Physical and Chemical Adsorption of Mucor javanicus Lipase on SBA-15 Mesoporous Silica. Synthesis, Structural Characterization, and Activity Performance

Abstract

In this work a sample of SBA-15 mesoporous silica was synthesized and characterized by TEM, XRD, and N2 adsorption. The sample had a high value of specific surface area (1007 m2 g(-1)) and total pore volume (2.1 cm3 g(-1)). The pore diameter was 67 angstroms, so it was large enough to accommodate protein molecules inside the channels. Immobilization by physical adsorption of a commercial lipase preparation from Mucor javanicus was performed at different pH values (pH 5-8). pH 6 gave the highest lipase loading and hydrolytic activity of the corresponding biocatalyst. Chemical modification of the SBA-15 via glutardialdehyde allowed also the enzyme immobilization through chemical adsorption. This preparation was active toward tributyrin hydrolysis. On the contrary, very low activity toward triolein hydrolysis was observed. The reduction of the size of the channels due the immobilization process has been suggested as a possible explanation.

Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles

“…[36] Inhibiting lipase activity by increasing the pH values is probably due to an unfavorable charge distribution on the amino acid residues, that produces a further activity decrease. [24] Since the highest hydrolytic activity was observed when lipase adsorption was conducted at pH 6.8, all other steps of this work were carried out under these conditions.…”

“…[17,22,23] These parameters have large influence on the total amount of enzyme loading and enzyme-catalyst activity. [24,25] Hydrophobic binding of lipases by adsorption has proved successful due to its affinity for water/oil interfaces. Thus, the present work deals with the synthesis of PS nanoparticles and their use as a support for the immobilization of Cal-B.…”

Proteins in Mesoporous Silicates

“…9.0 MCM-41, Al-MCM-41 4-5 cytochrome P450 CYP2B4 [81] ca. 5.2 MCM-41, Al-MCM-41 4-5 penicillin acylase [98,99,104,107] 7.0 7.0 5.0 5.5 MCM-41, Al-MCM-41, SBA-15, KIT-6 3.3, 6-9 glucose oxidase [85,92,100] [102,116] ca. 6.2 -SBA-15, (bimodal HMMS) 6.7, (13, 37) Pseudomonas cepacia lipase [122] ca.…”

Investigating the mesostructure of ordered porous silica nanocomposites by transmission electron microscopy techniques

“…MCM-41 and SBA-15), possessing a hexagonal array of ordered mesopores has been widely investigated, partly due to the availability of large scale well-established synthetic protocols. [3,4] The corresponding cubic mesoporous silica having a cubic array of mesopores has the potential to be more efficient in catalysis and sorption processes, as the cubic array of pores is formed by a 3D cage-like system of interconnected pores providing easy accessibility in all directions. [5] However, cubic mesoporous silica has not been studied widely, as very controlled synthetic protocols are still lacking.…”