours, the RNA amount in the lymphocytes remained significantly higher than the control values, while in the muscle tissue, it didn’t differ from the control.Conclusion. Xenogeneic lymphocytic RNA stimulates physiological myogenesis by activating myosatellite cell proliferation.

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Section: Discussionunclassified

Xenogeneic lymphocytic RNA stimulates skeletal muscle regeneration

Tishevskaya

Головнева

Gallyamutdinov³

et al. 2021

RJTAO

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“…The integrity of the RNA was routinely evaluated using SDS-polyacrylamide gel electrophoresis according to Bertolini and De Lucca. 2 The gels were fixed in acetic acid and scanned for absorbance at 260nm in a spectrophotometer equipped with a linear transport.…”

Section: Methodsmentioning

confidence: 99%

“…There is considerable evidence indicating that exogenously supplied RNA molecules can be incorporated into eukaryotic cells and can exert a variety of biological effects both in vitro and in vivo. [1][2][3][4][5] Cell differentiation, genetic transformation and immunological effects induced by exogenous RNA have been investigated over the past few years. For example, RNA isolated from the endoderm of normal embryos of the amphibian A mbystoma mexicanum causes the differentiation of mutant heart tissue into functional, contractile cardiac muscle.…”

Section: Introductionmentioning

confidence: 99%

RNA from LPS‐stirnulated macrophages induces the release of tumour necrosis factor‐α and interleukin‐1 by resident macrophages

Ribeiro

Lucca

Flores

et al. 1993

Mediators of Inflammation

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The effect of exogenous RNA on many cellular functions has been studied in a variety of eukaryotic cells but there are few reports on macrophages. In the present study, it is demonstrated that cytoplasmatic RNA extracted from rat macrophages stimulated with Escherichia coli lipopolysaccharide (LPS), referred to as L-RNA, induced the release of TNF-α and IL-1 from monolayers of peritoneal resident macrophages. The activity of L-RNA was not altered by polymyxin B but was abolished by ribonuclease (RNase) pretreatment, indicating the absence of LPS contamination and that the integrity of the polynucleotide chain is essential for this activity. Both the poly A(−) and poly A(+) fractions obtained from L-RNA applied to oligo(dT)–cellulose chromatography induced TNF-α and IL-1 release. The L-RNA-induced cytokine release was inhibited by dexamethasone and seemed to be dependent on protein synthesis since this effect was abolished by cycloheximide or actinomycin-D. The LPS-stimulated macrophages, when pre-incubated with [5-3H]-uridine, secreted a trichloroacetic acid (TCA) precipitable material which was sensitive to RNase and KOH hydrolysis, suggesting that the material is RNA. This substance was also released from macrophage monolayers stimulated with IL-1β but not with TNF-α, IL-6 or IL-8. The substance secreted (3H-RNA) sediments in the 4–5S region of a 5–20% sucrose gradient. These results show that L-RNA induces cytokine secretion by macrophage monolayers and support the idea that, during inflammation, stimulated macrophages could release RNA which may further induce the release of cytokines by the resident cell population.

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“…A large volume of experimental data has been accumulated to show that not only viral (1) but also nonviral nucleic acids and synthetic polynucleotides can be taken up by mammalian cells in vitro (2)(3)(4)(5)(6)(7). Some of the evidence has met with skepticism (6,7) because of inadequate characterization of both the polynucleotide and the recipient cells.…”

Section: Introductionmentioning

confidence: 99%

Effect of polyamines on the uptake of poly (2′‐fluoro‐2′‐deoxyuridylic acid) by a mammalian cell line

Janik

Sommer

1976

J Supramol Struct

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VERO cells can take up poly(dUfl)1 from the medium. The uptake involves surface adsorption and, most probably, intracellular penetration. Part of the poly(dUfl) is hydrolyzed during incubation with the cells but the hydrolysis products are not incorporated into de novo synthesized nucleic acids. The uptake is reduced by serum and stimulated by polycationic ionenes. The magnitude of stimulation depends on the structure of the ionene and the treatment regimen.

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Physical and chemical characterization of RNA incorporated by rabbit spleen cells

Cited by 17 publications

References 18 publications

Xenogeneic lymphocytic RNA stimulates skeletal muscle regeneration

Xenogeneic lymphocytic RNA stimulates skeletal muscle regeneration

RNA from LPS‐stirnulated macrophages induces the release of tumour necrosis factor‐α and interleukin‐1 by resident macrophages

Effect of polyamines on the uptake of poly (2′‐fluoro‐2′‐deoxyuridylic acid) by a mammalian cell line

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